Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease
Context: Safranal (SAF) is verified to have potential effects in promoting nerve growth. Objectives: This study verifies the role of SAF in promoting dopaminergic neurons growth in vitro and in vivo. Material and methods: Rat neural stem cells (NSC) were treated with 1, 20, or 100 ng/mL of SAF, and...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2018.1501705 |
| _version_ | 1818790377446965248 |
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| author | Yi Zhao Gangming Xi |
| author_facet | Yi Zhao Gangming Xi |
| author_sort | Yi Zhao |
| collection | DOAJ |
| description | Context: Safranal (SAF) is verified to have potential effects in promoting nerve growth. Objectives: This study verifies the role of SAF in promoting dopaminergic neurons growth in vitro and in vivo. Material and methods: Rat neural stem cells (NSC) were treated with 1, 20, or 100 ng/mL of SAF, and the expression levels of tyrosine hydroxylase (TH) and dopamine transporter (DAT) were assayed by flow cytometry and real-time PCR and the secretion of dopamine (DA) was assayed by ELISA. Then, 2 × 106 cells of SAF-treated NSC was administrated into PD rat models induced by 6-OHDA. The differentiation and survival of dopaminergic neurons was identified by fluorescence microscope and TH+ cells by immunostaining and DA secretion by ELISA at week 2 and week 4, respectively. Results: After being treated with SAF at 20 and 100 ng/mL for 1 week, TH and DAT positive rates increased 1.4- and 1.7-fold (p < 0.01, respectively). TH and DAT mRNA also increased 8.05- and 4.41-fold, respectively. And the release of DA statistically increased 1.5-fold (p < 0.01). In vivo, the number of rotations decreased to 4.33 ± 0.97 rpm (p < 0.01) and the survival rates increased to 77.66 ± 7.87% (p < 0.05) at week 4 after transplantation of SAF-treated NSC. Moreover, the transplanted cells increased three-fold, TH fluorescence density increased four-fold and DA releases increased 1.4-fold (p < 0.01) at week 4 after transplantation. Conclusions: SAF promoted the production of functional DA cells and alleviated PD, which may contribute to a new therapy for PD patients. |
| first_indexed | 2024-12-18T14:54:29Z |
| format | Article |
| id | doaj.art-432a1f44cf9041c8b93ce877345329b4 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-18T14:54:29Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-432a1f44cf9041c8b93ce877345329b42022-12-21T21:04:05ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162018-01-0156145045410.1080/13880209.2018.15017051501705Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s diseaseYi Zhao0Gangming Xi1Neurology Department of Xuhui Central HospitalNeurology Department of Xuhui Central HospitalContext: Safranal (SAF) is verified to have potential effects in promoting nerve growth. Objectives: This study verifies the role of SAF in promoting dopaminergic neurons growth in vitro and in vivo. Material and methods: Rat neural stem cells (NSC) were treated with 1, 20, or 100 ng/mL of SAF, and the expression levels of tyrosine hydroxylase (TH) and dopamine transporter (DAT) were assayed by flow cytometry and real-time PCR and the secretion of dopamine (DA) was assayed by ELISA. Then, 2 × 106 cells of SAF-treated NSC was administrated into PD rat models induced by 6-OHDA. The differentiation and survival of dopaminergic neurons was identified by fluorescence microscope and TH+ cells by immunostaining and DA secretion by ELISA at week 2 and week 4, respectively. Results: After being treated with SAF at 20 and 100 ng/mL for 1 week, TH and DAT positive rates increased 1.4- and 1.7-fold (p < 0.01, respectively). TH and DAT mRNA also increased 8.05- and 4.41-fold, respectively. And the release of DA statistically increased 1.5-fold (p < 0.01). In vivo, the number of rotations decreased to 4.33 ± 0.97 rpm (p < 0.01) and the survival rates increased to 77.66 ± 7.87% (p < 0.05) at week 4 after transplantation of SAF-treated NSC. Moreover, the transplanted cells increased three-fold, TH fluorescence density increased four-fold and DA releases increased 1.4-fold (p < 0.01) at week 4 after transplantation. Conclusions: SAF promoted the production of functional DA cells and alleviated PD, which may contribute to a new therapy for PD patients.http://dx.doi.org/10.1080/13880209.2018.1501705neural stem celltyrosine hydroxylase |
| spellingShingle | Yi Zhao Gangming Xi Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease Pharmaceutical Biology neural stem cell tyrosine hydroxylase |
| title | Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease |
| title_full | Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease |
| title_fullStr | Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease |
| title_full_unstemmed | Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease |
| title_short | Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease |
| title_sort | safranal promoted differentiation and survival of dopaminergic neurons in an animal model of parkinson s disease |
| topic | neural stem cell tyrosine hydroxylase |
| url | http://dx.doi.org/10.1080/13880209.2018.1501705 |
| work_keys_str_mv | AT yizhao safranalpromoteddifferentiationandsurvivalofdopaminergicneuronsinananimalmodelofparkinsonsdisease AT gangmingxi safranalpromoteddifferentiationandsurvivalofdopaminergicneuronsinananimalmodelofparkinsonsdisease |