First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria
Hepatitis E is an acute self-limited or fulminant infection in humans, caused by the hepatitis E virus (HEV). This member of the Hepeviridae family has been identified in a wide range of domestic and wild animals all over the world, with a possible transmission to humans through fecal oral route, di...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1272250/full |
| _version_ | 1797681015821434880 |
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| author | Amir Agabou Mohamed Hocine Benaissa Ilyes Bouasla Luca De Sabato Sana Hireche Giovanni Ianiro Marina Monini Ilaria Di Bartolo |
| author_facet | Amir Agabou Mohamed Hocine Benaissa Ilyes Bouasla Luca De Sabato Sana Hireche Giovanni Ianiro Marina Monini Ilaria Di Bartolo |
| author_sort | Amir Agabou |
| collection | DOAJ |
| description | Hepatitis E is an acute self-limited or fulminant infection in humans, caused by the hepatitis E virus (HEV). This member of the Hepeviridae family has been identified in a wide range of domestic and wild animals all over the world, with a possible transmission to humans through fecal oral route, direct contact and ingestion of contaminated meat products, making it one of the global zoonotic and public health major concerns. Since there is no monitoring program and a lack of data on HEV in animals in Algeria, the current preliminary survey has been undertaken to elucidate the exposure to the virus in camels at abattoirs of six southern provinces of Algeria. Two-hundred and eight sera/plasma were collected and analyzed (by double antigen sandwich ELISA) for the presence of total anti-HEV antibodies, among which 35.1% were positive, but no HEV RNA could be isolated from them (by two pan-HEV nested RT-PCR and broad range real-time reverse transcription RT-PCR). The univariate analysis showed significant associations (p < 0.05) between HEV seroprevalence and province of origin, age, and sex of camels, whereas the multivariable logistic regression analysis revealed a negative impact of camels’ age on it. The obtained results confirm that HEV infection is widespread established in the camelid population of Algeria. |
| first_indexed | 2024-03-11T23:38:46Z |
| format | Article |
| id | doaj.art-433bae83cefb4e7c9f66fb3900d4f3f8 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-11T23:38:46Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-433bae83cefb4e7c9f66fb3900d4f3f82023-09-19T16:34:56ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-09-011010.3389/fvets.2023.12722501272250First serological and molecular investigation of hepatitis E virus infection in dromedary camels in AlgeriaAmir Agabou0Mohamed Hocine Benaissa1Ilyes Bouasla2Luca De Sabato3Sana Hireche4Giovanni Ianiro5Marina Monini6Ilaria Di Bartolo7PADESCA Research Laboratory, Institute of Veterinary Sciences, University Frères Mentouri Constantine 1, Constantine, AlgeriaScientific and Technical Research Centre for Arid Areas (CRSTRA), Touggourt, AlgeriaPADESCA Research Laboratory, Institute of Veterinary Sciences, University Frères Mentouri Constantine 1, Constantine, AlgeriaDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, ItalyPADESCA Research Laboratory, Institute of Veterinary Sciences, University Frères Mentouri Constantine 1, Constantine, AlgeriaDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, ItalyDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, ItalyDepartment of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, ItalyHepatitis E is an acute self-limited or fulminant infection in humans, caused by the hepatitis E virus (HEV). This member of the Hepeviridae family has been identified in a wide range of domestic and wild animals all over the world, with a possible transmission to humans through fecal oral route, direct contact and ingestion of contaminated meat products, making it one of the global zoonotic and public health major concerns. Since there is no monitoring program and a lack of data on HEV in animals in Algeria, the current preliminary survey has been undertaken to elucidate the exposure to the virus in camels at abattoirs of six southern provinces of Algeria. Two-hundred and eight sera/plasma were collected and analyzed (by double antigen sandwich ELISA) for the presence of total anti-HEV antibodies, among which 35.1% were positive, but no HEV RNA could be isolated from them (by two pan-HEV nested RT-PCR and broad range real-time reverse transcription RT-PCR). The univariate analysis showed significant associations (p < 0.05) between HEV seroprevalence and province of origin, age, and sex of camels, whereas the multivariable logistic regression analysis revealed a negative impact of camels’ age on it. The obtained results confirm that HEV infection is widespread established in the camelid population of Algeria.https://www.frontiersin.org/articles/10.3389/fvets.2023.1272250/fullhepatitis E virusELISAseroprevalencerisk factorsdromedary camelsAlgeria |
| spellingShingle | Amir Agabou Mohamed Hocine Benaissa Ilyes Bouasla Luca De Sabato Sana Hireche Giovanni Ianiro Marina Monini Ilaria Di Bartolo First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria Frontiers in Veterinary Science hepatitis E virus ELISA seroprevalence risk factors dromedary camels Algeria |
| title | First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria |
| title_full | First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria |
| title_fullStr | First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria |
| title_full_unstemmed | First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria |
| title_short | First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria |
| title_sort | first serological and molecular investigation of hepatitis e virus infection in dromedary camels in algeria |
| topic | hepatitis E virus ELISA seroprevalence risk factors dromedary camels Algeria |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1272250/full |
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