Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy
Multi-component drugs (MCDs) can induce various cellular changes covering multiple levels, from molecular and subcellular structure to cell morphology. A “non-invasive” method for comprehensively detecting the dynamic changes of cellular fine structure and chemical components on the subcellular leve...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/16/12750 |
| _version_ | 1797584477552115712 |
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| author | Wenhao Shang Anpei Ye Yu-Kai Tong |
| author_facet | Wenhao Shang Anpei Ye Yu-Kai Tong |
| author_sort | Wenhao Shang |
| collection | DOAJ |
| description | Multi-component drugs (MCDs) can induce various cellular changes covering multiple levels, from molecular and subcellular structure to cell morphology. A “non-invasive” method for comprehensively detecting the dynamic changes of cellular fine structure and chemical components on the subcellular level is highly desirable for MCD studies. In this study, the subcellular dynamic processes of gastric cancer BGC823 cells after treatment with a multi-component drug, Compound Kushen Injection (CKI), were investigated using a homemade, high-resolution, confocal Raman spectroscopy (RS) device combined with bright-field imaging. The Raman spectra of the nucleus, cytoplasm and intracellular vesicles (0.4–1 μm) were collected simultaneously for each cell treated with CKI at different times and doses. The RS measurements showed that CKI decreased the DNA signatures, which the drug is known to inhibit. Meanwhile, the CKI-induced subcellular dynamic changes in the appearance of numerous intracellular vesicles and the deconstruction of cytoplasm components were observed and discussed. The results demonstrated that high-resolution subcellular micro-Raman spectroscopy has potential for detecting fine cellular dynamic variation induced by drugs and the screening of MCDs in cancer therapy. |
| first_indexed | 2024-03-10T23:52:15Z |
| format | Article |
| id | doaj.art-431c7e7d119f487baaf1edaf5ee1f170 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:52:15Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-431c7e7d119f487baaf1edaf5ee1f1702023-11-19T01:28:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161275010.3390/ijms241612750Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman SpectroscopyWenhao Shang0Anpei Ye1Yu-Kai Tong2Key Laboratory for the Physics and Chemistry of Nanodevices, School of Electronics, Peking University, Beijing 100871, ChinaKey Laboratory for the Physics and Chemistry of Nanodevices, School of Electronics, Peking University, Beijing 100871, ChinaKey Laboratory for the Physics and Chemistry of Nanodevices, School of Electronics, Peking University, Beijing 100871, ChinaMulti-component drugs (MCDs) can induce various cellular changes covering multiple levels, from molecular and subcellular structure to cell morphology. A “non-invasive” method for comprehensively detecting the dynamic changes of cellular fine structure and chemical components on the subcellular level is highly desirable for MCD studies. In this study, the subcellular dynamic processes of gastric cancer BGC823 cells after treatment with a multi-component drug, Compound Kushen Injection (CKI), were investigated using a homemade, high-resolution, confocal Raman spectroscopy (RS) device combined with bright-field imaging. The Raman spectra of the nucleus, cytoplasm and intracellular vesicles (0.4–1 μm) were collected simultaneously for each cell treated with CKI at different times and doses. The RS measurements showed that CKI decreased the DNA signatures, which the drug is known to inhibit. Meanwhile, the CKI-induced subcellular dynamic changes in the appearance of numerous intracellular vesicles and the deconstruction of cytoplasm components were observed and discussed. The results demonstrated that high-resolution subcellular micro-Raman spectroscopy has potential for detecting fine cellular dynamic variation induced by drugs and the screening of MCDs in cancer therapy.https://www.mdpi.com/1422-0067/24/16/12750subcellulardynamicdrug CKIRaman spectroscopyintracellular vesicles |
| spellingShingle | Wenhao Shang Anpei Ye Yu-Kai Tong Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy International Journal of Molecular Sciences subcellular dynamic drug CKI Raman spectroscopy intracellular vesicles |
| title | Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy |
| title_full | Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy |
| title_fullStr | Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy |
| title_full_unstemmed | Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy |
| title_short | Sub-Cellular Dynamic Analysis of BGC823 Cells after Treatment with the Multi-Component Drug CKI Using Raman Spectroscopy |
| title_sort | sub cellular dynamic analysis of bgc823 cells after treatment with the multi component drug cki using raman spectroscopy |
| topic | subcellular dynamic drug CKI Raman spectroscopy intracellular vesicles |
| url | https://www.mdpi.com/1422-0067/24/16/12750 |
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