Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma
Abstract Background Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa, which has potential for malignant transformation. MicroRNAs play an important role in immunopathogenesis of OLP, and may be used for prediction of its malignant transformation. This study aimed to asse...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-023-03155-z |
| _version_ | 1797789490019827712 |
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| author | Masoumeh Mehdipour Minoo Shahidi Fahimeh Anbari Homa Mirzaei Soudeh Jafari Azam Kholghi Ehsan Lotfi Soheila Manifar Fatemeh Mashhadiabbas |
| author_facet | Masoumeh Mehdipour Minoo Shahidi Fahimeh Anbari Homa Mirzaei Soudeh Jafari Azam Kholghi Ehsan Lotfi Soheila Manifar Fatemeh Mashhadiabbas |
| author_sort | Masoumeh Mehdipour |
| collection | DOAJ |
| description | Abstract Background Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa, which has potential for malignant transformation. MicroRNAs play an important role in immunopathogenesis of OLP, and may be used for prediction of its malignant transformation. This study aimed to assess the salivary level of microRNA-146a and microRNA-155 biomarkers in patients with OLP and oral squamous cell carcinoma (OSCC). Methods In this case-control study, unstimulated saliva samples were collected from 60 patients, including 15 patients with dysplastic OLP, 15 OLP patients without dysplasia, 15 patients with OSCC, and 15 healthy controls according to the Navazesh technique. After RNA extraction, the expression of microRNA-146a and microRNA-155 was quantified by real-time quantitative polymerase chain reaction (RT-qPCR). The data were analyzed by the Kruskal-Wallis and Dunn-Bonferroni tests. Results The difference in expression of microRNA-146a and microRNA-155 among the four groups was significant (P < 0.05). Pairwise comparisons of the groups showed significantly higher expression of microRNA-146a in OLP (P = 0.004) and dysplastic OLP (P = 0.046) patients compared with the control group. Up-regulation of this biomarker in OSCC patients was not significant compared with the control group (P = 0.076). Up-regulation of micro-RNA-155 was only significant in OLP group, compared with the control group (P = 0.009). No other significant differences were found (P > 0.05). Conclusion Considering the altered expression of MicroRNA-146a and microRNA-155 in dysplastic OLP and OSCC, their altered expression may serve as an alarming sign of malignancy. However, further investigations are still required. |
| first_indexed | 2024-03-13T01:51:24Z |
| format | Article |
| id | doaj.art-431633a1b1054522be4ba620e87d2721 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-03-13T01:51:24Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-431633a1b1054522be4ba620e87d27212023-07-02T11:28:31ZengBMCBMC Oral Health1472-68312023-06-012311910.1186/s12903-023-03155-zSalivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinomaMasoumeh Mehdipour0Minoo Shahidi1Fahimeh Anbari2Homa Mirzaei3Soudeh Jafari4Azam Kholghi5Ehsan Lotfi6Soheila Manifar7Fatemeh Mashhadiabbas8Oral Medicine Department, School of Dentistry, Shahid Beheshti University of Medical SciencesHematology and blood banking Department, Faculty of Allied Medicine, Iran University of Medical SciencesOral Medicine Department, School of Dentistry, Shahid Beheshti University of Medical SciencesOral Medicine Department, School of Dentistry, Shahid Beheshti University of Medical SciencesOral Medicine Department, School of Dentistry, Shahid Beheshti University of Medical SciencesDepartment of Medical Biotechnology, Faculty of Allied Medical Science, Iran University of Medical SciencesDepartment of Medical Biotechnology, Faculty of Allied Medical Science, Iran University of Medical SciencesOral Medicine Department, School of Dentistry, Tehran University of Medical SciencesOral Pathology Department, School of Dentistry, Shahid Beheshti University of Medical SciencesAbstract Background Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa, which has potential for malignant transformation. MicroRNAs play an important role in immunopathogenesis of OLP, and may be used for prediction of its malignant transformation. This study aimed to assess the salivary level of microRNA-146a and microRNA-155 biomarkers in patients with OLP and oral squamous cell carcinoma (OSCC). Methods In this case-control study, unstimulated saliva samples were collected from 60 patients, including 15 patients with dysplastic OLP, 15 OLP patients without dysplasia, 15 patients with OSCC, and 15 healthy controls according to the Navazesh technique. After RNA extraction, the expression of microRNA-146a and microRNA-155 was quantified by real-time quantitative polymerase chain reaction (RT-qPCR). The data were analyzed by the Kruskal-Wallis and Dunn-Bonferroni tests. Results The difference in expression of microRNA-146a and microRNA-155 among the four groups was significant (P < 0.05). Pairwise comparisons of the groups showed significantly higher expression of microRNA-146a in OLP (P = 0.004) and dysplastic OLP (P = 0.046) patients compared with the control group. Up-regulation of this biomarker in OSCC patients was not significant compared with the control group (P = 0.076). Up-regulation of micro-RNA-155 was only significant in OLP group, compared with the control group (P = 0.009). No other significant differences were found (P > 0.05). Conclusion Considering the altered expression of MicroRNA-146a and microRNA-155 in dysplastic OLP and OSCC, their altered expression may serve as an alarming sign of malignancy. However, further investigations are still required.https://doi.org/10.1186/s12903-023-03155-zLichen Planus, oralSquamous cell carcinoma of head and neckMicroRNAsSaliva |
| spellingShingle | Masoumeh Mehdipour Minoo Shahidi Fahimeh Anbari Homa Mirzaei Soudeh Jafari Azam Kholghi Ehsan Lotfi Soheila Manifar Fatemeh Mashhadiabbas Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma BMC Oral Health Lichen Planus, oral Squamous cell carcinoma of head and neck MicroRNAs Saliva |
| title | Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma |
| title_full | Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma |
| title_fullStr | Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma |
| title_full_unstemmed | Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma |
| title_short | Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma |
| title_sort | salivary level of microrna 146a and microrna 155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma |
| topic | Lichen Planus, oral Squamous cell carcinoma of head and neck MicroRNAs Saliva |
| url | https://doi.org/10.1186/s12903-023-03155-z |
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