A case report of sporadic ovine listerial meningoencephalitis in Kosovo
In 2018, a case of neural disease suspected of listeriosis was reported in a flock of sheep in Kosovo with the death of ewes and 5 lambs. Samples from the brain of only three dead animals were subjected to histopathological and bacteriological analysis. MALDI-TOF MS was applied to confirm suspected...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2020-07-01
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| Series: | Veterinaria Italiana |
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| Online Access: | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2166 |
| _version_ | 1818774213797871616 |
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| author | Afrim Hamidi Bledar Bisha Izedin Goga Baolin Wang Avni Robaj Driton Shefqet Sylejmani |
| author_facet | Afrim Hamidi Bledar Bisha Izedin Goga Baolin Wang Avni Robaj Driton Shefqet Sylejmani |
| author_sort | Afrim Hamidi |
| collection | DOAJ |
| description | In 2018, a case of neural disease suspected of listeriosis was reported in a flock of sheep in Kosovo with the death of ewes and 5 lambs. Samples from the brain of only three dead animals were subjected to histopathological and bacteriological analysis. MALDI-TOF MS was applied to confirm suspected Listeria spp. isolates from culture and multiplex PCR was applied for molecular serotyping. All isolates were tested for antimicrobial susceptibility by microdilution broth method. The histopathological analysis of the brain specimens showed typical changes for Listeria monocytogenes. Listeria spp. was isolated in brain samples from all three animals, and all the isolates were confirmed using MALDI-TOF MS and PCR down to the species level (Listeria monocytogenes). The molecular characterisation using multiplex PCR revealed all isolates as Listeria monocytogenes serotype 4b. All L. monocytogenes isolates were found to be susceptible to penicillin, erythromycin, tetracycline, streptomycin, trimethoprim/sulfamethosazole, quinupristin/dalfopristin, kanamycin, vancomycin, and gentamicin but resistant to nitrofurantoin and lincomycin. This study shows the emergence of a highly virulent strain in sheep farms in Kosovo and a possible threat to public health. |
| first_indexed | 2024-12-18T10:37:35Z |
| format | Article |
| id | doaj.art-4339e95d8bdb46df82cc4fe63d86b4d7 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-12-18T10:37:35Z |
| publishDate | 2020-07-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-4339e95d8bdb46df82cc4fe63d86b4d72022-12-21T21:10:42ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272020-07-0156310.12834/2166.12781.3A case report of sporadic ovine listerial meningoencephalitis in KosovoAfrim Hamidi0Bledar Bisha1Izedin Goga2Baolin Wang3Avni Robaj4Driton Shefqet Sylejmani5Department of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina “Hasan Prishtina”, Republic of KosovoDepartment of Animal Science, University of Wyoming, Laramie, WY 82071, United States of AmericaDepartment of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina “Hasan Prishtina”, Republic of KosovoDepartment of Animal Science, University of Wyoming, Laramie, WY 82071, United States of AmericaDepartment of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina “Hasan Prishtina”, Republic of KosovoDepartment of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina “Hasan Prishtina”, Republic of KosovoIn 2018, a case of neural disease suspected of listeriosis was reported in a flock of sheep in Kosovo with the death of ewes and 5 lambs. Samples from the brain of only three dead animals were subjected to histopathological and bacteriological analysis. MALDI-TOF MS was applied to confirm suspected Listeria spp. isolates from culture and multiplex PCR was applied for molecular serotyping. All isolates were tested for antimicrobial susceptibility by microdilution broth method. The histopathological analysis of the brain specimens showed typical changes for Listeria monocytogenes. Listeria spp. was isolated in brain samples from all three animals, and all the isolates were confirmed using MALDI-TOF MS and PCR down to the species level (Listeria monocytogenes). The molecular characterisation using multiplex PCR revealed all isolates as Listeria monocytogenes serotype 4b. All L. monocytogenes isolates were found to be susceptible to penicillin, erythromycin, tetracycline, streptomycin, trimethoprim/sulfamethosazole, quinupristin/dalfopristin, kanamycin, vancomycin, and gentamicin but resistant to nitrofurantoin and lincomycin. This study shows the emergence of a highly virulent strain in sheep farms in Kosovo and a possible threat to public health.https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2166Antimicrobial susceptibilityBrainMICOvine listeriosisSerotyping |
| spellingShingle | Afrim Hamidi Bledar Bisha Izedin Goga Baolin Wang Avni Robaj Driton Shefqet Sylejmani A case report of sporadic ovine listerial meningoencephalitis in Kosovo Veterinaria Italiana Antimicrobial susceptibility Brain MIC Ovine listeriosis Serotyping |
| title | A case report of sporadic ovine listerial meningoencephalitis in Kosovo |
| title_full | A case report of sporadic ovine listerial meningoencephalitis in Kosovo |
| title_fullStr | A case report of sporadic ovine listerial meningoencephalitis in Kosovo |
| title_full_unstemmed | A case report of sporadic ovine listerial meningoencephalitis in Kosovo |
| title_short | A case report of sporadic ovine listerial meningoencephalitis in Kosovo |
| title_sort | case report of sporadic ovine listerial meningoencephalitis in kosovo |
| topic | Antimicrobial susceptibility Brain MIC Ovine listeriosis Serotyping |
| url | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2166 |
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