Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male Rats
Background: Myocardial infarction (MI) is one of the most important causes of death worldwide. The effect of flunixin meglumine (FM) on MI is unknown. We investigated the protective effect of FM on the serum biochemical profile, malondialdehyde (MDA), and heart histology changes induced by isoprot...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2019-10-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/article-1-762-en.pdf |
| _version_ | 1818058704987095040 |
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| author | Shima Tahery Mahmood Ahmadi-Hamedani* Keivan Keramati Abbas Javaheri Vayghan Saeideh Naeimi |
| author_facet | Shima Tahery Mahmood Ahmadi-Hamedani* Keivan Keramati Abbas Javaheri Vayghan Saeideh Naeimi |
| author_sort | Shima Tahery |
| collection | DOAJ |
| description | Background:
Myocardial infarction (MI) is one of the most important causes of death worldwide. The effect of flunixin meglumine (FM) on MI is unknown. We investigated the protective effect of FM on the serum biochemical profile, malondialdehyde (MDA), and heart histology changes induced by isoproterenol (ISO) in adult male rats.
Methods:
Rats were randomly divided into four groups of six each as follows: Group 1, control. Group 2, was administered ISO subcutaneously at 85 mg/kg for 2 days. Group 3 was administered FM intraperitoneally at 2 mg/kg/d, for 3 days; and Group 4 was administered FM 2mg/kg/d intraperitoneally for 3 days and ISO 85mg/kg subcutaneously for two consecutive days. Blood samples were collected after anesthesia, 24h after the second ISO administration. The heart tissue was immediately removed for histopathological examination. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), Blood urea nitrogen (BUN), creatinine and serum MDA levels were measured on an autoanalyzer and spectrophotometer.
Results:
In Group 4 (FM+ISO) the levels of AST, LDH and MDA decreased compared with those for Group 2 (ISO) (P< 0.05). Histopathologic tests revealed that ISO induced myocardial injury in Group 3 rats, whereas the alterations of the heart architecture were improved by FM in Group 4 (FM+ISO).
Conclusions:
The results suggest that FM has a potential cardioprotective effect against an experimental model of MI in rats. |
| first_indexed | 2024-12-10T13:04:52Z |
| format | Article |
| id | doaj.art-4305f19fa6f34751b38fd82f34724693 |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-12-10T13:04:52Z |
| publishDate | 2019-10-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-4305f19fa6f34751b38fd82f347246932022-12-22T01:47:52ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592019-10-011343337Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male RatsShima Tahery 0Mahmood Ahmadi-Hamedani*1Keivan Keramati 2Abbas Javaheri Vayghan3Saeideh Naeimi 4DVM Student, Veterinary Medicine, Semnan University. Semnan, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University. Semnan, Iran. E-mail:ahmadi.hamedani@semnan.ac.irDepartment of Basic Sciences, Faculty of Veterinary Medicine, Semnan University. Semnan, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University. Semnan, Iran.Department of Basic Sciences, Faculty of Veterinary Medicine, Semnan University. Semnan, Iran.Background: Myocardial infarction (MI) is one of the most important causes of death worldwide. The effect of flunixin meglumine (FM) on MI is unknown. We investigated the protective effect of FM on the serum biochemical profile, malondialdehyde (MDA), and heart histology changes induced by isoproterenol (ISO) in adult male rats. Methods: Rats were randomly divided into four groups of six each as follows: Group 1, control. Group 2, was administered ISO subcutaneously at 85 mg/kg for 2 days. Group 3 was administered FM intraperitoneally at 2 mg/kg/d, for 3 days; and Group 4 was administered FM 2mg/kg/d intraperitoneally for 3 days and ISO 85mg/kg subcutaneously for two consecutive days. Blood samples were collected after anesthesia, 24h after the second ISO administration. The heart tissue was immediately removed for histopathological examination. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), Blood urea nitrogen (BUN), creatinine and serum MDA levels were measured on an autoanalyzer and spectrophotometer. Results: In Group 4 (FM+ISO) the levels of AST, LDH and MDA decreased compared with those for Group 2 (ISO) (P< 0.05). Histopathologic tests revealed that ISO induced myocardial injury in Group 3 rats, whereas the alterations of the heart architecture were improved by FM in Group 4 (FM+ISO). Conclusions: The results suggest that FM has a potential cardioprotective effect against an experimental model of MI in rats.http://ijt.arakmu.ac.ir/article-1-762-en.pdfcardioprotective effectfunixin meglumineisoproterenolmdamyocardial infarction |
| spellingShingle | Shima Tahery Mahmood Ahmadi-Hamedani* Keivan Keramati Abbas Javaheri Vayghan Saeideh Naeimi Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male Rats Iranian Journal of Toxicology cardioprotective effect funixin meglumine isoproterenol mda myocardial infarction |
| title | Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male Rats |
| title_full | Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male Rats |
| title_fullStr | Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male Rats |
| title_full_unstemmed | Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male Rats |
| title_short | Protective Effect of Flunixin meglumin on Changes Induced by Isoproterenol in Serum Biochemical Profile, Malondialdehyde and Heart Histology of Adult Male Rats |
| title_sort | protective effect of flunixin meglumin on changes induced by isoproterenol in serum biochemical profile malondialdehyde and heart histology of adult male rats |
| topic | cardioprotective effect funixin meglumine isoproterenol mda myocardial infarction |
| url | http://ijt.arakmu.ac.ir/article-1-762-en.pdf |
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