Ovarian hormones influence immune response to Staphylococcus aureus infection
Objective: Staphylococcus aureus infections remain associated with considerable morbidity and mortality in both hospitals and the community. There is little information regarding the role of ovarian hormones in infections caused by S. aureus. The aim of this study was to evaluate the effects of ovar...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-11-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867020301562 |
| _version_ | 1818435619488006144 |
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| author | Clarissa Leal Silva e Souza Hellen Braga Martins Oliveira Manoel N. Santos Júnior Mariângela de Oliveira Silva Igor Lopes Coqueiro Ícaro Bonyek Santos da Silva Guilherme Barreto Campos Robson Amaro Augusto da Silva Telma de Jesus Soares Márcio Vasconcelos de Oliveira Jorge Timenetsky Lucas Miranda Marques |
| author_facet | Clarissa Leal Silva e Souza Hellen Braga Martins Oliveira Manoel N. Santos Júnior Mariângela de Oliveira Silva Igor Lopes Coqueiro Ícaro Bonyek Santos da Silva Guilherme Barreto Campos Robson Amaro Augusto da Silva Telma de Jesus Soares Márcio Vasconcelos de Oliveira Jorge Timenetsky Lucas Miranda Marques |
| author_sort | Clarissa Leal Silva e Souza |
| collection | DOAJ |
| description | Objective: Staphylococcus aureus infections remain associated with considerable morbidity and mortality in both hospitals and the community. There is little information regarding the role of ovarian hormones in infections caused by S. aureus. The aim of this study was to evaluate the effects of ovariectomy in the immune response induced by S. aureus. Methods: Female mice BALB/c were ovariectomized (OVX) to significantly reduce the level of ovarian hormones. We also used sham-operated animals. The mice were inoculated intraperitoneally with S. aureus. Blood samples were collected for leukocyte count and bacterial quantification. The uterus and spleen were removed and weighed to calculate the uterine and splenic indexes. Lungs were removed and fractionated for immunohistochemical analysis for macrophage detection (anti-CD68) and relative gene expression of IL-6, IL-1β and TNF-α by RT-PCR. Results: Ovariectomy enlarged spleen size and generally increased circulating lymphocytes. OVX females experienced a continuation of the initial reduction of lymphocytes and a monocyte and neutrophil late response compared to shams (p ≥ 0.05). Moreover, OVX females showed neutropenia after 168 h of infection (p ≥ 0.05). Macrophage response in the lungs were less pronounced in OVX females in the initial hours of infection (p ≥ 0.01). OVX females showed a higher relative gene expression of IL-1β, IL-6 and TNF-α in the lung at the beginning of the infection compared to sham females (p ≥ 0.01). Among the uninfected females, the OVX control females showed a higher expression of IL-6 in the lung compared to the sham control females (p ≥ 0.05). In this model, the lack of ovarian hormones caused a minor increase in circulating leukocytes during the initial stage of infection by S. aureus and increased pulmonary gene expression of IL-1β, IL-6, and TNF-α. Ovariectomy alone enlarged the spleen and increased circulating lymphocytes. Ovarian hormones acted as immunoprotectors against S. aureus infection. |
| first_indexed | 2024-12-14T16:55:46Z |
| format | Article |
| id | doaj.art-431e582e4c4747ecbc7690e1047a97b9 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-14T16:55:46Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-431e582e4c4747ecbc7690e1047a97b92022-12-21T22:53:57ZengElsevierBrazilian Journal of Infectious Diseases1413-86702020-11-01246534544Ovarian hormones influence immune response to Staphylococcus aureus infectionClarissa Leal Silva e Souza0Hellen Braga Martins Oliveira1Manoel N. Santos Júnior2Mariângela de Oliveira Silva3Igor Lopes Coqueiro4Ícaro Bonyek Santos da Silva5Guilherme Barreto Campos6Robson Amaro Augusto da Silva7Telma de Jesus Soares8Márcio Vasconcelos de Oliveira9Jorge Timenetsky10Lucas Miranda Marques11Universidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, Brazil; Universidade Estadual de Santa Cruz, Bahia, BA, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, Brazil; Universidade Estadual de Santa