Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems
A native strain of the heterocytous cyanobacterium Trichormus variabilis VRUC 168 was mass cultivated in a low-cost photobioreactor for a combined production of Polyunsaturated Fatty Acids (PUFA) and Exopolymeric Substances (EPS) from the same cyanobacterial biomass. A sequential extraction protocol...
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| Format: | Article |
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MDPI AG
2018-08-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/16/9/298 |
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| author | Erika Bellini Matteo Ciocci Saverio Savio Simonetta Antonaroli Dror Seliktar Sonia Melino Roberta Congestri |
| author_facet | Erika Bellini Matteo Ciocci Saverio Savio Simonetta Antonaroli Dror Seliktar Sonia Melino Roberta Congestri |
| author_sort | Erika Bellini |
| collection | DOAJ |
| description | A native strain of the heterocytous cyanobacterium Trichormus variabilis VRUC 168 was mass cultivated in a low-cost photobioreactor for a combined production of Polyunsaturated Fatty Acids (PUFA) and Exopolymeric Substances (EPS) from the same cyanobacterial biomass. A sequential extraction protocol was optimized leading to high yields of Released EPS (REPS) and PUFA, useful for nutraceutical products and biomaterials. REPS were extracted and characterized by chemical staining, Reversed Phase-High-Performance Liquid Chromatography (RP-HPLC), Fourier Transform Infrared Spectroscopy (FT-IR) and other spectroscopic techniques. Due to their gelation property, REPS were used to produce a photo-polymerizable hybrid hydrogel (REPS-Hy) with addition of polyethylene glycol diacrylated (PEGDa). REPS-Hy was stable over time and resistant to dehydration and spontaneous hydrolysis. The rheological and functional properties of REPS-Hy were studied. The enzyme carrier ability of REPS-Hy was assessed using the detoxification enzyme thiosulfate:cyanide sulfur transferase (TST), suggesting the possibility to use REPS-Hy as an enzymatic hydrogel system. Finally, REPS-Hy was used as a scaffold for culturing human mesenchymal stem cells (hMSCs). The cell seeding onto the REPS-Hy and the cell embedding into 3D-REPS-Hy demonstrated a scaffolding property of REPS-Hy with non-cytotoxic effect, suggesting potential applications of cyanobacteria REPS for producing enzyme- and cell-carrier systems. |
| first_indexed | 2024-04-12T19:33:59Z |
| format | Article |
| id | doaj.art-42f7dcc3113f443aa277c6deb7867eb7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-12T19:33:59Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-42f7dcc3113f443aa277c6deb7867eb72022-12-22T03:19:15ZengMDPI AGMarine Drugs1660-33972018-08-0116929810.3390/md16090298md16090298Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier SystemsErika Bellini0Matteo Ciocci1Saverio Savio2Simonetta Antonaroli3Dror Seliktar4Sonia Melino5Roberta Congestri6Laboratory of Biology of Algae, Department of Biology, University of Rome Tor Vergata, 00133 Rome, ItalyDepartment of Chemical Science and Technologies, University of Rome Tor Vergata, 00133 Rome, ItalyLaboratory of Biology of Algae, Department of Biology, University of Rome Tor Vergata, 00133 Rome, ItalyDepartment of Chemical Science and Technologies, University of Rome Tor Vergata, 00133 Rome, ItalyDepartment of Biomedical Engineering, Technion Israel Institute of Technology, 3200003 Haifa, IsraelDepartment of Chemical Science and Technologies, University of Rome Tor Vergata, 00133 Rome, ItalyLaboratory of Biology of Algae, Department of Biology, University of Rome Tor Vergata, 00133 Rome, ItalyA native strain of the heterocytous cyanobacterium Trichormus variabilis VRUC 168 was mass cultivated in a low-cost photobioreactor for a combined production of Polyunsaturated Fatty Acids (PUFA) and Exopolymeric Substances (EPS) from the same cyanobacterial biomass. A sequential extraction protocol was optimized leading to high yields of Released EPS (REPS) and PUFA, useful for nutraceutical products and biomaterials. REPS were extracted and characterized by chemical staining, Reversed Phase-High-Performance Liquid Chromatography (RP-HPLC), Fourier Transform Infrared Spectroscopy (FT-IR) and other spectroscopic techniques. Due to their gelation property, REPS were used to produce a photo-polymerizable hybrid hydrogel (REPS-Hy) with addition of polyethylene glycol diacrylated (PEGDa). REPS-Hy was stable over time and resistant to dehydration and spontaneous hydrolysis. The rheological and functional properties of REPS-Hy were studied. The enzyme carrier ability of REPS-Hy was assessed using the detoxification enzyme thiosulfate:cyanide sulfur transferase (TST), suggesting the possibility to use REPS-Hy as an enzymatic hydrogel system. Finally, REPS-Hy was used as a scaffold for culturing human mesenchymal stem cells (hMSCs). The cell seeding onto the REPS-Hy and the cell embedding into 3D-REPS-Hy demonstrated a scaffolding property of REPS-Hy with non-cytotoxic effect, suggesting potential applications of cyanobacteria REPS for producing enzyme- and cell-carrier systems.http://www.mdpi.com/1660-3397/16/9/298Extracellular Polymeric Substanceshydrogelmesenchymal stem cellsbiomaterialsenzymeomega 3PUFATrichormus variabilisCyanobacteria |
| spellingShingle | Erika Bellini Matteo Ciocci Saverio Savio Simonetta Antonaroli Dror Seliktar Sonia Melino Roberta Congestri Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems Marine Drugs Extracellular Polymeric Substances hydrogel mesenchymal stem cells biomaterials enzyme omega 3 PUFA Trichormus variabilis Cyanobacteria |
| title | Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems |
| title_full | Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems |
| title_fullStr | Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems |
| title_full_unstemmed | Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems |
| title_short | Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems |
| title_sort | trichormus variabilis cyanobacteria biomass from the nutraceutical products to novel eps cell protein carrier systems |
| topic | Extracellular Polymeric Substances hydrogel mesenchymal stem cells biomaterials enzyme omega 3 PUFA Trichormus variabilis Cyanobacteria |
| url | http://www.mdpi.com/1660-3397/16/9/298 |
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