Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo development
Abstract Understanding of the distribution of chemerin and its receptors, Chemokine-like Receptor 1 (CMKLR1), G Protein-coupled Receptor 1 (GPR1) and Chemokine (C–C motif) receptor-like 2 (CCRL2), in the egg and the embryonic annexes is currently lacking, and their role during embryogenesis remains...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-12961-4 |
| _version_ | 1828223149323321344 |
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| author | Anthony Estienne Adeline Brossaud Christelle Ramé Ophélie Bernardi Maxime Reverchon Christophe Rat Joël Delaveau Emilie Chambellon Emmanuelle Helloin Pascal Froment Joëlle Dupont |
| author_facet | Anthony Estienne Adeline Brossaud Christelle Ramé Ophélie Bernardi Maxime Reverchon Christophe Rat Joël Delaveau Emilie Chambellon Emmanuelle Helloin Pascal Froment Joëlle Dupont |
| author_sort | Anthony Estienne |
| collection | DOAJ |
| description | Abstract Understanding of the distribution of chemerin and its receptors, Chemokine-like Receptor 1 (CMKLR1), G Protein-coupled Receptor 1 (GPR1) and Chemokine (C–C motif) receptor-like 2 (CCRL2), in the egg and the embryonic annexes is currently lacking, and their role during embryogenesis remains unknown. By immunoblot using monoclonal anti-chicken antibodies and Enzyme Linked Immunosorbent Assays (ELISA), we found that chemerin is expressed 10 times higher in albumen eggs than in blood plasma, and it is also abundant in the perivitelline membrane but undetectable in yolk. Chicken chemerin can inhibit bacterial growth. By Reverse Transcription—quantitative Polymerisation Chain Reaction (RT-qPCR), western-blot, and immunofluorescence, we show that chemerin is locally produced by the oviduct magnum that participates in albumen formation. Using cultures of magnum explants, we demonstrate that progesterone (P4) and oestradiol (E2) treatment increases chemerin secretion into cultured media and expression in magnum. Chemerin and its three receptors are present in amniotic and Chorio Allantoic Membranes (CAM). Only CMKLR1 expression decreased from embryonic day (ED) 7 to ED11 and remained low until ED18. Chemerin concentrations strongly increased in amniotic fluid at D14 when egg albumen crossed the amniotic membrane. In ovo injections of neutralising chemerin and CMKLR1 antibodies (0.01, 0.1 and 1 µg) increased embryo mortality, which occurred mainly at ED12-13, in a dose-dependent manner. Chemerin treatment increased primary CAM viability. Finally, chemerin and CMKLR1 inhibition within the CAM led to a decrease in blood vessel development and associated angiogenic gene expression. Our results show an important function of the chemerin system during embryo development in chickens, suggesting the potential use of this adipokine as a predictive marker for egg fertility or hatchability. |
| first_indexed | 2024-04-12T17:03:18Z |
| format | Article |
| id | doaj.art-4311ed132bd848628fe6bdb9ddb9e7fe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T17:03:18Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4311ed132bd848628fe6bdb9ddb9e7fe2022-12-22T03:24:00ZengNature PortfolioScientific Reports2045-23222022-05-0112111710.1038/s41598-022-12961-4Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo developmentAnthony Estienne0Adeline Brossaud1Christelle Ramé2Ophélie Bernardi3Maxime Reverchon4Christophe Rat5Joël Delaveau6Emilie Chambellon7Emmanuelle Helloin8Pascal Froment9Joëlle Dupont10Centre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, INRAE, Université de Tours, Physiologie de la Reproduction et des Comportements, UMR85Centre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, INRAE, Université de Tours, Physiologie de la Reproduction et des Comportements, UMR85Centre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, INRAE, Université de Tours, Physiologie de la Reproduction et des Comportements, UMR85Centre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, INRAE, Université de Tours, Physiologie de la Reproduction et des Comportements, UMR85SYSAAF-Syndicat des Sélectionneurs Avicoles et Aquacoles Français, Centre INRA Val de LoireInstitut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement-Unité Expérimentale du Pôle d’Expérimentation Avicole de Tours UEPEAT 1295Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement-Unité Expérimentale du Pôle d’Expérimentation Avicole de Tours UEPEAT 1295Institut National de Recherche Pour l’Agriculture, l’Alimentation et l’Environnement-Unité Infectiologie et Santé Publique, Université de ToursInstitut National de Recherche Pour l’Agriculture, l’Alimentation et l’Environnement-Unité Infectiologie et Santé Publique, Université de ToursCentre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, INRAE, Université de Tours, Physiologie de la Reproduction et des Comportements, UMR85Centre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, INRAE, Université de Tours, Physiologie de la Reproduction et des Comportements, UMR85Abstract Understanding of the distribution of chemerin and its receptors, Chemokine-like Receptor 1 (CMKLR1), G Protein-coupled Receptor 1 (GPR1) and Chemokine (C–C motif) receptor-like 2 (CCRL2), in the egg and the embryonic annexes is currently lacking, and their role during embryogenesis remains unknown. By immunoblot using monoclonal anti-chicken antibodies and Enzyme Linked Immunosorbent Assays (ELISA), we found that chemerin is expressed 10 times higher in albumen eggs than in blood plasma, and it is also abundant in the perivitelline membrane but undetectable in yolk. Chicken chemerin can inhibit bacterial growth. By Reverse Transcription—quantitative Polymerisation Chain Reaction (RT-qPCR), western-blot, and immunofluorescence, we show that chemerin is locally produced by the oviduct magnum that participates in albumen formation. Using cultures of magnum explants, we demonstrate that progesterone (P4) and oestradiol (E2) treatment increases chemerin secretion into cultured media and expression in magnum. Chemerin and its three receptors are present in amniotic and Chorio Allantoic Membranes (CAM). Only CMKLR1 expression decreased from embryonic day (ED) 7 to ED11 and remained low until ED18. Chemerin concentrations strongly increased in amniotic fluid at D14 when egg albumen crossed the amniotic membrane. In ovo injections of neutralising chemerin and CMKLR1 antibodies (0.01, 0.1 and 1 µg) increased embryo mortality, which occurred mainly at ED12-13, in a dose-dependent manner. Chemerin treatment increased primary CAM viability. Finally, chemerin and CMKLR1 inhibition within the CAM led to a decrease in blood vessel development and associated angiogenic gene expression. Our results show an important function of the chemerin system during embryo development in chickens, suggesting the potential use of this adipokine as a predictive marker for egg fertility or hatchability.https://doi.org/10.1038/s41598-022-12961-4 |
| spellingShingle | Anthony Estienne Adeline Brossaud Christelle Ramé Ophélie Bernardi Maxime Reverchon Christophe Rat Joël Delaveau Emilie Chambellon Emmanuelle Helloin Pascal Froment Joëlle Dupont Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo development Scientific Reports |
| title | Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo development |
| title_full | Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo development |
| title_fullStr | Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo development |
| title_full_unstemmed | Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo development |
| title_short | Chemerin is secreted by the chicken oviduct, accumulates in egg albumen and could promote embryo development |
| title_sort | chemerin is secreted by the chicken oviduct accumulates in egg albumen and could promote embryo development |
| url | https://doi.org/10.1038/s41598-022-12961-4 |
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