Cruz, Bahia, BA, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, Brazil; Universidade de São Paulo, Brasil, Instituto de Ciências Biomédicas, São Paulo, SP, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, Brazil; Fundação Oswaldo Cruz, Instituto Gonçalo Muniz, Salvador, BA, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, Brazil; Universidade de São Paulo, Brasil, Instituto de Ciências Biomédicas, São Paulo, SP, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, BrazilUniversidade de São Paulo, Brasil, Instituto de Ciências Biomédicas, São Paulo, SP, BrazilUniversidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, Brazil; Universidade Estadual de Santa Cruz, Bahia, BA, Brazil; Universidade de São Paulo, Brasil, Instituto de Ciências Biomédicas, São Paulo, SP, Brazil; Corresponding author at: Universidade Federal da Bahia, Instituto Multidisciplinar em Saúde, BA, Brazil.Objective: Staphylococcus aureus infections remain associated with considerable morbidity and mortality in both hospitals and the community. There is little information regarding the role of ovarian hormones in infections caused by S. aureus. The aim of this study was to evaluate the effects of ovariectomy in the immune response induced by S. aureus. Methods: Female mice BALB/c were ovariectomized (OVX) to significantly reduce the level of ovarian hormones. We also used sham-operated animals. The mice were inoculated intraperitoneally with S. aureus. Blood samples were collected for leukocyte count and bacterial quantification. The uterus and spleen were removed and weighed to calculate the uterine and splenic indexes. Lungs were removed and fractionated for immunohistochemical analysis for macrophage detection (anti-CD68) and relative gene expression of IL-6, IL-1β and TNF-α by RT-PCR. Results: Ovariectomy enlarged spleen size and generally increased circulating lymphocytes. OVX females experienced a continuation of the initial reduction of lymphocytes and a monocyte and neutrophil late response compared to shams (p ≥ 0.05). Moreover, OVX females showed neutropenia after 168 h of infection (p ≥ 0.05). Macrophage response in the lungs were less pronounced in OVX females in the initial hours of infection (p ≥ 0.01). OVX females showed a higher relative gene expression of IL-1β, IL-6 and TNF-α in the lung at the beginning of the infection compared to sham females (p ≥ 0.01). Among the uninfected females, the OVX control females showed a higher expression of IL-6 in the lung compared to the sham control females (p ≥ 0.05). In this model, the lack of ovarian hormones caused a minor increase in circulating leukocytes during the initial stage of infection by S. aureus and increased pulmonary gene expression of IL-1β, IL-6, and TNF-α. Ovariectomy alone enlarged the spleen and increased circulating lymphocytes. Ovarian hormones acted as immunoprotectors against S. aureus infection.http://www.sciencedirect.com/science/article/pii/S1413867020301562Staphylococcus aureusOvariectomyPro-inflammatory cytokinesOvarian hormones |
| spellingShingle | Clarissa Leal Silva e Souza Hellen Braga Martins Oliveira Manoel N. Santos Júnior Mariângela de Oliveira Silva Igor Lopes Coqueiro Ícaro Bonyek Santos da Silva Guilherme Barreto Campos Robson Amaro Augusto da Silva Telma de Jesus Soares Márcio Vasconcelos de Oliveira Jorge Timenetsky Lucas Miranda Marques Ovarian hormones influence immune response to Staphylococcus aureus infection Brazilian Journal of Infectious Diseases Staphylococcus aureus Ovariectomy Pro-inflammatory cytokines Ovarian hormones |
| title | Ovarian hormones influence immune response to Staphylococcus aureus infection |
| title_full | Ovarian hormones influence immune response to Staphylococcus aureus infection |
| title_fullStr | Ovarian hormones influence immune response to Staphylococcus aureus infection |
| title_full_unstemmed | Ovarian hormones influence immune response to Staphylococcus aureus infection |
| title_short | Ovarian hormones influence immune response to Staphylococcus aureus infection |
| title_sort | ovarian hormones influence immune response to staphylococcus aureus infection |
| topic | Staphylococcus aureus Ovariectomy Pro-inflammatory cytokines Ovarian hormones |
| url | http://www.sciencedirect.com/science/article/pii/S1413867020301562 |
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