Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriers
Background: Hepatitis B virus (HBV) is a public health threatening virus and is classified into more than eight genotypes and more than forty subgenotypes. Objectives: To characterize and propose novel strains assigned as A8 and D12. Methods: Four out of 133 HBV complete genome sequences, isolated f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971220300515 |
| _version_ | 1818487332656906240 |
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| author | Marijn Thijssen Nídia Sequeira Trovão Thomas Mina Piet Maes Mahmoud Reza Pourkarim |
| author_facet | Marijn Thijssen Nídia Sequeira Trovão Thomas Mina Piet Maes Mahmoud Reza Pourkarim |
| author_sort | Marijn Thijssen |
| collection | DOAJ |
| description | Background: Hepatitis B virus (HBV) is a public health threatening virus and is classified into more than eight genotypes and more than forty subgenotypes. Objectives: To characterize and propose novel strains assigned as A8 and D12. Methods: Four out of 133 HBV complete genome sequences, isolated from Belgian chronic carriers with African origin were phylogenetically analyzed. Results: Phylogenetic analyses of HBV genotypes A and D strains exhibited separate clusters supported by significant bootstrap values. The two genotype A strains isolated from Congolese patients, and two genotype D strains isolated from Ghanaian carriers clustered separately from the other known subgenotypes A (A1–A6 and quasi-subgenotypes) and subgenotypes D (D1–D11). The mean inter-subgenotypic nucleotide divergence over the full-length genome sequence between the novel strains (A8 and D12) and A1–A7 and D1–D11 subgenotypes was higher than 4%. Conclusions: Phylogenetic analysis of the full-length HBV genome sequences revealed a novel subgenotype and quasi-subgenotype based on the nucleotide divergence and identification of novel amino acids motifs in different ORFs. We identified two strains of the novel subgenotype A8 and two strains of the novel quasi-subgenotype D12. Notably, the analysis demonstrated that the subgenotype A8 strains are a basal lineage that diverged before the other African subgenotypes A. Keywords: Novel, Subgenotype, HBV, Africa, Classification, Evolution |
| first_indexed | 2024-12-10T16:36:26Z |
| format | Article |
| id | doaj.art-431652f1a02d4d51b8fde399705237d8 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-10T16:36:26Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-431652f1a02d4d51b8fde399705237d82022-12-22T01:41:23ZengElsevierInternational Journal of Infectious Diseases1201-97122020-04-019398101Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriersMarijn Thijssen0Nídia Sequeira Trovão1Thomas Mina2Piet Maes3Mahmoud Reza Pourkarim4KU Leuven, Department of Microbiology, Immunology and Transplantation, Division of Clinical and Epidemiological Virology, BE3000 Leuven, BelgiumKU Leuven, Department of Microbiology, Immunology and Transplantation, Division of Clinical and Epidemiological Virology, BE3000 Leuven, BelgiumMina Clinical Laboratory, Gregori Afxentiou, Iocasti Court Block A, Flat 22 Mesa Yitonia, 4003 Lemesos, CyprusKU Leuven, Department of Microbiology, Immunology and Transplantation, Division of Clinical and Epidemiological Virology, BE3000 Leuven, BelgiumKU Leuven, Department of Microbiology, Immunology and Transplantation, Division of Clinical and Epidemiological Virology, BE3000 Leuven, Belgium; Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran; Blood Transfusion Research Centre, High Institute for Research and Education in Transfusion Medicine, Hemmat Exp Way, 14665-1157 Tehran, Iran; Corresponding author at: Division of Clinical and Epidemiological Virology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Herestraat 49, Box 1040, BE3000 Leuven, Belgium.Background: Hepatitis B virus (HBV) is a public health threatening virus and is classified into more than eight genotypes and more than forty subgenotypes. Objectives: To characterize and propose novel strains assigned as A8 and D12. Methods: Four out of 133 HBV complete genome sequences, isolated from Belgian chronic carriers with African origin were phylogenetically analyzed. Results: Phylogenetic analyses of HBV genotypes A and D strains exhibited separate clusters supported by significant bootstrap values. The two genotype A strains isolated from Congolese patients, and two genotype D strains isolated from Ghanaian carriers clustered separately from the other known subgenotypes A (A1–A6 and quasi-subgenotypes) and subgenotypes D (D1–D11). The mean inter-subgenotypic nucleotide divergence over the full-length genome sequence between the novel strains (A8 and D12) and A1–A7 and D1–D11 subgenotypes was higher than 4%. Conclusions: Phylogenetic analysis of the full-length HBV genome sequences revealed a novel subgenotype and quasi-subgenotype based on the nucleotide divergence and identification of novel amino acids motifs in different ORFs. We identified two strains of the novel subgenotype A8 and two strains of the novel quasi-subgenotype D12. Notably, the analysis demonstrated that the subgenotype A8 strains are a basal lineage that diverged before the other African subgenotypes A. Keywords: Novel, Subgenotype, HBV, Africa, Classification, Evolutionhttp://www.sciencedirect.com/science/article/pii/S1201971220300515 |
| spellingShingle | Marijn Thijssen Nídia Sequeira Trovão Thomas Mina Piet Maes Mahmoud Reza Pourkarim Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriers International Journal of Infectious Diseases |
| title | Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriers |
| title_full | Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriers |
| title_fullStr | Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriers |
| title_full_unstemmed | Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriers |
| title_short | Novel hepatitis B virus subgenotype A8 and quasi-subgenotype D12 in African–Belgian chronic carriers |
| title_sort | novel hepatitis b virus subgenotype a8 and quasi subgenotype d12 in african belgian chronic carriers |
| url | http://www.sciencedirect.com/science/article/pii/S1201971220300515 |
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