The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows
Abstract Background The aim of this study was to investigate the association between plasma β-hydroxybutyric acid (BHBA) and conjugated linoleic acid in postpartum Polish Holstein-Friesian (PHF) cows. The experiment was carried out at an experimental dairy farm, where a herd of approximately 350 cow...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-019-2131-2 |
| _version_ | 1819054416093773824 |
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| author | Kamila Puppel Marcin Gołębiewski Paweł Solarczyk Grzegorz Grodkowski Jan Slósarz Małgorzata Kunowska-Slósarz Marek Balcerak Tomasz Przysucha Aleksandra Kalińska Beata Kuczyńska |
| author_facet | Kamila Puppel Marcin Gołębiewski Paweł Solarczyk Grzegorz Grodkowski Jan Slósarz Małgorzata Kunowska-Slósarz Marek Balcerak Tomasz Przysucha Aleksandra Kalińska Beata Kuczyńska |
| author_sort | Kamila Puppel |
| collection | DOAJ |
| description | Abstract Background The aim of this study was to investigate the association between plasma β-hydroxybutyric acid (BHBA) and conjugated linoleic acid in postpartum Polish Holstein-Friesian (PHF) cows. The experiment was carried out at an experimental dairy farm, where a herd of approximately 350 cows was kept. Samples were taken at six time points: between days 5–7, 8–14, 15–21, 22–28, 29–35, and 36–42, resulting in 510 samples of both milk and blood. The cows involved in the experiment were divided into two groups – ketotic and healthy – by taking into account general health symptoms, blood serum BHBA, and non-esterified fatty acids (NEFA) concentration at 5–7 days postpartum. Results In the first week of lactation, at 5–7 day in milk (DIM), the study showed a 53% lower level of C18:2 cis-9 trans-11 (CLA9) and an 80% lower level of C18:2 trans-10 cis-12 (CLA10) in cows with diagnosed ketosis compared to healthy cows. In the second week of lactation (8–14 DIM), a 34% lower level of CLA9 and a 54% lower level of CLA10 was found in the group of cows with BHBA levels > 1.2 mmol/L. Additionally, Pearson correlation analysis showed significant negative correlation between BHBA x CLA9 and BHBA x CLA10 in the first week of lactation: − 0.732and − 0.821, respectively. Conclusion The study shows that that both CLA9 and CLA10 can be used as markers for the early diagnosis of elevated blood levels of BHBA in postpartum Polish Holstein-Friesian cows. |
| first_indexed | 2024-12-21T12:51:16Z |
| format | Article |
| id | doaj.art-430728314e9d458cb0db86f28e4190f3 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T12:51:16Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-430728314e9d458cb0db86f28e4190f32022-12-21T19:03:29ZengBMCBMC Veterinary Research1746-61482019-10-0115111110.1186/s12917-019-2131-2The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cowsKamila Puppel0Marcin Gołębiewski1Paweł Solarczyk2Grzegorz Grodkowski3Jan Slósarz4Małgorzata Kunowska-Slósarz5Marek Balcerak6Tomasz Przysucha7Aleksandra Kalińska8Beata Kuczyńska9Animal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAnimal Breeding and Production Department, Warsaw University of Life SciencesAbstract Background The aim of this study was to investigate the association between plasma β-hydroxybutyric acid (BHBA) and conjugated linoleic acid in postpartum Polish Holstein-Friesian (PHF) cows. The experiment was carried out at an experimental dairy farm, where a herd of approximately 350 cows was kept. Samples were taken at six time points: between days 5–7, 8–14, 15–21, 22–28, 29–35, and 36–42, resulting in 510 samples of both milk and blood. The cows involved in the experiment were divided into two groups – ketotic and healthy – by taking into account general health symptoms, blood serum BHBA, and non-esterified fatty acids (NEFA) concentration at 5–7 days postpartum. Results In the first week of lactation, at 5–7 day in milk (DIM), the study showed a 53% lower level of C18:2 cis-9 trans-11 (CLA9) and an 80% lower level of C18:2 trans-10 cis-12 (CLA10) in cows with diagnosed ketosis compared to healthy cows. In the second week of lactation (8–14 DIM), a 34% lower level of CLA9 and a 54% lower level of CLA10 was found in the group of cows with BHBA levels > 1.2 mmol/L. Additionally, Pearson correlation analysis showed significant negative correlation between BHBA x CLA9 and BHBA x CLA10 in the first week of lactation: − 0.732and − 0.821, respectively. Conclusion The study shows that that both CLA9 and CLA10 can be used as markers for the early diagnosis of elevated blood levels of BHBA in postpartum Polish Holstein-Friesian cows.http://link.springer.com/article/10.1186/s12917-019-2131-2Cowβ-Hydroxybutyric acidConjugated linoleic acidKetosisMilk |
| spellingShingle | Kamila Puppel Marcin Gołębiewski Paweł Solarczyk Grzegorz Grodkowski Jan Slósarz Małgorzata Kunowska-Slósarz Marek Balcerak Tomasz Przysucha Aleksandra Kalińska Beata Kuczyńska The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows BMC Veterinary Research Cow β-Hydroxybutyric acid Conjugated linoleic acid Ketosis Milk |
| title | The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows |
| title_full | The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows |
| title_fullStr | The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows |
| title_full_unstemmed | The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows |
| title_short | The relationship between plasma β-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows |
| title_sort | relationship between plasma β hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum polish holstein friesian cows |
| topic | Cow β-Hydroxybutyric acid Conjugated linoleic acid Ketosis Milk |
| url | http://link.springer.com/article/10.1186/s12917-019-2131-2 |
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