Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022
Crimean–Congo hemorrhagic fever (CCHF), the most widespread tick-borne viral human infection, poses a threat to global health. In this study, clinical samples collected through national surveillance systems were screened for acute CCHF virus (CCHFV) infection using RT-PCR and for exposure using ELIS...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/2/315 |
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| author | Ousseynou Sene Samba Niang Sagne Déthié Ngom Moussa Moise Diagne Aminata Badji Aliou Khoulé El Hadji Ndiaye Safietou Sankhe Cheikh Loucoubar Mawlouth Diallo Manfred Weidmann Ndongo Dia Etienne Simon-Lorière Yoro Sall Boly Diop Mamadou Ndiaye Anavaj Sakuntabhai Amadou Alpha Sall Ousmane Faye Oumar Faye Diawo Diallo Mamadou Aliou Barry Gamou Fall |
| author_facet | Ousseynou Sene Samba Niang Sagne Déthié Ngom Moussa Moise Diagne Aminata Badji Aliou Khoulé El Hadji Ndiaye Safietou Sankhe Cheikh Loucoubar Mawlouth Diallo Manfred Weidmann Ndongo Dia Etienne Simon-Lorière Yoro Sall Boly Diop Mamadou Ndiaye Anavaj Sakuntabhai Amadou Alpha Sall Ousmane Faye Oumar Faye Diawo Diallo Mamadou Aliou Barry Gamou Fall |
| author_sort | Ousseynou Sene |
| collection | DOAJ |
| description | Crimean–Congo hemorrhagic fever (CCHF), the most widespread tick-borne viral human infection, poses a threat to global health. In this study, clinical samples collected through national surveillance systems were screened for acute CCHF virus (CCHFV) infection using RT-PCR and for exposure using ELISA. For any CCHF-positive sample, livestock and tick samples were also collected in the neighborhood of the confirmed case and tested using ELISA and RT-PCR, respectively. Genome sequencing and phylogenetic analyses were also performed on samples with positive RT-PCR results. In Eastern Senegal, two human cases and one <i>Hyalomma</i> tick positive for CCHF were identified and a seroprevalence in livestock ranging from 9.33% to 45.26% was detected. Phylogenetic analyses revealed that the human strain belonged to genotype I based on the available L segment. However, the tick strain showed a reassortant profile, with the L and M segments belonging to genotype I and the S segment belonging to genotype III. Our data also showed that our strains clustered with strains isolated in different countries, including Mauritania. Therefore, our findings confirmed the high genetic variability inside the CCHF genotypes and their introduction to Senegal from other countries. They also indicate an increasing CCHF threat in Senegal and emphasize the need to reinforce surveillance using a one-health approach. |
| first_indexed | 2024-03-07T22:10:00Z |
| format | Article |
| id | doaj.art-4327e2867d694715a128f2a9d960d7c3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-07T22:10:00Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4327e2867d694715a128f2a9d960d7c32024-02-23T15:37:47ZengMDPI AGViruses1999-49152024-02-0116231510.3390/v16020315Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022Ousseynou Sene0Samba Niang Sagne1Déthié Ngom2Moussa Moise Diagne3Aminata Badji4Aliou Khoulé5El Hadji Ndiaye6Safietou Sankhe7Cheikh Loucoubar8Mawlouth Diallo9Manfred Weidmann10Ndongo Dia11Etienne Simon-Lorière12Yoro Sall13Boly Diop14Mamadou Ndiaye15Anavaj Sakuntabhai16Amadou Alpha Sall17Ousmane Faye18Oumar Faye19Diawo Diallo20Mamadou Aliou Barry21Gamou Fall22WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalEpidemiology, Clinical Research & Data Science, Pasteur Institute, Dakar 12900, SenegalZoology Department, Pasteur Institute, Dakar 12900, SenegalWHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalZoology Department, Pasteur Institute, Dakar 12900, SenegalZoology Department, Pasteur Institute, Dakar 12900, SenegalZoology Department, Pasteur Institute, Dakar 12900, SenegalWHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalEpidemiology, Clinical Research & Data Science, Pasteur Institute, Dakar 12900, SenegalZoology Department, Pasteur Institute, Dakar 12900, SenegalInstitute of Microbiology and Virology, Brandenburg Medical School, 01968 Brandenburg, GermanyWHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalG5 Evolutionary Genomics of RNA Viruses, Pasteur Institute, 75015 Paris, FranceMinistry of Health, Dakar 10700, SenegalMinistry of Health, Dakar 10700, SenegalMinistry of Health, Dakar 10700, SenegalFunctional Genetics of Infectious Disease Unit, Pasteur Institute, 75015 Paris, FranceWHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalWHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalWHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalZoology Department, Pasteur Institute, Dakar 12900, SenegalEpidemiology, Clinical Research & Data Science, Pasteur Institute, Dakar 12900, SenegalWHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fevers, Virology Department, Pasteur Institute, Dakar 12900, SenegalCrimean–Congo hemorrhagic fever (CCHF), the most widespread tick-borne viral human infection, poses a threat to global health. In this study, clinical samples collected through national surveillance systems were screened for acute CCHF virus (CCHFV) infection using RT-PCR and for exposure using ELISA. For any CCHF-positive sample, livestock and tick samples were also collected in the neighborhood of the confirmed case and tested using ELISA and RT-PCR, respectively. Genome sequencing and phylogenetic analyses were also performed on samples with positive RT-PCR results. In Eastern Senegal, two human cases and one <i>Hyalomma</i> tick positive for CCHF were identified and a seroprevalence in livestock ranging from 9.33% to 45.26% was detected. Phylogenetic analyses revealed that the human strain belonged to genotype I based on the available L segment. However, the tick strain showed a reassortant profile, with the L and M segments belonging to genotype I and the S segment belonging to genotype III. Our data also showed that our strains clustered with strains isolated in different countries, including Mauritania. Therefore, our findings confirmed the high genetic variability inside the CCHF genotypes and their introduction to Senegal from other countries. They also indicate an increasing CCHF threat in Senegal and emphasize the need to reinforce surveillance using a one-health approach.https://www.mdpi.com/1999-4915/16/2/315Crimean–Congo hemorrhagic fever virushumanticksreassortmentEastern Senegal |
| spellingShingle | Ousseynou Sene Samba Niang Sagne Déthié Ngom Moussa Moise Diagne Aminata Badji Aliou Khoulé El Hadji Ndiaye Safietou Sankhe Cheikh Loucoubar Mawlouth Diallo Manfred Weidmann Ndongo Dia Etienne Simon-Lorière Yoro Sall Boly Diop Mamadou Ndiaye Anavaj Sakuntabhai Amadou Alpha Sall Ousmane Faye Oumar Faye Diawo Diallo Mamadou Aliou Barry Gamou Fall Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022 Viruses Crimean–Congo hemorrhagic fever virus human ticks reassortment Eastern Senegal |
| title | Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022 |
| title_full | Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022 |
| title_fullStr | Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022 |
| title_full_unstemmed | Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022 |
| title_short | Emergence of Crimean–Congo Hemorrhagic Fever Virus in Eastern Senegal in 2022 |
| title_sort | emergence of crimean congo hemorrhagic fever virus in eastern senegal in 2022 |
| topic | Crimean–Congo hemorrhagic fever virus human ticks reassortment Eastern Senegal |
| url | https://www.mdpi.com/1999-4915/16/2/315 |
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