Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds
In recent years, aqueous two-phase systems (ATPSs) have been widely used in different fields and have become an increasingly attractive subject due to their application in the separation and purification of biomolecules. In this work, the aqueous phase behavior of ionic liquids (ILs) was modulated b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/16/5307 |
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| author | Wenzhuo Xu Xinpei Gao Liqiang Zheng Fei Lu |
| author_facet | Wenzhuo Xu Xinpei Gao Liqiang Zheng Fei Lu |
| author_sort | Wenzhuo Xu |
| collection | DOAJ |
| description | In recent years, aqueous two-phase systems (ATPSs) have been widely used in different fields and have become an increasingly attractive subject due to their application in the separation and purification of biomolecules. In this work, the aqueous phase behavior of ionic liquids (ILs) was modulated by changing the <i>cis</i>-<i>trans</i> structure of the anion in ILs. With the same tetra-butyl-phosphine as the cation, the cis-anion exhibited upper critical solution temperature (UCST) phenomena. In contrast, the <i>trans</i>-anion exhibited lower critical solution temperature (LCST) phenomena. The proposed mechanism shows that the main factors responsible for these phenomena include variations in the dissociation degree with temperature and the steric hindrance of the ILs. This phase behavior combines the chemical equilibrium in a solution with the microstructure of the molecule and is useful for constructing new chemical dynamic equilibria in ATPS. As an example of its application, aqueous solutions of both ILs can be used for the efficient separation and extraction of specific amino acids. The two ATPS systems reported in this work highlight a simple, effective, and environmentally friendly method for separating small biological molecules. |
| first_indexed | 2024-03-09T09:51:47Z |
| format | Article |
| id | doaj.art-4305859b90d74928a7c7919aaa35b792 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T09:51:47Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4305859b90d74928a7c7919aaa35b7922023-12-02T00:05:16ZengMDPI AGMolecules1420-30492022-08-012716530710.3390/molecules27165307Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen BondsWenzhuo Xu0Xinpei Gao1Liqiang Zheng2Fei Lu3Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan 250100, ChinaKey Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources, Hainan University, No 58 Renmin Avenue, Haikou 570228, ChinaKey Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan 250100, ChinaKey Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources, Hainan University, No 58 Renmin Avenue, Haikou 570228, ChinaIn recent years, aqueous two-phase systems (ATPSs) have been widely used in different fields and have become an increasingly attractive subject due to their application in the separation and purification of biomolecules. In this work, the aqueous phase behavior of ionic liquids (ILs) was modulated by changing the <i>cis</i>-<i>trans</i> structure of the anion in ILs. With the same tetra-butyl-phosphine as the cation, the cis-anion exhibited upper critical solution temperature (UCST) phenomena. In contrast, the <i>trans</i>-anion exhibited lower critical solution temperature (LCST) phenomena. The proposed mechanism shows that the main factors responsible for these phenomena include variations in the dissociation degree with temperature and the steric hindrance of the ILs. This phase behavior combines the chemical equilibrium in a solution with the microstructure of the molecule and is useful for constructing new chemical dynamic equilibria in ATPS. As an example of its application, aqueous solutions of both ILs can be used for the efficient separation and extraction of specific amino acids. The two ATPS systems reported in this work highlight a simple, effective, and environmentally friendly method for separating small biological molecules.https://www.mdpi.com/1420-3049/27/16/5307ionic liquidaqueous two-phase systemdicarboxylic acidamino acid isolation |
| spellingShingle | Wenzhuo Xu Xinpei Gao Liqiang Zheng Fei Lu Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds Molecules ionic liquid aqueous two-phase system dicarboxylic acid amino acid isolation |
| title | Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds |
| title_full | Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds |
| title_fullStr | Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds |
| title_full_unstemmed | Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds |
| title_short | Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds |
| title_sort | ionic liquid based aqueous two phase systems induced by intra and intermolecular hydrogen bonds |
| topic | ionic liquid aqueous two-phase system dicarboxylic acid amino acid isolation |
| url | https://www.mdpi.com/1420-3049/27/16/5307 |
| work_keys_str_mv | AT wenzhuoxu ionicliquidbasedaqueoustwophasesystemsinducedbyintraandintermolecularhydrogenbonds AT xinpeigao ionicliquidbasedaqueoustwophasesystemsinducedbyintraandintermolecularhydrogenbonds AT liqiangzheng ionicliquidbasedaqueoustwophasesystemsinducedbyintraandintermolecularhydrogenbonds AT feilu ionicliquidbasedaqueoustwophasesystemsinducedbyintraandintermolecularhydrogenbonds |