Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea
Gray mold caused by Botrytis cinerea is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain Bacillus K1 with excellently antagonistic B. cinerea from the wild grape endosphere...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.935675/full |
| _version_ | 1818189689250643968 |
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| author | Peiqian Li Baozhen Feng Zhen Yao Bohui Wei Yanfei Zhao Shouguo Shi |
| author_facet | Peiqian Li Baozhen Feng Zhen Yao Bohui Wei Yanfei Zhao Shouguo Shi |
| author_sort | Peiqian Li |
| collection | DOAJ |
| description | Gray mold caused by Botrytis cinerea is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain Bacillus K1 with excellently antagonistic B. cinerea from the wild grape endosphere. We identified a wild grape endophytic strain K1 with high antifungal activity against B. cinerea both in vitro and in vivo. Combining the phylogenetic results based on 16S rDNA and genome sequencing, K1 was assigned as Bacillus subtilis. The in vitro results displayed that K1 and its volatile substances could significantly inhibit the mycelia growth of B. cinerea. Grape fruit inoculated with Bacillus K1 showed lower gray mold during treatment. The higher levels of defense-related enzymes, including peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase, were induced in grapes after inoculation. Scanning electron microscopy (SEM) suggested that K1 inhibited mycelial growth via bacterial colonization and antibiosis in grapes. The gas chromatography–mass spectrometry analysis identified 33 volatiles in which dibutyl phthalate was the major compound accounting for 74.28%. Dibutyl phthalate demonstrated strong activity in suppressing the mycelia growth of B. cinerea. Genome bioinformatics analysis revealed that the K1 chromosome harbored many known biosynthesis gene clusters encoding subtilosin, bacillaene, bacillibactin, bacilysin, and fengycin. This study provides a potential biological agent to control diseases of post-harvest grape fruit and improves our understanding of the possible biocontrol mechanisms of the Bacillus strain. |
| first_indexed | 2024-12-11T23:46:48Z |
| format | Article |
| id | doaj.art-4304875b2595410eb652101b1cebb979 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T23:46:48Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4304875b2595410eb652101b1cebb9792022-12-22T00:45:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.935675935675Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinereaPeiqian LiBaozhen FengZhen YaoBohui WeiYanfei ZhaoShouguo ShiGray mold caused by Botrytis cinerea is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain Bacillus K1 with excellently antagonistic B. cinerea from the wild grape endosphere. We identified a wild grape endophytic strain K1 with high antifungal activity against B. cinerea both in vitro and in vivo. Combining the phylogenetic results based on 16S rDNA and genome sequencing, K1 was assigned as Bacillus subtilis. The in vitro results displayed that K1 and its volatile substances could significantly inhibit the mycelia growth of B. cinerea. Grape fruit inoculated with Bacillus K1 showed lower gray mold during treatment. The higher levels of defense-related enzymes, including peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase, were induced in grapes after inoculation. Scanning electron microscopy (SEM) suggested that K1 inhibited mycelial growth via bacterial colonization and antibiosis in grapes. The gas chromatography–mass spectrometry analysis identified 33 volatiles in which dibutyl phthalate was the major compound accounting for 74.28%. Dibutyl phthalate demonstrated strong activity in suppressing the mycelia growth of B. cinerea. Genome bioinformatics analysis revealed that the K1 chromosome harbored many known biosynthesis gene clusters encoding subtilosin, bacillaene, bacillibactin, bacilysin, and fengycin. This study provides a potential biological agent to control diseases of post-harvest grape fruit and improves our understanding of the possible biocontrol mechanisms of the Bacillus strain.https://www.frontiersin.org/articles/10.3389/fmicb.2022.935675/fullgrapeendophyteBacillusantifungal activityVOCsbiosynthetic gene clusters |
| spellingShingle | Peiqian Li Baozhen Feng Zhen Yao Bohui Wei Yanfei Zhao Shouguo Shi Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea Frontiers in Microbiology grape endophyte Bacillus antifungal activity VOCs biosynthetic gene clusters |
| title | Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea |
| title_full | Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea |
| title_fullStr | Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea |
| title_full_unstemmed | Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea |
| title_short | Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea |
| title_sort | antifungal activity of endophytic bacillus k1 against botrytis cinerea |
| topic | grape endophyte Bacillus antifungal activity VOCs biosynthetic gene clusters |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.935675/full |
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