Activin A regulates activation of mouse neutrophils by Smad3 signalling
Activin A, a member of the transforming growth factor beta superfamily, acts as a pro-inflammatory factor in acute phase response, and influences the pathological progress of neutrophil-mediated disease. However, whether activin A can exert an effect on the activities of neutrophils remains unclear....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2017-01-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160342 |
| _version_ | 1828823391051710464 |
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| author | Yan Qi Jingyan Ge Chunhui Ma Na Wu Xueling Cui Zhonghui Liu |
| author_facet | Yan Qi Jingyan Ge Chunhui Ma Na Wu Xueling Cui Zhonghui Liu |
| author_sort | Yan Qi |
| collection | DOAJ |
| description | Activin A, a member of the transforming growth factor beta superfamily, acts as a pro-inflammatory factor in acute phase response, and influences the pathological progress of neutrophil-mediated disease. However, whether activin A can exert an effect on the activities of neutrophils remains unclear. In this study, we found that the release of activin A was enhanced from neutrophils of mouse when stimulated with lipopolysaccharide. Furthermore, neutrophils were not only the source of activin A but also the target cells in response to activin A, in which canonical activin signalling components existed, and levels of ACTRIIA, SMAD3 and p-SMAD3 proteins were elevated in activin A-treated neutrophils. Next, the role of activin A was determined in regulation of neutrophils activities. Our data revealed that activin A induced O2− release and reactive oxygen species production, promoted IL-6 release, and enhanced phagocytosis, but failed to attract neutrophils migrating across the trans-well membrane. Moreover, we found that effect of activin A on IL-6 release from the peritoneal neutrophils of mouse was significantly attenuated by in vivo Smad3 knockdown. In summary, these data demonstrate that activin A can exert an effect on neutrophils activation in an autocrine/paracrine manner through Smad3 signalling, suggesting that activin A is an important regulator of neutrophils. |
| first_indexed | 2024-12-12T13:34:12Z |
| format | Article |
| id | doaj.art-433af4f0d7e64731ab4cd18abd2cf813 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-12T13:34:12Z |
| publishDate | 2017-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-433af4f0d7e64731ab4cd18abd2cf8132022-12-22T00:22:59ZengThe Royal SocietyOpen Biology2046-24412017-01-017510.1098/rsob.160342160342Activin A regulates activation of mouse neutrophils by Smad3 signallingYan QiJingyan GeChunhui MaNa WuXueling CuiZhonghui LiuActivin A, a member of the transforming growth factor beta superfamily, acts as a pro-inflammatory factor in acute phase response, and influences the pathological progress of neutrophil-mediated disease. However, whether activin A can exert an effect on the activities of neutrophils remains unclear. In this study, we found that the release of activin A was enhanced from neutrophils of mouse when stimulated with lipopolysaccharide. Furthermore, neutrophils were not only the source of activin A but also the target cells in response to activin A, in which canonical activin signalling components existed, and levels of ACTRIIA, SMAD3 and p-SMAD3 proteins were elevated in activin A-treated neutrophils. Next, the role of activin A was determined in regulation of neutrophils activities. Our data revealed that activin A induced O2− release and reactive oxygen species production, promoted IL-6 release, and enhanced phagocytosis, but failed to attract neutrophils migrating across the trans-well membrane. Moreover, we found that effect of activin A on IL-6 release from the peritoneal neutrophils of mouse was significantly attenuated by in vivo Smad3 knockdown. In summary, these data demonstrate that activin A can exert an effect on neutrophils activation in an autocrine/paracrine manner through Smad3 signalling, suggesting that activin A is an important regulator of neutrophils.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160342neutrophilsactivin aactivin receptorinterleukin-6smad3 |
| spellingShingle | Yan Qi Jingyan Ge Chunhui Ma Na Wu Xueling Cui Zhonghui Liu Activin A regulates activation of mouse neutrophils by Smad3 signalling Open Biology neutrophils activin a activin receptor interleukin-6 smad3 |
| title | Activin A regulates activation of mouse neutrophils by Smad3 signalling |
| title_full | Activin A regulates activation of mouse neutrophils by Smad3 signalling |
| title_fullStr | Activin A regulates activation of mouse neutrophils by Smad3 signalling |
| title_full_unstemmed | Activin A regulates activation of mouse neutrophils by Smad3 signalling |
| title_short | Activin A regulates activation of mouse neutrophils by Smad3 signalling |
| title_sort | activin a regulates activation of mouse neutrophils by smad3 signalling |
| topic | neutrophils activin a activin receptor interleukin-6 smad3 |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160342 |
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