Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet
The objective of this study was to investigate the antioxidant and hepatoprotective effect of the chronic use of conventional (CGJ) or organic (OGJ) grape juice from the Bordeaux variety grape on oxidative stress and cytoarchitecture in the liver of rats supplemented with a high-fat diet (HFD) for t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-04-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/3/2/323 |
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| author | Iselde Buchner Niara Medeiros Denise dos Santos Lacerda Carlos Augusto B. M. Normann Tanise Gemelli Paula Rigon Clovis Milton Duval Wannmacher João Antônio Pegas Henriques Caroline Dani Cláudia Funchal |
| author_facet | Iselde Buchner Niara Medeiros Denise dos Santos Lacerda Carlos Augusto B. M. Normann Tanise Gemelli Paula Rigon Clovis Milton Duval Wannmacher João Antônio Pegas Henriques Caroline Dani Cláudia Funchal |
| author_sort | Iselde Buchner |
| collection | DOAJ |
| description | The objective of this study was to investigate the antioxidant and hepatoprotective effect of the chronic use of conventional (CGJ) or organic (OGJ) grape juice from the Bordeaux variety grape on oxidative stress and cytoarchitecture in the liver of rats supplemented with a high-fat diet (HFD) for three months. The results demonstrated that HFD induced an increase in thiobarbituric acid-reactive substances (TBARS), catalase (CAT) activity and 2′,7′-dihydrodichlorofluorescein (DCFH) oxidation and a decrease in sulfhydryl content and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. HFD also induced hepatocellular degeneration and steatosis. These alterations were prevented by CGJ and OGJ, where OGJ was more effective. Therefore, it was concluded that HFD induced oxidative stress and liver damage and that the chronic use of grape juice was able to prevent these alterations. |
| first_indexed | 2024-03-12T09:06:33Z |
| format | Article |
| id | doaj.art-430b5fde9e754f8ba6e96b21e15f3760 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T09:06:33Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-430b5fde9e754f8ba6e96b21e15f37602023-09-02T15:16:46ZengMDPI AGAntioxidants2076-39212014-04-013232333810.3390/antiox3020323antiox3020323Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat DietIselde Buchner0Niara Medeiros1Denise dos Santos Lacerda2Carlos Augusto B. M. Normann3Tanise Gemelli4Paula Rigon5Clovis Milton Duval Wannmacher6João Antônio Pegas Henriques7Caroline Dani8Cláudia Funchal9Centro Universitário Metodista do IPA, 90420-060 Porto Alegre, BrazilCentro Universitário Metodista do IPA, 90420-060 Porto Alegre, BrazilCentro Universitário Metodista do IPA, 90420-060 Porto Alegre, BrazilCentro Universitário Metodista do IPA, 90420-060 Porto Alegre, BrazilDepartamento de Bioquímica, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, BrazilDepartamento de Ciências Morfológicas, Universidade Federal do Rio Grande do Sul, 90040-060 Porto Alegre, BrazilDepartamento de Bioquímica, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, BrazilDepartamento de Biofísica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, BrazilCentro Universitário Metodista do IPA, 90420-060 Porto Alegre, BrazilCentro Universitário Metodista do IPA, 90420-060 Porto Alegre, BrazilThe objective of this study was to investigate the antioxidant and hepatoprotective effect of the chronic use of conventional (CGJ) or organic (OGJ) grape juice from the Bordeaux variety grape on oxidative stress and cytoarchitecture in the liver of rats supplemented with a high-fat diet (HFD) for three months. The results demonstrated that HFD induced an increase in thiobarbituric acid-reactive substances (TBARS), catalase (CAT) activity and 2′,7′-dihydrodichlorofluorescein (DCFH) oxidation and a decrease in sulfhydryl content and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. HFD also induced hepatocellular degeneration and steatosis. These alterations were prevented by CGJ and OGJ, where OGJ was more effective. Therefore, it was concluded that HFD induced oxidative stress and liver damage and that the chronic use of grape juice was able to prevent these alterations.http://www.mdpi.com/2076-3921/3/2/323liverantioxidantsgrapeshigh-fat dietfree radicals |
| spellingShingle | Iselde Buchner Niara Medeiros Denise dos Santos Lacerda Carlos Augusto B. M. Normann Tanise Gemelli Paula Rigon Clovis Milton Duval Wannmacher João Antônio Pegas Henriques Caroline Dani Cláudia Funchal Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet Antioxidants liver antioxidants grapes high-fat diet free radicals |
| title | Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet |
| title_full | Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet |
| title_fullStr | Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet |
| title_full_unstemmed | Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet |
| title_short | Hepatoprotective and Antioxidant Potential of Organic and Conventional Grape Juices in Rats Fed a High-Fat Diet |
| title_sort | hepatoprotective and antioxidant potential of organic and conventional grape juices in rats fed a high fat diet |
| topic | liver antioxidants grapes high-fat diet free radicals |
| url | http://www.mdpi.com/2076-3921/3/2/323 |
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