In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria

Probiotics are live microorganisms that confer a health benefit to the host when administered in adequate amounts, with the main site of action at the intestinal level. In order to have a maximal effect, probiotic bacteria have to prove a high tolerance for the conditions present in the upper gastro...

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Main Authors: Maria Cătălina MATEI LAȚIU, Victoria BUZA, Flore CHIRILĂ, Zsolt BOROS, Călin LAȚIU, Andrei Radu SZAKACS, Laura Cristina ȘTEFĂNUȚ
Format: Article
Language:English
Published: AcademicPres 2022-05-01
Series:Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine
Subjects:
Online Access:https://journals.usamvcluj.ro/index.php/veterinary/article/view/14385
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author Maria Cătălina MATEI LAȚIU
Victoria BUZA
Flore CHIRILĂ
Zsolt BOROS
Călin LAȚIU
Andrei Radu SZAKACS
Laura Cristina ȘTEFĂNUȚ
author_facet Maria Cătălina MATEI LAȚIU
Victoria BUZA
Flore CHIRILĂ
Zsolt BOROS
Călin LAȚIU
Andrei Radu SZAKACS
Laura Cristina ȘTEFĂNUȚ
author_sort Maria Cătălina MATEI LAȚIU
collection DOAJ
description Probiotics are live microorganisms that confer a health benefit to the host when administered in adequate amounts, with the main site of action at the intestinal level. In order to have a maximal effect, probiotic bacteria have to prove a high tolerance for the conditions present in the upper gastro-intestinal tract. The aim of the present study was to assess, from a qualitative point of view, the tolerance of different probiotic bacterial strains and combinations to simulated gastric juice, bile, fructose, lactose and glucose. Also, the influence of food on the bacterial growth after artificial digestion was analyzed. A bacteriologic assessment was realized for three different commercial probiotic products (Bacillus subtilis + Bacillus licheniformis + Pediococcus acidilactici; Enterococcus faecium; Bacillus subtilis + B. licheniformis + B.coagulans + B.indicus + B. clausii), simulating different conditions (artificial digestion method in the absence/presence of food, presence of bile, fructose, glucose, lactose). The obtained results suggested that the tolerance to simulated gastric juice is higher if the food is present in the moment of digestion, regardless of the probiotic bacterial strain or combination. Also, the tolerance to bile was different from one combination to another. The influence of fructose, lactose and glucose are not remarkable from a qualitative point of view. Moreover, the growth of different probiotic bacterial strains is influenced by the simulated gastro-intestinal conditions, sporulated bacteria presenting a slightly higher tolerance compared with non-sporulated strains.
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spelling doaj.art-4301a92ce9a44b3cbd32bb650aa3e91a2022-12-22T00:35:49ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine1843-52701843-53782022-05-01791717610.15835/buasvmcn-vm:2021.002211714In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic BacteriaMaria Cătălina MATEI LAȚIU0Victoria BUZA1Flore CHIRILĂ2Zsolt BOROS3Călin LAȚIU4Andrei Radu SZAKACS5Laura Cristina ȘTEFĂNUȚ6University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaProbiotics are live microorganisms that confer a health benefit to the host when administered in adequate amounts, with the main site of action at the intestinal level. In order to have a maximal effect, probiotic bacteria have to prove a high tolerance for the conditions present in the upper gastro-intestinal tract. The aim of the present study was to assess, from a qualitative point of view, the tolerance of different probiotic bacterial strains and combinations to simulated gastric juice, bile, fructose, lactose and glucose. Also, the influence of food on the bacterial growth after artificial digestion was analyzed. A bacteriologic assessment was realized for three different commercial probiotic products (Bacillus subtilis + Bacillus licheniformis + Pediococcus acidilactici; Enterococcus faecium; Bacillus subtilis + B. licheniformis + B.coagulans + B.indicus + B. clausii), simulating different conditions (artificial digestion method in the absence/presence of food, presence of bile, fructose, glucose, lactose). The obtained results suggested that the tolerance to simulated gastric juice is higher if the food is present in the moment of digestion, regardless of the probiotic bacterial strain or combination. Also, the tolerance to bile was different from one combination to another. The influence of fructose, lactose and glucose are not remarkable from a qualitative point of view. Moreover, the growth of different probiotic bacterial strains is influenced by the simulated gastro-intestinal conditions, sporulated bacteria presenting a slightly higher tolerance compared with non-sporulated strains.https://journals.usamvcluj.ro/index.php/veterinary/article/view/14385bacterial tolerancegastro-intestinal tractprobioticssimulated conditions.
spellingShingle Maria Cătălina MATEI LAȚIU
Victoria BUZA
Flore CHIRILĂ
Zsolt BOROS
Călin LAȚIU
Andrei Radu SZAKACS
Laura Cristina ȘTEFĂNUȚ
In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine
bacterial tolerance
gastro-intestinal tract
probiotics
simulated conditions.
title In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria
title_full In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria
title_fullStr In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria
title_full_unstemmed In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria
title_short In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria
title_sort in vitro qualitative assessment of tolerance to simulated gastric juice bile fructose glucose and lactose for different probiotic bacteria
topic bacterial tolerance
gastro-intestinal tract
probiotics
simulated conditions.
url https://journals.usamvcluj.ro/index.php/veterinary/article/view/14385
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