Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel ()
The objectives of this study were to determine the molecular weight of condensed tannins (CT) extracted from mangosteen (Garcinia mangostana L) peel, its protein binding affinity and effects on fermentation parameters including total gas, methane (CH4) and volatile fatty acids (VFA) production. The...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2015-10-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-28-10-1442.pdf |
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| author | P. Paengkoum T. Phonmun J. B. Liang X. D. Huang H. Y. Tan M. F. Jahromi |
| author_facet | P. Paengkoum T. Phonmun J. B. Liang X. D. Huang H. Y. Tan M. F. Jahromi |
| author_sort | P. Paengkoum |
| collection | DOAJ |
| description | The objectives of this study were to determine the molecular weight of condensed tannins (CT) extracted from mangosteen (Garcinia mangostana L) peel, its protein binding affinity and effects on fermentation parameters including total gas, methane (CH4) and volatile fatty acids (VFA) production. The average molecular weight (Mw) of the purified CT was 2,081 Da with a protein binding affinity of 0.69 (the amount needed to bind half the maximum bovine serum albumin). In vitro gas production declined by 0.409, 0.121, and 0.311, respectively, while CH4 production decreased by 0.211, 0.353, and 0.549, respectively, with addition of 10, 20, and 30 mg CT/500 mg dry matter (DM) compared to the control (p<0.05). The effects of CT from mangosteen-peel on in vitro DM degradability (IVDMD) and in vitro N degradability was negative and linear (p<0.01). Total VFA, concentrations of acetic, propionic, butyric and isovaleric acids decreased linearly with increasing amount of CT. The aforementioned results show that protein binding affinity of CT from mangosteen-peel is lower than those reported for Leucaena forages, however, the former has stronger negative effect on IVDMD. Therefore, the use of mangosteen-peel as protein source and CH4 mitigating agent in ruminant feed requires further investigations. |
| first_indexed | 2024-12-24T13:07:33Z |
| format | Article |
| id | doaj.art-433303de51ea42ef956acc77addc894f |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-24T13:07:33Z |
| publishDate | 2015-10-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-433303de51ea42ef956acc77addc894f2022-12-21T16:53:57ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172015-10-0128101442144810.5713/ajas.13.083423252Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel ()P. PaengkoumT. Phonmun0J. B. Liang1X. D. Huang2H. Y. Tan3M. F. Jahromi4 King Mongkut Institute of Technology Ladkrabang Chumphon Campus, Pathio, Chumphon 86160, .Thailand Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, .Malaysia Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, .Malaysia School of Public Health, Lanzhou University, Lanzhou 730000, .China Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, .MalaysiaThe objectives of this study were to determine the molecular weight of condensed tannins (CT) extracted from mangosteen (Garcinia mangostana L) peel, its protein binding affinity and effects on fermentation parameters including total gas, methane (CH4) and volatile fatty acids (VFA) production. The average molecular weight (Mw) of the purified CT was 2,081 Da with a protein binding affinity of 0.69 (the amount needed to bind half the maximum bovine serum albumin). In vitro gas production declined by 0.409, 0.121, and 0.311, respectively, while CH4 production decreased by 0.211, 0.353, and 0.549, respectively, with addition of 10, 20, and 30 mg CT/500 mg dry matter (DM) compared to the control (p<0.05). The effects of CT from mangosteen-peel on in vitro DM degradability (IVDMD) and in vitro N degradability was negative and linear (p<0.01). Total VFA, concentrations of acetic, propionic, butyric and isovaleric acids decreased linearly with increasing amount of CT. The aforementioned results show that protein binding affinity of CT from mangosteen-peel is lower than those reported for Leucaena forages, however, the former has stronger negative effect on IVDMD. Therefore, the use of mangosteen-peel as protein source and CH4 mitigating agent in ruminant feed requires further investigations.http://www.ajas.info/upload/pdf/ajas-28-10-1442.pdfCondensed TanninsMangosteen-peelMethane ProductionMolecular WeightProtein Binding Affinity |
| spellingShingle | P. Paengkoum T. Phonmun J. B. Liang X. D. Huang H. Y. Tan M. F. Jahromi Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel () Asian-Australasian Journal of Animal Sciences Condensed Tannins Mangosteen-peel Methane Production Molecular Weight Protein Binding Affinity |
| title | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel () |
| title_full | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel () |
| title_fullStr | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel () |
| title_full_unstemmed | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel () |
| title_short | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel () |
| title_sort | molecular weight protein binding affinity and methane mitigation of condensed tannins from mangosteen peel |
| topic | Condensed Tannins Mangosteen-peel Methane Production Molecular Weight Protein Binding Affinity |
| url | http://www.ajas.info/upload/pdf/ajas-28-10-1442.pdf |
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