Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS
The projected rise in global ambient temperature by 3–5 °C by the end of this century, along with unpredicted heat waves during critical crop growth stages, can drastically reduce grain yield and will pose a great food security challenge. It is therefore important to identify wheat genetic resources...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/8/1610 |
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| author | Ambreen Mehvish Abdul Aziz Birra Bukhari Humaira Qayyum Zahid Mahmood Muhammad Baber Muhammad Sajjad Xuequn Pang Fenglan Wang |
| author_facet | Ambreen Mehvish Abdul Aziz Birra Bukhari Humaira Qayyum Zahid Mahmood Muhammad Baber Muhammad Sajjad Xuequn Pang Fenglan Wang |
| author_sort | Ambreen Mehvish |
| collection | DOAJ |
| description | The projected rise in global ambient temperature by 3–5 °C by the end of this century, along with unpredicted heat waves during critical crop growth stages, can drastically reduce grain yield and will pose a great food security challenge. It is therefore important to identify wheat genetic resources able to withstand high temperatures, discover genes underpinning resilience to higher temperatures, and deploy such genetic resources in wheat breeding to develop heat-tolerant cultivars. In this study, 180 accessions of synthetic hexaploid wheats (SHWs) were evaluated under normal and late wheat growing seasons (to expose them to higher temperatures) at three locations (Islamabad, Bahawalpur, and Tando Jam), and data were collected on 11 morphological and yield-related traits. The diversity panel was genotyped with a 50 K SNP array to conduct genome-wide association studies (GWASs) for heat tolerance in SHW. A known heat-tolerance locus, <i>TaHST1</i>, was profiled to identify different haplotypes of this locus in SHWs and their association with grain yield and related traits in SHWs. There was a 36% decrease in grain yield (GY), a 23% decrease in thousand-grain weight (TKW), and an 18% decrease in grains per spike (GpS) across three locations in the population due to the heat stress conditions. GWASs identified 143 quantitative trait nucleotides (QTNs) distributed over all 21 chromosomes in the SHWs. Out of these, 52 QTNs were associated with morphological and yield-related traits under heat stress, while 15 of them were pleiotropically associated with multiple traits. The heat shock protein (HSP) framework of the wheat genome was then aligned with the QTNs identified in this study. Seventeen QTNs were in proximity to HSPs on chr2B, chr3D, chr5A, chr5B, chr6D, and chr7D. It is likely that QTNs on the D genome and those in proximity to HSPs may carry novel alleles for heat-tolerance genes. The analysis of <i>TaHST1</i> indicated that 15 haplotypes were present in the SHWs for this locus, while hap1 showed the highest frequency of 25% (33 SHWs). These haplotypes were significantly associated with yield-related traits in the SHWs. New alleles associated with yield-related traits in SHWs could be an excellent reservoir for breeding deployment. |
| first_indexed | 2024-03-11T04:37:08Z |
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| spelling | doaj.art-430fc68cd0d641f9b4a78063d10ef1b22023-11-17T20:59:07ZengMDPI AGPlants2223-77472023-04-01128161010.3390/plants12081610Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWASAmbreen Mehvish0Abdul Aziz1Birra Bukhari2Humaira Qayyum3Zahid Mahmood4Muhammad Baber5Muhammad Sajjad6Xuequn Pang7Fenglan Wang8Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan 60800, PakistanInternational Maize and Wheat Improvement Center (CIMMYT) Pakistan Office, National Agriculture Research Center (NARC), Park Road, Islamabad 44000, PakistanInstitute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanInstitute of Crop Sciences, National Agriculture Research Center (NARC), Park Road, Islamabad 44000, PakistanInstitute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Biosciences, Comsats University, Islamabad 45550, PakistanCollege of Life Sciences, South China Agricultural University, Guangzhou 510642, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou 510642, ChinaThe projected rise in global ambient temperature by 3–5 °C by the end of this century, along with unpredicted heat waves during critical crop growth stages, can drastically reduce grain yield and will pose a great food security challenge. It is therefore important to identify wheat genetic resources able to withstand high temperatures, discover genes underpinning resilience to higher temperatures, and deploy such genetic resources in wheat breeding to develop heat-tolerant cultivars. In this study, 180 accessions of synthetic hexaploid wheats (SHWs) were evaluated under normal and late wheat growing seasons (to expose them to higher temperatures) at three locations (Islamabad, Bahawalpur, and Tando Jam), and data were collected on 11 morphological and yield-related traits. The diversity panel was genotyped with a 50 K SNP array to conduct genome-wide association studies (GWASs) for heat tolerance in SHW. A known heat-tolerance locus, <i>TaHST1</i>, was profiled to identify different haplotypes of this locus in SHWs and their association with grain yield and related traits in SHWs. There was a 36% decrease in grain yield (GY), a 23% decrease in thousand-grain weight (TKW), and an 18% decrease in grains per spike (GpS) across three locations in the population due to the heat stress conditions. GWASs identified 143 quantitative trait nucleotides (QTNs) distributed over all 21 chromosomes in the SHWs. Out of these, 52 QTNs were associated with morphological and yield-related traits under heat stress, while 15 of them were pleiotropically associated with multiple traits. The heat shock protein (HSP) framework of the wheat genome was then aligned with the QTNs identified in this study. Seventeen QTNs were in proximity to HSPs on chr2B, chr3D, chr5A, chr5B, chr6D, and chr7D. It is likely that QTNs on the D genome and those in proximity to HSPs may carry novel alleles for heat-tolerance genes. The analysis of <i>TaHST1</i> indicated that 15 haplotypes were present in the SHWs for this locus, while hap1 showed the highest frequency of 25% (33 SHWs). These haplotypes were significantly associated with yield-related traits in the SHWs. New alleles associated with yield-related traits in SHWs could be an excellent reservoir for breeding deployment.https://www.mdpi.com/2223-7747/12/8/1610synthetic hexaploid wheatassociation mappingheat toleranceheat shock proteinsquantitative trait nucleotides |
| spellingShingle | Ambreen Mehvish Abdul Aziz Birra Bukhari Humaira Qayyum Zahid Mahmood Muhammad Baber Muhammad Sajjad Xuequn Pang Fenglan Wang Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS Plants synthetic hexaploid wheat association mapping heat tolerance heat shock proteins quantitative trait nucleotides |
| title | Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS |
| title_full | Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS |
| title_fullStr | Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS |
| title_full_unstemmed | Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS |
| title_short | Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS |
| title_sort | identification of single nucleotide polymorphisms snps associated with heat tolerance at the reproductive stage in synthetic hexaploid wheats using gwas |
| topic | synthetic hexaploid wheat association mapping heat tolerance heat shock proteins quantitative trait nucleotides |
| url | https://www.mdpi.com/2223-7747/12/8/1610 |
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