ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorder
<p>Abstract</p> <p>Background</p> <p>In recent years, the identification of peripheral biomarkers that are associated with psychiatric diseases, such as Major Depressive Disorder (MDD), has become relevant because these biomarkers may improve the efficiency of the diffe...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-09-01
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| Series: | BMC Psychiatry |
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| Online Access: | http://www.biomedcentral.com/1471-244X/12/145 |
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| author | Milanesi Elena Minelli Alessandra Cattane Nadia Cattaneo Annamaria Mora Cristina Barbon Alessandro Mallei Alessandra Popoli Maurizio Florio Vincenzo Conca Andreas Bignotti Stefano Gennarelli Massimo |
| author_facet | Milanesi Elena Minelli Alessandra Cattane Nadia Cattaneo Annamaria Mora Cristina Barbon Alessandro Mallei Alessandra Popoli Maurizio Florio Vincenzo Conca Andreas Bignotti Stefano Gennarelli Massimo |
| author_sort | Milanesi Elena |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In recent years, the identification of peripheral biomarkers that are associated with psychiatric diseases, such as Major Depressive Disorder (MDD), has become relevant because these biomarkers may improve the efficiency of the differential diagnosis process and indicate targets for new antidepressant drugs. Two recent candidate genes, <it>ErbB3</it> and <it>Fgfr1</it>, are growth factors whose mRNA levels have been found to be altered in the leukocytes of patients that are affected by bipolar disorder in a depressive state. On this basis, the aim of the study was to determine if <it>ErbB3</it> and <it>Fgfr1</it> mRNA levels could be a biomarkers of MDD.</p> <p>Methods</p> <p>We measured by Real Time PCR ErbB3 and Fgfr1 mRNA expression levels in leukocytes of MDD patients compared with controls. Successively, to assess whether ErbB3 mRNA levels were influenced by previous antidepressant treatment we stratified our patients sample in two cohorts, comparing drug-naive versus drug-free patients. Moreover, we evaluated the levels of the transcript in MDD patients after 12 weeks of antidepressant treatment, and in prefrontal cortex of rats stressed and treated with an antidepressant drug of the same class.</p> <p>Results</p> <p>These results showed that <it>ErbB3</it> but not <it>Fgfr1</it> mRNA levels were reduced in leukocytes of MDD patients compared to healthy subjects. Furthermore, <it>ErbB3</it> levels were not affected by antidepressant treatment in either human or animal models</p> <p>Conclusions</p> <p>Our data suggest that ErbB3 might be considered as a biomarker for MDD and that its deficit may underlie the pathopsysiology of the disease and is not a consequence of treatment. Moreover the study supports the usefulness of leukocytes as a peripheral system for identifying biomarkers in psychiatric diseases.</p> |
| first_indexed | 2024-12-18T19:40:21Z |
| format | Article |
| id | doaj.art-433a259c868d4451b3f346f9b267fbfe |
| institution | Directory Open Access Journal |
| issn | 1471-244X |
| language | English |
| last_indexed | 2024-12-18T19:40:21Z |
| publishDate | 2012-09-01 |
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| series | BMC Psychiatry |
| spelling | doaj.art-433a259c868d4451b3f346f9b267fbfe2022-12-21T20:55:27ZengBMCBMC Psychiatry1471-244X2012-09-0112114510.1186/1471-244X-12-145ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorderMilanesi ElenaMinelli AlessandraCattane NadiaCattaneo AnnamariaMora CristinaBarbon AlessandroMallei AlessandraPopoli MaurizioFlorio VincenzoConca AndreasBignotti StefanoGennarelli Massimo<p>Abstract</p> <p>Background</p> <p>In recent years, the identification of peripheral biomarkers that are associated with psychiatric diseases, such as Major Depressive Disorder (MDD), has become relevant because these biomarkers may improve the efficiency of the differential diagnosis process and indicate targets for new antidepressant drugs. Two recent candidate genes, <it>ErbB3</it> and <it>Fgfr1</it>, are growth factors whose mRNA levels have been found to be altered in the leukocytes of patients that are affected by bipolar disorder in a depressive state. On this basis, the aim of the study was to determine if <it>ErbB3</it> and <it>Fgfr1</it> mRNA levels could be a biomarkers of MDD.</p> <p>Methods</p> <p>We measured by Real Time PCR ErbB3 and Fgfr1 mRNA expression levels in leukocytes of MDD patients compared with controls. Successively, to assess whether ErbB3 mRNA levels were influenced by previous antidepressant treatment we stratified our patients sample in two cohorts, comparing drug-naive versus drug-free patients. Moreover, we evaluated the levels of the transcript in MDD patients after 12 weeks of antidepressant treatment, and in prefrontal cortex of rats stressed and treated with an antidepressant drug of the same class.</p> <p>Results</p> <p>These results showed that <it>ErbB3</it> but not <it>Fgfr1</it> mRNA levels were reduced in leukocytes of MDD patients compared to healthy subjects. Furthermore, <it>ErbB3</it> levels were not affected by antidepressant treatment in either human or animal models</p> <p>Conclusions</p> <p>Our data suggest that ErbB3 might be considered as a biomarker for MDD and that its deficit may underlie the pathopsysiology of the disease and is not a consequence of treatment. Moreover the study supports the usefulness of leukocytes as a peripheral system for identifying biomarkers in psychiatric diseases.</p>http://www.biomedcentral.com/1471-244X/12/145Major Depressive Disorder MDDErbB3Fgfr1BiomarkersLeukocytes |
| spellingShingle | Milanesi Elena Minelli Alessandra Cattane Nadia Cattaneo Annamaria Mora Cristina Barbon Alessandro Mallei Alessandra Popoli Maurizio Florio Vincenzo Conca Andreas Bignotti Stefano Gennarelli Massimo ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorder BMC Psychiatry Major Depressive Disorder MDD ErbB3 Fgfr1 Biomarkers Leukocytes |
| title | ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorder |
| title_full | ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorder |
| title_fullStr | ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorder |
| title_full_unstemmed | ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorder |
| title_short | ErbB3 mRNA leukocyte levels as a biomarker for major depressive disorder |
| title_sort | erbb3 mrna leukocyte levels as a biomarker for major depressive disorder |
| topic | Major Depressive Disorder MDD ErbB3 Fgfr1 Biomarkers Leukocytes |
| url | http://www.biomedcentral.com/1471-244X/12/145 |
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