Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells
Objective To explore the effect of KCNQ1OT1 gene knockout combined with bruceine D on the proliferation, migration, and invasion of breast cancer MDA-MB-231 cells. Methods Cell Counting Kit-8, wound healing, and Transwell invasion assay were used to detect the effects of bruceine D and siKCNQ1OT1 on...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2023-11-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.23.0456 |
| _version_ | 1797401539981082624 |
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| author | LONG Feng ZHAO Yu HUANG Yong LIU Xiaoyan ZHOU Xuan LI Xue YE Hailin |
| author_facet | LONG Feng ZHAO Yu HUANG Yong LIU Xiaoyan ZHOU Xuan LI Xue YE Hailin |
| author_sort | LONG Feng |
| collection | DOAJ |
| description | Objective To explore the effect of KCNQ1OT1 gene knockout combined with bruceine D on the proliferation, migration, and invasion of breast cancer MDA-MB-231 cells. Methods Cell Counting Kit-8, wound healing, and Transwell invasion assay were used to detect the effects of bruceine D and siKCNQ1OT1 on the viability, migration, and invasion of MDA-MB-231 cells. Effect of bruceine D and siKCNQ1OT1 on the expression of KCNQ1OT1 in MDA-MB-231 cells was detected by qRT-PCR. Western blot was used to detect the effect of bruceine D and siKCNQ1OT1 on the expression of EMT-related proteins and CDC42, p-MKK7, MKK7 proteins in MDA-MB-231 cells. Results Bruceine D and siKCNQ1OT1 could significantly inhibit the viability, migration, and invasion of MDA-MB-231 cells, and the inhibitory effect was enhanced when they were combined (all P < 0.05); bruceine D downregulated the expression of KCNQ1OT1 in MDA-MB-231 cells (all P < 0.05); bruceine D combined with siKCNQ1OT1 significantly decreased CDC42, p-MKK7, N-cadherin, and Vimentin expression in MDA-MB-231 cells and increased the expression of E-cadherin (all P < 0.05). Conclusion Bruceine D combined with siKCNQ1OT1 significantly inhibit the proliferation, migration, invasion, and EMT of human breast cancer MDA-MB-231 cells, and its molecular mechanism may be related to the blocking of CDC42/MKK7 signaling pathway. |
| first_indexed | 2024-03-09T02:12:32Z |
| format | Article |
| id | doaj.art-430bd53726b446dd94bec66b34f61b92 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-03-09T02:12:32Z |
| publishDate | 2023-11-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-430bd53726b446dd94bec66b34f61b922023-12-07T07:46:12ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782023-11-0150111066107410.3971/j.issn.1000-8578.2023.23.04568578.2023.23.0456Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 CellsLONG Feng0ZHAO Yu1HUANG Yong2LIU Xiaoyan3ZHOU Xuan4LI Xue5YE Hailin6School of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, ChinaSchool of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, ChinaSchool of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, ChinaSchool of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, ChinaSchool of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, ChinaSchool of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, ChinaSchool of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, ChinaObjective To explore the effect of KCNQ1OT1 gene knockout combined with bruceine D on the proliferation, migration, and invasion of breast cancer MDA-MB-231 cells. Methods Cell Counting Kit-8, wound healing, and Transwell invasion assay were used to detect the effects of bruceine D and siKCNQ1OT1 on the viability, migration, and invasion of MDA-MB-231 cells. Effect of bruceine D and siKCNQ1OT1 on the expression of KCNQ1OT1 in MDA-MB-231 cells was detected by qRT-PCR. Western blot was used to detect the effect of bruceine D and siKCNQ1OT1 on the expression of EMT-related proteins and CDC42, p-MKK7, MKK7 proteins in MDA-MB-231 cells. Results Bruceine D and siKCNQ1OT1 could significantly inhibit the viability, migration, and invasion of MDA-MB-231 cells, and the inhibitory effect was enhanced when they were combined (all P < 0.05); bruceine D downregulated the expression of KCNQ1OT1 in MDA-MB-231 cells (all P < 0.05); bruceine D combined with siKCNQ1OT1 significantly decreased CDC42, p-MKK7, N-cadherin, and Vimentin expression in MDA-MB-231 cells and increased the expression of E-cadherin (all P < 0.05). Conclusion Bruceine D combined with siKCNQ1OT1 significantly inhibit the proliferation, migration, invasion, and EMT of human breast cancer MDA-MB-231 cells, and its molecular mechanism may be related to the blocking of CDC42/MKK7 signaling pathway.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.23.0456breast cancerbruceine dkcnq1ot1migrationinvasion |
| spellingShingle | LONG Feng ZHAO Yu HUANG Yong LIU Xiaoyan ZHOU Xuan LI Xue YE Hailin Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells Zhongliu Fangzhi Yanjiu breast cancer bruceine d kcnq1ot1 migration invasion |
| title | Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells |
| title_full | Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells |
| title_fullStr | Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells |
| title_full_unstemmed | Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells |
| title_short | Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells |
| title_sort | effects of kcnq1ot1 gene knockout combined with bruceine d on proliferation migration and invasion of breast cancer mda mb 231 cells |
| topic | breast cancer bruceine d kcnq1ot1 migration invasion |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.23.0456 |
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