Engineering and application of a biosensor with focused ligand specificity
Transcriptional biosensors represent powerful tools for the screening of vast strain libraries, but the broad ligand specificity of some transcriptional regulators (TRs) can prohibit such applications. Here authors present the engineering of a LysG-based biosensor with a focused ligand specificity t...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-18400-0 |
| _version_ | 1818683143207518208 |
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| author | Dennis Della Corte Hugo L. van Beek Falk Syberg Marcus Schallmey Felix Tobola Kai U. Cormann Christine Schlicker Philipp T. Baumann Karin Krumbach Sascha Sokolowsky Connor J. Morris Alexander Grünberger Eckhard Hofmann Gunnar F. Schröder Jan Marienhagen |
| author_facet | Dennis Della Corte Hugo L. van Beek Falk Syberg Marcus Schallmey Felix Tobola Kai U. Cormann Christine Schlicker Philipp T. Baumann Karin Krumbach Sascha Sokolowsky Connor J. Morris Alexander Grünberger Eckhard Hofmann Gunnar F. Schröder Jan Marienhagen |
| author_sort | Dennis Della Corte |
| collection | DOAJ |
| description | Transcriptional biosensors represent powerful tools for the screening of vast strain libraries, but the broad ligand specificity of some transcriptional regulators (TRs) can prohibit such applications. Here authors present the engineering of a LysG-based biosensor with a focused ligand specificity to isolate L-histidine-producing strains. |
| first_indexed | 2024-12-17T10:30:03Z |
| format | Article |
| id | doaj.art-43140052d6614d1abc93af4f2bc79843 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T10:30:03Z |
| publishDate | 2020-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-43140052d6614d1abc93af4f2bc798432022-12-21T21:52:33ZengNature PortfolioNature Communications2041-17232020-09-0111111110.1038/s41467-020-18400-0Engineering and application of a biosensor with focused ligand specificityDennis Della Corte0Hugo L. van Beek1Falk Syberg2Marcus Schallmey3Felix Tobola4Kai U. Cormann5Christine Schlicker6Philipp T. Baumann7Karin Krumbach8Sascha Sokolowsky9Connor J. Morris10Alexander Grünberger11Eckhard Hofmann12Gunnar F. Schröder13Jan Marienhagen14Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum JülichInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichProtein Crystallography, Biophysics, Ruhr University BochumInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichProtein Crystallography, Biophysics, Ruhr University BochumInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichDepartment of Physics & Astronomy, Brigham Young UniversityInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichProtein Crystallography, Biophysics, Ruhr University BochumInstitute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum JülichInstitute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum JülichTranscriptional biosensors represent powerful tools for the screening of vast strain libraries, but the broad ligand specificity of some transcriptional regulators (TRs) can prohibit such applications. Here authors present the engineering of a LysG-based biosensor with a focused ligand specificity to isolate L-histidine-producing strains.https://doi.org/10.1038/s41467-020-18400-0 |
| spellingShingle | Dennis Della Corte Hugo L. van Beek Falk Syberg Marcus Schallmey Felix Tobola Kai U. Cormann Christine Schlicker Philipp T. Baumann Karin Krumbach Sascha Sokolowsky Connor J. Morris Alexander Grünberger Eckhard Hofmann Gunnar F. Schröder Jan Marienhagen Engineering and application of a biosensor with focused ligand specificity Nature Communications |
| title | Engineering and application of a biosensor with focused ligand specificity |
| title_full | Engineering and application of a biosensor with focused ligand specificity |
| title_fullStr | Engineering and application of a biosensor with focused ligand specificity |
| title_full_unstemmed | Engineering and application of a biosensor with focused ligand specificity |
| title_short | Engineering and application of a biosensor with focused ligand specificity |
| title_sort | engineering and application of a biosensor with focused ligand specificity |
| url | https://doi.org/10.1038/s41467-020-18400-0 |
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