ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets
The development of countermeasures that aid in the prevention and propagation of SARS-CoV-2 infections is critical to manage the continuing crisis brought about by COVID-19. Here we present a proof-of-concept study on the use of cell-mimetic microparticles (Cytomimetics) for the interference and seq...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X21000977 |
| _version_ | 1819174840320393216 |
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| author | Thomas A. Strong Daniel Pelaez |
| author_facet | Thomas A. Strong Daniel Pelaez |
| author_sort | Thomas A. Strong |
| collection | DOAJ |
| description | The development of countermeasures that aid in the prevention and propagation of SARS-CoV-2 infections is critical to manage the continuing crisis brought about by COVID-19. Here we present a proof-of-concept study on the use of cell-mimetic microparticles (Cytomimetics) for the interference and sequestration of SARS-CoV-2 virions away from the cellular surfaces required for replication, disease manifestation, and outbreak propagation. Recombinant human ACE2 (rhACE2) functionalized onto the surface of cytomimetic particles binds the receptor binding domain (RBD) of recombinant SARS-CoV-2 spike protein with high affinity and demonstrated a stoichiometric advantage over the use of soluble rhACE2. Inhalation of rhACE2-Cytomimetic particles by mice prior to their exposure to aerosolized spike protein demonstrated the applicability of these cytomimetic particles in preventing viral protein binding to respiratory epithelial cells. Our study demonstrates the potential of an easily deliverable and highly modular technology for the control of viral infections and to complement other prophylactic countermeasures |
| first_indexed | 2024-12-22T20:45:22Z |
| format | Article |
| id | doaj.art-433aeae2589349a4bec97e40b188e62b |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-22T20:45:22Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-433aeae2589349a4bec97e40b188e62b2022-12-21T18:13:15ZengElsevierBiotechnology Reports2215-017X2021-12-0132e00681ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targetsThomas A. Strong0Daniel Pelaez1Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami, United States; Department of Cell Biology, University of Miami, United StatesBascom Palmer Eye Institute, Department of Ophthalmology, University of Miami, United States; Department of Biomedical Engineering, University of Miami College of Engineering, University of Miami, United States; Department of Cell Biology, University of Miami, United States; Corresponding author at: 1638 NW 10th Avenue, Miami, FL 33136The development of countermeasures that aid in the prevention and propagation of SARS-CoV-2 infections is critical to manage the continuing crisis brought about by COVID-19. Here we present a proof-of-concept study on the use of cell-mimetic microparticles (Cytomimetics) for the interference and sequestration of SARS-CoV-2 virions away from the cellular surfaces required for replication, disease manifestation, and outbreak propagation. Recombinant human ACE2 (rhACE2) functionalized onto the surface of cytomimetic particles binds the receptor binding domain (RBD) of recombinant SARS-CoV-2 spike protein with high affinity and demonstrated a stoichiometric advantage over the use of soluble rhACE2. Inhalation of rhACE2-Cytomimetic particles by mice prior to their exposure to aerosolized spike protein demonstrated the applicability of these cytomimetic particles in preventing viral protein binding to respiratory epithelial cells. Our study demonstrates the potential of an easily deliverable and highly modular technology for the control of viral infections and to complement other prophylactic countermeasureshttp://www.sciencedirect.com/science/article/pii/S2215017X21000977COVID-19SARS-CoV-2CytomimeticsCountermeasuresAngiotensin I converting enzyme (ACE2) carboxypeptidase |
| spellingShingle | Thomas A. Strong Daniel Pelaez ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets Biotechnology Reports COVID-19 SARS-CoV-2 Cytomimetics Countermeasures Angiotensin I converting enzyme (ACE2) carboxypeptidase |
| title | ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets |
| title_full | ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets |
| title_fullStr | ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets |
| title_full_unstemmed | ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets |
| title_short | ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets |
| title_sort | ace2 cytomimetic particles restrict sars cov 2 spike protein binding to cellular targets |
| topic | COVID-19 SARS-CoV-2 Cytomimetics Countermeasures Angiotensin I converting enzyme (ACE2) carboxypeptidase |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X21000977 |
| work_keys_str_mv | AT thomasastrong ace2cytomimeticparticlesrestrictsarscov2spikeproteinbindingtocellulartargets AT danielpelaez ace2cytomimeticparticlesrestrictsarscov2spikeproteinbindingtocellulartargets |