Evaluation of nigella sativa's cold press oil as vaccine adjuvant
<b>Aim: </b>In order to identify an organic and plant based, less toxic, cheaper and more effective adjuvant, a cold pressed oil of Nigella sativa (CPNSO) and one of the essential components of Nigella sativa oil (NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant candidate...
| Главные авторы: | , , , , |
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| Формат: | Статья |
| Язык: | English |
| Опубликовано: |
Selcuk University Press
2020-10-01
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| Серии: | Eurasian Journal of Veterinary Sciences |
| Предметы: | |
| Online-ссылка: | http://eurasianjvetsci.org/pdf.php3?id=1298 |
| Итог: | <b>Aim: </b>In order to identify an organic and plant based, less toxic, cheaper
and more effective adjuvant, a cold pressed oil of Nigella sativa
(CPNSO) and one of the essential components of Nigella sativa oil
(NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant
candidates.<p>
<b>Materials and Methods:</b> Adjuvant potentials of the both were measured
by examining specific antiibody titers to ovalbumin given in
presence of either of CPNSO or thymoquinone, in vivo. Such potentials
of thymoquinone alone (A1), CPNSO (A2), mCPNSO (experimentally
formed by addition of thymoquinone to CPNSO making up
mCPNSO that was contained 10% more thymoquinone than original
CPNSO did; A3) and Al(OH)3 (A4) in presence of ovalbumin in Swiss
albino mice (n=36, female). The effects were determined by measuring
anti-ovalbumin antibodies after immunization to ovalbumin by
home-made ELISA in mice.<p>
<b>Results:</b> Sera and conjugate were optimally diluted 1/200 and
1/40.000, respectively. Mean OD values at 450 nm of the groups
control (A0), A1, A2, A3 and A4 were 0,043 (± 0,002), 0,668 (±
0,074), 0,644 (± 0,018), 0,675 (± 0,066), and 0,745 (± 0,09), respectively.<p>
<b>Conclusion:</b> By comparison control, all three (A1, A2, A3) of the test
formulations were found to be as effective as commercial (A4) formulation
in triggering humoral response to ovalbumin (p > 0,05).
Therefore, each of Nigella sativa based adjuvant candidates has an
alternative potential to Al(OH)3 in the mouse. |
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| ISSN: | 1309-6958 2146-1953 |