Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymers
Cationic copolymers based on 2-(N,<i>N</i>-dimethylamino)ethyl methacrylate and polyethylene glycol monomethyl ether (pDMAEMA-co-PEO) with different molecular weights have been synthesized. Their physicochemical properties were studied by NMR spectroscopy, sedimentation, and potentiometr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/14/3036 |
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| author | Tatiana P. Loginova Irina A. Khotina Yurii A. Kabachii Sergei Yu. Kochev Vyacheslav M. Abramov Valentin S. Khlebnikov Natalia L. Kulikova Yaroslav O. Mezhuev |
| author_facet | Tatiana P. Loginova Irina A. Khotina Yurii A. Kabachii Sergei Yu. Kochev Vyacheslav M. Abramov Valentin S. Khlebnikov Natalia L. Kulikova Yaroslav O. Mezhuev |
| author_sort | Tatiana P. Loginova |
| collection | DOAJ |
| description | Cationic copolymers based on 2-(N,<i>N</i>-dimethylamino)ethyl methacrylate and polyethylene glycol monomethyl ether (pDMAEMA-co-PEO) with different molecular weights have been synthesized. Their physicochemical properties were studied by NMR spectroscopy, sedimentation, and potentiometric titration. According to the data of potentiometric titration for the synthesized pegylated cationic copolymers, the apparent dissociation constants were determined in the pH range from 4.5 to 8.5. The physicochemical properties of interpolyelectrolyte complexes of these polycations with circular DNA (IPEC DNA) were also studied by dynamic light scattering, electrophoretic mobility, and TEM methods. It has been established that the diameter and electrokinetic potential (ζ-potential) of interpolyelectrolyte complexes can be varied over a wide range (from 200 nm to 1.5 μm and from −25 mV to +30 mV) by changing the ratio of oppositely charged ionizable groups in pegylated cationic copolymers and DNA, as well as by regulating medium pH. The resistance of the IPEC DNA/polycation complex to the action of nucleases was studied by electrophoresis in agarose gel; the cytotoxic effect of the polymers in vitro, and the efficiency of penetration (transfection) of IPEC DNA with PDMAEMA-co-PEO-polycations into eukaryotic cells of a cell line derived from human embryonic kidneys HEK 293 in vitro. |
| first_indexed | 2024-03-11T00:42:50Z |
| format | Article |
| id | doaj.art-43201de31120480ebdf070bd4914ef35 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T00:42:50Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-43201de31120480ebdf070bd4914ef352023-11-18T21:02:25ZengMDPI AGPolymers2073-43602023-07-011514303610.3390/polym15143036Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate CopolymersTatiana P. Loginova0Irina A. Khotina1Yurii A. Kabachii2Sergei Yu. Kochev3Vyacheslav M. Abramov4Valentin S. Khlebnikov5Natalia L. Kulikova6Yaroslav O. Mezhuev7A.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, RussiaA.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, RussiaA.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, RussiaA.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, RussiaJSC Institute Immunological Engineering, Nauchnaya street 1, 142380 Lybuchany, Moscow District, Moscow Region, RussiaJSC Institute Immunological Engineering, Nauchnaya street 1, 142380 Lybuchany, Moscow District, Moscow Region, RussiaJSC Institute Immunological Engineering, Nauchnaya street 1, 142380 Lybuchany, Moscow District, Moscow Region, RussiaA.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, RussiaCationic copolymers based on 2-(N,<i>N</i>-dimethylamino)ethyl methacrylate and polyethylene glycol monomethyl ether (pDMAEMA-co-PEO) with different molecular weights have been synthesized. Their physicochemical properties were studied by NMR spectroscopy, sedimentation, and potentiometric titration. According to the data of potentiometric titration for the synthesized pegylated cationic copolymers, the apparent dissociation constants were determined in the pH range from 4.5 to 8.5. The physicochemical properties of interpolyelectrolyte complexes of these polycations with circular DNA (IPEC DNA) were also studied by dynamic light scattering, electrophoretic mobility, and TEM methods. It has been established that the diameter and electrokinetic potential (ζ-potential) of interpolyelectrolyte complexes can be varied over a wide range (from 200 nm to 1.5 μm and from −25 mV to +30 mV) by changing the ratio of oppositely charged ionizable groups in pegylated cationic copolymers and DNA, as well as by regulating medium pH. The resistance of the IPEC DNA/polycation complex to the action of nucleases was studied by electrophoresis in agarose gel; the cytotoxic effect of the polymers in vitro, and the efficiency of penetration (transfection) of IPEC DNA with PDMAEMA-co-PEO-polycations into eukaryotic cells of a cell line derived from human embryonic kidneys HEK 293 in vitro.https://www.mdpi.com/2073-4360/15/14/3036polycationsDNAtransfectionHEK 293interpolymer complexDNA carriers |
| spellingShingle | Tatiana P. Loginova Irina A. Khotina Yurii A. Kabachii Sergei Yu. Kochev Vyacheslav M. Abramov Valentin S. Khlebnikov Natalia L. Kulikova Yaroslav O. Mezhuev Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymers Polymers polycations DNA transfection HEK 293 interpolymer complex DNA carriers |
| title | Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymers |
| title_full | Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymers |
| title_fullStr | Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymers |
| title_full_unstemmed | Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymers |
| title_short | Promising Gene Delivery Properties of Polycations Based on 2-(N,<i>N</i>-dimethylamino)ethyl Methacrylate and Polyethylene Glycol Monomethyl Ether Methacrylate Copolymers |
| title_sort | promising gene delivery properties of polycations based on 2 n i n i dimethylamino ethyl methacrylate and polyethylene glycol monomethyl ether methacrylate copolymers |
| topic | polycations DNA transfection HEK 293 interpolymer complex DNA carriers |
| url | https://www.mdpi.com/2073-4360/15/14/3036 |
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