Role of miR-100-5p and CDC25A in breast carcinoma cells
Objective To inquiry about mechanism of miR-100-5p/CDC25A axis in breast carcinoma (BC), thus offering a new direction for BC targeted treatment. Methods qRT-PCR was employed to explore miR-100-5p and CDC25A mRNA levels. Western blot was employed for detecting protein expression of CDC25A. Targeting...
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| Format: | Article |
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PeerJ Inc.
2022-01-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/12263.pdf |
| _version_ | 1797418038223437824 |
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| author | Xiaoping Li Yanli Ren Donghong Liu Xi Yu Keda Chen |
| author_facet | Xiaoping Li Yanli Ren Donghong Liu Xi Yu Keda Chen |
| author_sort | Xiaoping Li |
| collection | DOAJ |
| description | Objective To inquiry about mechanism of miR-100-5p/CDC25A axis in breast carcinoma (BC), thus offering a new direction for BC targeted treatment. Methods qRT-PCR was employed to explore miR-100-5p and CDC25A mRNA levels. Western blot was employed for detecting protein expression of CDC25A. Targeting relationship of miR-100-5p and CDC25A was verified by dual-luciferase assay. In vitro experiments were used for assessment of cell functions. Results In BC tissue and cells, miR-100-5p was significantly lowly expressed (P < 0.05) while CDC25A was highly expressed. Besides, miR-100-5p downregulated CDC25A level. miR-100-5p had a marked influence on the prognosis of patients. The forced miR-100-5p expression hindered BC cell proliferation, migration and invasion, and facilitated cell apoptosis. Upregulated miR-100-5p weakened promotion of CDC25A on BC cell growth. Conclusion Together, these findings unveiled that CDC25A may be a key target of miR-100-5p that mediated progression of BC cells. Hence, miR-100-5p overexpression or CDC25A suppression may contribute to BC diagnosis. |
| first_indexed | 2024-03-09T06:26:46Z |
| format | Article |
| id | doaj.art-43148ceac5b44f48a68bfd616a4bdb19 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:26:46Z |
| publishDate | 2022-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-43148ceac5b44f48a68bfd616a4bdb192023-12-03T11:18:16ZengPeerJ Inc.PeerJ2167-83592022-01-019e1226310.7717/peerj.12263Role of miR-100-5p and CDC25A in breast carcinoma cellsXiaoping Li0Yanli Ren1Donghong Liu2Xi Yu3Keda Chen4Faculty of Medicine, Macau University of Science and Technology, Macau, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang province, ChinaDepartment of Laboratory Medicine, Hangyan hospital of Wenzhou Medical University, Taizhou First People’s Hospital, Taizhou, Zhejiang, ChinaFaculty of Medicine, Macau University of Science and Technology, Macau, ChinaShulan International Medical College, Zhejiang Shuren University, Hangzhou, Zhejiang province, ChinaObjective To inquiry about mechanism of miR-100-5p/CDC25A axis in breast carcinoma (BC), thus offering a new direction for BC targeted treatment. Methods qRT-PCR was employed to explore miR-100-5p and CDC25A mRNA levels. Western blot was employed for detecting protein expression of CDC25A. Targeting relationship of miR-100-5p and CDC25A was verified by dual-luciferase assay. In vitro experiments were used for assessment of cell functions. Results In BC tissue and cells, miR-100-5p was significantly lowly expressed (P < 0.05) while CDC25A was highly expressed. Besides, miR-100-5p downregulated CDC25A level. miR-100-5p had a marked influence on the prognosis of patients. The forced miR-100-5p expression hindered BC cell proliferation, migration and invasion, and facilitated cell apoptosis. Upregulated miR-100-5p weakened promotion of CDC25A on BC cell growth. Conclusion Together, these findings unveiled that CDC25A may be a key target of miR-100-5p that mediated progression of BC cells. Hence, miR-100-5p overexpression or CDC25A suppression may contribute to BC diagnosis.https://peerj.com/articles/12263.pdfMiR-100-5pCDC25ABreast carcinomaProliferationMigration and invasion |
| spellingShingle | Xiaoping Li Yanli Ren Donghong Liu Xi Yu Keda Chen Role of miR-100-5p and CDC25A in breast carcinoma cells PeerJ MiR-100-5p CDC25A Breast carcinoma Proliferation Migration and invasion |
| title | Role of miR-100-5p and CDC25A in breast carcinoma cells |
| title_full | Role of miR-100-5p and CDC25A in breast carcinoma cells |
| title_fullStr | Role of miR-100-5p and CDC25A in breast carcinoma cells |
| title_full_unstemmed | Role of miR-100-5p and CDC25A in breast carcinoma cells |
| title_short | Role of miR-100-5p and CDC25A in breast carcinoma cells |
| title_sort | role of mir 100 5p and cdc25a in breast carcinoma cells |
| topic | MiR-100-5p CDC25A Breast carcinoma Proliferation Migration and invasion |
| url | https://peerj.com/articles/12263.pdf |
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