A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo
In this study, the aerial parts of <i>Moricandia sinaica</i> were evaluated for their in vivo analgesic, anti-inflammatory and antipyretic activities. The analgesic activities were examined using acetic acid-induced writhing, the hot plate test and the tail flick method. The anti-inflamm...
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/21/5049 |
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| author | Sahar El-mekkawy Abdelaaty A. Shahat Ali S. Alqahtani Mansour S. Alsaid Mohamed A.O. Abdelfattah Riaz Ullah Mahmoud Emam Abdelaziz Yasri Mansour Sobeh |
| author_facet | Sahar El-mekkawy Abdelaaty A. Shahat Ali S. Alqahtani Mansour S. Alsaid Mohamed A.O. Abdelfattah Riaz Ullah Mahmoud Emam Abdelaziz Yasri Mansour Sobeh |
| author_sort | Sahar El-mekkawy |
| collection | DOAJ |
| description | In this study, the aerial parts of <i>Moricandia sinaica</i> were evaluated for their in vivo analgesic, anti-inflammatory and antipyretic activities. The analgesic activities were examined using acetic acid-induced writhing, the hot plate test and the tail flick method. The anti-inflammatory and the antipyretic activities were evaluated using carrageenan-induced paw edema in rats and brewer’s yeast-induced pyrexia in mice, respectively. The aqueous fraction of the methanol extract (MS-3) showed to be the most bioactive among the other investigated fractions. At the dose of 500 mg/kg, the fraction (MS-3) showed a significant percentage inhibition of the carrageenan-induced edema by 52.4% (<i>p</i> < 0.05). In addition, MS-3 exhibited a significant inhibition of acetic acid-induced writhes by 44.4% and 61.5% (<i>p</i> < 0.001) at 250-mg/kg and 500-mg/kg doses, respectively. At 120 min post-treatment, the rat groups treated with MS-3 displayed statistically significant reduction in rectal temperature (<i>p</i> < 0.001) by 1.7 °C and 2.2 °C at 250- and 500-mg/kg doses, respectively. The phytochemical composition of the fraction (MS-3) was characterized by high-performance liquid chromatography-mass spectrometry (HPLC-PDA-MS/MS). Molecular docking studies demonstrated that the polyphenols identified in MS-3 revealed good binding energy upon docking to some target proteins involved in pain response and inflammation, such as the cannabinoid receptors CB1 and CB2, the fatty acid amide hydrolase (FAAH) and the cyclooxygenase COX-1 and COX-2 enzymes. Based on the findings from the present work, it could be concluded that the aerial parts extract of <i>M. sinaica</i> exerts potential analgesic, anti-inflammatory and antipyretic effects in rats. |
| first_indexed | 2024-03-10T15:11:48Z |
| format | Article |
| id | doaj.art-432aed12a80a47eb8003d96d03f93b0d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:11:48Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-432aed12a80a47eb8003d96d03f93b0d2023-11-20T19:14:32ZengMDPI AGMolecules1420-30492020-10-012521504910.3390/molecules25215049A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In VivoSahar El-mekkawy0Abdelaaty A. Shahat1Ali S. Alqahtani2Mansour S. Alsaid3Mohamed A.O. Abdelfattah4Riaz Ullah5Mahmoud Emam6Abdelaziz Yasri7Mansour Sobeh8Department of Chemistry of Natural Compounds, National Research Centre, Dokki, Giza 12622, EgyptPharmacognosy Department, Medicinal Aromatic and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh 11451, Saudi ArabiaPharmacognosy Department, Medicinal Aromatic and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh 11451, Saudi ArabiaPharmacognosy Department, Medicinal Aromatic and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh 11451, Saudi ArabiaCollege of Engineering and Technology, American University of the Middle East, KuwaitPharmacognosy Department, Medicinal Aromatic and Poisonous Plants Research Center, College of Pharmacy King Saud University, Riyadh 11451, Saudi ArabiaCollege of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, ChinaAgroBioSciences Research Division, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, 43150 Ben-Guerir, MoroccoAgroBioSciences Research Division, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, 43150 Ben-Guerir, MoroccoIn this study, the aerial parts of <i>Moricandia sinaica</i> were evaluated for their in vivo analgesic, anti-inflammatory and antipyretic activities. The analgesic activities were examined using acetic acid-induced writhing, the hot plate test and the tail flick method. The anti-inflammatory and the antipyretic activities were evaluated using carrageenan-induced paw edema in rats and brewer’s yeast-induced pyrexia in mice, respectively. The aqueous fraction of the methanol extract (MS-3) showed to be the most bioactive among the other investigated fractions. At the dose of 500 mg/kg, the fraction (MS-3) showed a significant percentage inhibition of the carrageenan-induced edema by 52.4% (<i>p</i> < 0.05). In addition, MS-3 exhibited a significant inhibition of acetic acid-induced writhes by 44.4% and 61.5% (<i>p</i> < 0.001) at 250-mg/kg and 500-mg/kg doses, respectively. At 120 min post-treatment, the rat groups treated with MS-3 displayed statistically significant reduction in rectal temperature (<i>p</i> < 0.001) by 1.7 °C and 2.2 °C at 250- and 500-mg/kg doses, respectively. The phytochemical composition of the fraction (MS-3) was characterized by high-performance liquid chromatography-mass spectrometry (HPLC-PDA-MS/MS). Molecular docking studies demonstrated that the polyphenols identified in MS-3 revealed good binding energy upon docking to some target proteins involved in pain response and inflammation, such as the cannabinoid receptors CB1 and CB2, the fatty acid amide hydrolase (FAAH) and the cyclooxygenase COX-1 and COX-2 enzymes. Based on the findings from the present work, it could be concluded that the aerial parts extract of <i>M. sinaica</i> exerts potential analgesic, anti-inflammatory and antipyretic effects in rats.https://www.mdpi.com/1420-3049/25/21/5049<i>Moricandia sinaica</i>analgesicanti-inflammatoryantipyreticLC-MS/MS |
| spellingShingle | Sahar El-mekkawy Abdelaaty A. Shahat Ali S. Alqahtani Mansour S. Alsaid Mohamed A.O. Abdelfattah Riaz Ullah Mahmoud Emam Abdelaziz Yasri Mansour Sobeh A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo Molecules <i>Moricandia sinaica</i> analgesic anti-inflammatory antipyretic LC-MS/MS |
| title | A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo |
| title_full | A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo |
| title_fullStr | A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo |
| title_full_unstemmed | A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo |
| title_short | A Polyphenols-Rich Extract from <i>Moricandia sinaica</i> Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo |
| title_sort | polyphenols rich extract from i moricandia sinaica i boiss exhibits analgesic anti inflammatory and antipyretic activities in vivo |
| topic | <i>Moricandia sinaica</i> analgesic anti-inflammatory antipyretic LC-MS/MS |
| url | https://www.mdpi.com/1420-3049/25/21/5049 |
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