Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells
Vitamin D is a nutrient and a hormone with multiple effects on immune regulation and respiratory viral infections, which can worsen asthma and lead to severe asthma exacerbations. We set up a complete experimental and analytical pipeline for ATAC-Seq and RNA-Seq to study genome-wide epigenetic chang...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-09-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2019.1622993 |
| _version_ | 1797678879992709120 |
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| author | Nadia Boutaoui Sandeep Puranik Rong Zhang Ting Wang Daniel H. Hui John Brehm Erick Forno Wei Chen Juan C. Celedón |
| author_facet | Nadia Boutaoui Sandeep Puranik Rong Zhang Ting Wang Daniel H. Hui John Brehm Erick Forno Wei Chen Juan C. Celedón |
| author_sort | Nadia Boutaoui |
| collection | DOAJ |
| description | Vitamin D is a nutrient and a hormone with multiple effects on immune regulation and respiratory viral infections, which can worsen asthma and lead to severe asthma exacerbations. We set up a complete experimental and analytical pipeline for ATAC-Seq and RNA-Seq to study genome-wide epigenetic changes in human bronchial epithelial cells of asthmatic subjects, following treatment of these cells with calcitriol (vitamin D3) and Poly (I:C)(a viral analogue). This approach led to the identification of biologically plausible candidate genes for viral infections and asthma, such as DUSP10 and SLC44A1. |
| first_indexed | 2024-03-11T23:06:21Z |
| format | Article |
| id | doaj.art-430c1824604e4db1b8f96d01d9177deb |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:06:21Z |
| publishDate | 2019-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-430c1824604e4db1b8f96d01d9177deb2023-09-21T13:09:22ZengTaylor & Francis GroupEpigenetics1559-22941559-23082019-09-0114984484910.1080/15592294.2019.16229931622993Epigenome-wide effects of vitamin D on asthma bronchial epithelial cellsNadia Boutaoui0Sandeep Puranik1Rong Zhang2Ting Wang3Daniel H. Hui4John Brehm5Erick Forno6Wei Chen7Juan C. Celedón8University of PittsburghUniversity of PittsburghUniversity of PittsburghFred Hutchinson Cancer Research CenterHarvard Medical School, Broad InstituteUniversity of Pittsburgh Medical Center- St. Margret’s Hospital, University of PittsburghUniversity of PittsburghUniversity of PittsburghUniversity of PittsburghVitamin D is a nutrient and a hormone with multiple effects on immune regulation and respiratory viral infections, which can worsen asthma and lead to severe asthma exacerbations. We set up a complete experimental and analytical pipeline for ATAC-Seq and RNA-Seq to study genome-wide epigenetic changes in human bronchial epithelial cells of asthmatic subjects, following treatment of these cells with calcitriol (vitamin D3) and Poly (I:C)(a viral analogue). This approach led to the identification of biologically plausible candidate genes for viral infections and asthma, such as DUSP10 and SLC44A1.http://dx.doi.org/10.1080/15592294.2019.1622993vitamin datac-seqrna-seqasthma |
| spellingShingle | Nadia Boutaoui Sandeep Puranik Rong Zhang Ting Wang Daniel H. Hui John Brehm Erick Forno Wei Chen Juan C. Celedón Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells Epigenetics vitamin d atac-seq rna-seq asthma |
| title | Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells |
| title_full | Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells |
| title_fullStr | Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells |
| title_full_unstemmed | Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells |
| title_short | Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells |
| title_sort | epigenome wide effects of vitamin d on asthma bronchial epithelial cells |
| topic | vitamin d atac-seq rna-seq asthma |
| url | http://dx.doi.org/10.1080/15592294.2019.1622993 |
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