Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil
Contamination of soil by crude oil can pose serious problems to ecosystems. Soil washing by solutions containing biosurfactants is one of the most efficient methods for the remediation of contaminated soil by crude oil because it removes not only the crude oil but also heavy metals. In this study, f...
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| Format: | Article |
| Language: | English |
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Water and Wastewater Consulting Engineers Research Development
2010-09-01
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| Series: | آب و فاضلاب |
| Subjects: | |
| Online Access: | http://www.wwjournal.ir/article_1279_b8eef23316263ebe8f86cfa3906b570e.pdf |
| _version_ | 1818740952786796544 |
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| author | Nader Mokhtarian Amir Reza Talaie Nematolah Jaafarzadeh Mohammad Reza Talaie Masoud Beheshti |
| author_facet | Nader Mokhtarian Amir Reza Talaie Nematolah Jaafarzadeh Mohammad Reza Talaie Masoud Beheshti |
| author_sort | Nader Mokhtarian |
| collection | DOAJ |
| description | Contamination of soil by crude oil can pose serious problems to ecosystems. Soil washing by solutions containing biosurfactants is one of the most efficient methods for the remediation of contaminated soil by crude oil because it removes not only the crude oil but also heavy metals. In this study, five soil samples were taken from fields exposed to oil compounds over the years in order to produce biosurfactants from microorganisms that were capable of degrading oil compounds. Sixteen such microorganisms were isolated. After cultivation, their emulsification strength was examined using E24 test. From among the experimental microorganisms, a gram-negative and rod-shape microorganism called A-12 showed the greatest value of the E24 test index (36%). For each liter of the culture medium containing 365 mg of microorganisms, 3 gr of the biosurfactant compound was produced and separated as dried powder. The purified biosurfactant was used in the soil washing process. Also, the insulated microorganisms were capable of degrading crude oil floating on wastewaters. |
| first_indexed | 2024-12-18T01:48:54Z |
| format | Article |
| id | doaj.art-430903074917488d827f038ea4b71abb |
| institution | Directory Open Access Journal |
| issn | 1024-5936 2383-0905 |
| language | English |
| last_indexed | 2024-12-18T01:48:54Z |
| publishDate | 2010-09-01 |
| publisher | Water and Wastewater Consulting Engineers Research Development |
| record_format | Article |
| series | آب و فاضلاب |
| spelling | doaj.art-430903074917488d827f038ea4b71abb2022-12-21T21:25:06ZengWater and Wastewater Consulting Engineers Research Developmentآب و فاضلاب1024-59362383-09052010-09-0121320271279Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated SoilNader Mokhtarian0Amir Reza Talaie1Nematolah Jaafarzadeh2Mohammad Reza Talaie3Masoud Beheshti4M.Sc. and Academic Member of Chemistry Eng. Dept., Azad University, Branch of ShahrezaM.Sc. and Academic Member of Civil and Environmental Eng., Jami Institute of Tech., DelijanAssoc. Prof. of Environmental Health Eng., Faculty of Public Health, Ahwaz University of Medical Sciences, AhwazAssoc. Prof. of Chemical Eng., Dept. of Eng., Isfahan University, IsfahanAssist. Prof. of Chemical Eng., Dept. of Eng., Isfahan University, IsfahanContamination of soil by crude oil can pose serious problems to ecosystems. Soil washing by solutions containing biosurfactants is one of the most efficient methods for the remediation of contaminated soil by crude oil because it removes not only the crude oil but also heavy metals. In this study, five soil samples were taken from fields exposed to oil compounds over the years in order to produce biosurfactants from microorganisms that were capable of degrading oil compounds. Sixteen such microorganisms were isolated. After cultivation, their emulsification strength was examined using E24 test. From among the experimental microorganisms, a gram-negative and rod-shape microorganism called A-12 showed the greatest value of the E24 test index (36%). For each liter of the culture medium containing 365 mg of microorganisms, 3 gr of the biosurfactant compound was produced and separated as dried powder. The purified biosurfactant was used in the soil washing process. Also, the insulated microorganisms were capable of degrading crude oil floating on wastewaters.http://www.wwjournal.ir/article_1279_b8eef23316263ebe8f86cfa3906b570e.pdfCrude OilBiosurfactantBioemulsifierE24 |
| spellingShingle | Nader Mokhtarian Amir Reza Talaie Nematolah Jaafarzadeh Mohammad Reza Talaie Masoud Beheshti Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil آب و فاضلاب Crude Oil Biosurfactant Bioemulsifier E24 |
| title | Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil |
| title_full | Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil |
| title_fullStr | Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil |
| title_full_unstemmed | Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil |
| title_short | Producing Biosurfactants from Purified Microorganisms Obtained from Oil-contaminated Soil |
| title_sort | producing biosurfactants from purified microorganisms obtained from oil contaminated soil |
| topic | Crude Oil Biosurfactant Bioemulsifier E24 |
| url | http://www.wwjournal.ir/article_1279_b8eef23316263ebe8f86cfa3906b570e.pdf |
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