Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugworm
There is technology available for anti-thrombus with earthworms, but the procedure is complex and extracts protein with inferior purity. In order to develop a simplified process with a stronger purity of protease, we investigated the Lumbricus rubellus earthworm and Perinereis linea lugworm. We puri...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-10-01
|
| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235234091930839X |
| _version_ | 1818031908426088448 |
|---|---|
| author | Yuanyuan Dong Young Min Woo Young-Ho Lee Mee Young Ahn Dong-Guen Lee Sang-Hyeon Lee Jong-Myung Ha Chan-Il Park Andre Kim |
| author_facet | Yuanyuan Dong Young Min Woo Young-Ho Lee Mee Young Ahn Dong-Guen Lee Sang-Hyeon Lee Jong-Myung Ha Chan-Il Park Andre Kim |
| author_sort | Yuanyuan Dong |
| collection | DOAJ |
| description | There is technology available for anti-thrombus with earthworms, but the procedure is complex and extracts protein with inferior purity. In order to develop a simplified process with a stronger purity of protease, we investigated the Lumbricus rubellus earthworm and Perinereis linea lugworm. We purified water extracts cut off at 10 kDa of molecular weight using ultrafiltration because proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues. We purified EW1 (raw earthworm extract), EW2 (molecular weight (m.w) > 10 kDa of earthworm extract), and EW3 (m.w < 10 kDa) from the Lumbricus rubellus earthworm. Likewise, we purified LW1 (wild lugworm extract), LW2 (m.w > 10 kDa), and LW3 (m.w < 10 kDa) from the Perinereis linea lugworm. Using a fibrin assay, we found that fibrinolytic activity of the specimens had a rank order of clear zone diameter: EW2 > EW1 > EW3 > LW2 > LW1 > LW3. In particular, EW2 and LW2 showed a potent fibrinolytic effect in two different worm specimens. The protein content of each sample was detected as 2.34 (EW1), 3.03 (EW2), 2.80 (LW1), 3.71 (LW2) mg/ml respectively, and their molecular weights were measured using SDS-PAGE. The samples contained the following amounts of total fatty acids: EW1, 3.61%; EW2, 0.48%; LW1, 4.96%; and LW2, 0.23%.We developed a process to increase the thrombolytic effect with a higher purity protein. The study results demonstrate this procedure and provide basic data for developing an anti-thrombolytic agent. Keywords: Lumbricus rubellus, Perinereis linea, Fibrinolytic effect, Fatty acid |
| first_indexed | 2024-12-10T05:58:57Z |
| format | Article |
| id | doaj.art-431cc6cb78dc41e680a875b4fb95523f |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-10T05:58:57Z |
| publishDate | 2019-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-431cc6cb78dc41e680a875b4fb95523f2022-12-22T01:59:51ZengElsevierData in Brief2352-34092019-10-0126Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugwormYuanyuan Dong0Young Min Woo1Young-Ho Lee2Mee Young Ahn3Dong-Guen Lee4Sang-Hyeon Lee5Jong-Myung Ha6Chan-Il Park7Andre Kim8Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University, 46958, Busan, Republic of KoreaDepartment of Natural Science Institute, Silla University, 46958, Busan, Republic of KoreaProtein Structure Group, Korea Basic Science Institute, 28119, Chungcheongbuk-do, Republic of KoreaDepartment of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University, 46958, Busan, Republic of KoreaDepartment of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University, 46958, Busan, Republic of KoreaDepartment of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University, 46958, Busan, Republic of KoreaDepartment of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University, 46958, Busan, Republic of KoreaInstitute of Marine Industry, College of Marine Science, Gyeongsang National University, 455, Tongyeong, 650-160, Republic of Korea; Corresponding author.Department of Pharmaceutical Engineering, College of Medical and Life Sciences, Silla University, 46958, Busan, Republic of Korea; Corresponding author.There is technology available for anti-thrombus with earthworms, but the procedure is complex and extracts protein with inferior purity. In order to develop a simplified process with a stronger purity of protease, we investigated the Lumbricus rubellus earthworm and Perinereis linea lugworm. We purified water extracts cut off at 10 kDa of molecular weight using ultrafiltration because proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues. We purified EW1 (raw earthworm extract), EW2 (molecular weight (m.w) > 10 kDa of earthworm extract), and EW3 (m.w < 10 kDa) from the Lumbricus rubellus earthworm. Likewise, we purified LW1 (wild lugworm extract), LW2 (m.w > 10 kDa), and LW3 (m.w < 10 kDa) from the Perinereis linea lugworm. Using a fibrin assay, we found that fibrinolytic activity of the specimens had a rank order of clear zone diameter: EW2 > EW1 > EW3 > LW2 > LW1 > LW3. In particular, EW2 and LW2 showed a potent fibrinolytic effect in two different worm specimens. The protein content of each sample was detected as 2.34 (EW1), 3.03 (EW2), 2.80 (LW1), 3.71 (LW2) mg/ml respectively, and their molecular weights were measured using SDS-PAGE. The samples contained the following amounts of total fatty acids: EW1, 3.61%; EW2, 0.48%; LW1, 4.96%; and LW2, 0.23%.We developed a process to increase the thrombolytic effect with a higher purity protein. The study results demonstrate this procedure and provide basic data for developing an anti-thrombolytic agent. Keywords: Lumbricus rubellus, Perinereis linea, Fibrinolytic effect, Fatty acidhttp://www.sciencedirect.com/science/article/pii/S235234091930839X |
| spellingShingle | Yuanyuan Dong Young Min Woo Young-Ho Lee Mee Young Ahn Dong-Guen Lee Sang-Hyeon Lee Jong-Myung Ha Chan-Il Park Andre Kim Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugworm Data in Brief |
| title | Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugworm |
| title_full | Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugworm |
| title_fullStr | Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugworm |
| title_full_unstemmed | Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugworm |
| title_short | Data on the potent fibrinolytic effects of the Lumbricus rubellus earthworm and the Perinereis linea lugworm |
| title_sort | data on the potent fibrinolytic effects of the lumbricus rubellus earthworm and the perinereis linea lugworm |
| url | http://www.sciencedirect.com/science/article/pii/S235234091930839X |
| work_keys_str_mv | AT yuanyuandong dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT youngminwoo dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT youngholee dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT meeyoungahn dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT dongguenlee dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT sanghyeonlee dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT jongmyungha dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT chanilpark dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm AT andrekim dataonthepotentfibrinolyticeffectsofthelumbricusrubellusearthwormandtheperinereislinealugworm |