A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to Xenobiotics
The study of insect adaptation to the defensive metabolites of host plants and various kinds of insecticides in order to acquire resistance is a hot topic in the pest-control field, but the mechanism is still unclear. In our study, we found that a general signal pathway exists in <i>H. armiger...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/16126 |
| _version_ | 1797457191751385088 |
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| author | Lei Zhang Shenglan Lv Mingjian Li Meng Gu Xiwu Gao |
| author_facet | Lei Zhang Shenglan Lv Mingjian Li Meng Gu Xiwu Gao |
| author_sort | Lei Zhang |
| collection | DOAJ |
| description | The study of insect adaptation to the defensive metabolites of host plants and various kinds of insecticides in order to acquire resistance is a hot topic in the pest-control field, but the mechanism is still unclear. In our study, we found that a general signal pathway exists in <i>H. armigera</i> which can regulate multiple P450s, GSTs and UGTs genes to help insects decrease their susceptibility to xenobiotics. Knockdown of <i>HaNrf2</i> and <i>HaAhR</i> expression could significantly increase the toxicity of xenobiotics to <i>H. armigera</i>, and simultaneously decrease the gene expression of P450s, GSTs and UGTs which are related to the xenobiotic metabolism and synthesis of insect hormone pathways. Then, we used EMSA and dual luciferase assay to verify that a crosstalk exists between AhR and Nrf2 to regulate multiple P450s, GSTs and UGTs genes to mediate <i>H. armigera</i> susceptibility to plant allelochemicals and insecticides. The detoxification genes’ expression network which can be regulated by Nrf2 and AhR is still unknown, and there were also no reports about the crosstalk between AhR and Nrf2 that exist in insects and can regulate multiple detoxification genes’ expression. Our results provide a new general signaling pathway to reveal the adaptive mechanism of insects to xenobiotics and provides further insight into designing effective pest-management strategies to avoid the overuse of insecticides. |
| first_indexed | 2024-03-09T16:18:39Z |
| format | Article |
| id | doaj.art-4314bf61a85d4a18a64fb15045926af7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:18:39Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4314bf61a85d4a18a64fb15045926af72023-11-24T15:33:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241612610.3390/ijms232416126A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to XenobioticsLei Zhang0Shenglan Lv1Mingjian Li2Meng Gu3Xiwu Gao4College of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaThe study of insect adaptation to the defensive metabolites of host plants and various kinds of insecticides in order to acquire resistance is a hot topic in the pest-control field, but the mechanism is still unclear. In our study, we found that a general signal pathway exists in <i>H. armigera</i> which can regulate multiple P450s, GSTs and UGTs genes to help insects decrease their susceptibility to xenobiotics. Knockdown of <i>HaNrf2</i> and <i>HaAhR</i> expression could significantly increase the toxicity of xenobiotics to <i>H. armigera</i>, and simultaneously decrease the gene expression of P450s, GSTs and UGTs which are related to the xenobiotic metabolism and synthesis of insect hormone pathways. Then, we used EMSA and dual luciferase assay to verify that a crosstalk exists between AhR and Nrf2 to regulate multiple P450s, GSTs and UGTs genes to mediate <i>H. armigera</i> susceptibility to plant allelochemicals and insecticides. The detoxification genes’ expression network which can be regulated by Nrf2 and AhR is still unknown, and there were also no reports about the crosstalk between AhR and Nrf2 that exist in insects and can regulate multiple detoxification genes’ expression. Our results provide a new general signaling pathway to reveal the adaptive mechanism of insects to xenobiotics and provides further insight into designing effective pest-management strategies to avoid the overuse of insecticides.https://www.mdpi.com/1422-0067/23/24/16126xenobioticsdetoxification genestranscription factorregulation |
| spellingShingle | Lei Zhang Shenglan Lv Mingjian Li Meng Gu Xiwu Gao A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to Xenobiotics International Journal of Molecular Sciences xenobiotics detoxification genes transcription factor regulation |
| title | A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to Xenobiotics |
| title_full | A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to Xenobiotics |
| title_fullStr | A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to Xenobiotics |
| title_full_unstemmed | A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to Xenobiotics |
| title_short | A General Signal Pathway to Regulate Multiple Detoxification Genes Drives the Evolution of <i>Helicoverpa armigera</i> Adaptation to Xenobiotics |
| title_sort | general signal pathway to regulate multiple detoxification genes drives the evolution of i helicoverpa armigera i adaptation to xenobiotics |
| topic | xenobiotics detoxification genes transcription factor regulation |
| url | https://www.mdpi.com/1422-0067/23/24/16126 |
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