Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal Tissues
Proteomics in animal science as well as in other biological sciences is a significant tool in the post-genomic era. In proteomic studies the presence and relative abundance of expressed proteins of a cell, tissue or biological fluid is studied. Recently, the whole genome of more and more domestic an...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/1263 |
| _version_ | 1797215550195105792 |
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| author | Levente Czegledi Gabriella Gulyas Andrea Radacsi Szilvia Kusza Janka Bekefi Bela Beri Andras Javor |
| author_facet | Levente Czegledi Gabriella Gulyas Andrea Radacsi Szilvia Kusza Janka Bekefi Bela Beri Andras Javor |
| author_sort | Levente Czegledi |
| collection | DOAJ |
| description | Proteomics in animal science as well as in other biological sciences is a significant tool in the post-genomic era. In proteomic studies the presence and relative abundance of expressed proteins of a cell, tissue or biological fluid is studied. Recently, the whole genome of more and more domestic animal species is known, but genes and the transcribed mRNA have no direct effect on biological systems as they are regulated by proteins, which explain the importance of proteomics. The most common tool in proteomic approach is the two-dimensional polyacrylamide gel electrophoresis (2D PAGE), when proteins are separated by their isoelectric point followed by their mass separation as a second dimension. In this study authors used different sample preparation and protein staining methods on meat, liver and blood plasma and carried out 2D PAGE experiments. The most appropriate sample preparation methods are described in this paper. We concluded that depletion of major proteins in plasma is required but not necessary for meat and liver samples. |
| first_indexed | 2024-04-24T11:31:51Z |
| format | Article |
| id | doaj.art-4340ff781df3466f95b835b88738baf9 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:31:51Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-4340ff781df3466f95b835b88738baf92024-04-10T08:13:29ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-014312672671263Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal TissuesLevente Czegledi0Gabriella Gulyas1Andrea Radacsi2Szilvia Kusza3Janka Bekefi4Bela Beri5Andras Javor6University of Debrecen, Faculty of Agronomy, Institute of Animal Science, H-4032 Debrecen, Böszörményi Street 138, HungaryUniversity of Debrecen, Faculty of Agronomy, Institute of Animal Science, H-4032 Debrecen, Böszörményi Street 138, HungaryUniversity of Debrecen, Faculty of Agronomy, Institute of Animal Science, H-4032 Debrecen, Böszörményi Street 138, HungaryUniversity of Debrecen, Faculty of Agronomy, Institute of Animal Science, H-4032 Debrecen, Böszörményi Street 138, HungaryUniversity of Debrecen, Faculty of Agronomy, Institute of Animal Science, H-4032 Debrecen, Böszörményi Street 138, HungaryUniversity of Debrecen, Faculty of Agronomy, Institute of Animal Science, H-4032 Debrecen, Böszörményi Street 138, HungaryUniversity of Debrecen, Faculty of Agronomy, Institute of Animal Science, H-4032 Debrecen, Böszörményi Street 138, HungaryProteomics in animal science as well as in other biological sciences is a significant tool in the post-genomic era. In proteomic studies the presence and relative abundance of expressed proteins of a cell, tissue or biological fluid is studied. Recently, the whole genome of more and more domestic animal species is known, but genes and the transcribed mRNA have no direct effect on biological systems as they are regulated by proteins, which explain the importance of proteomics. The most common tool in proteomic approach is the two-dimensional polyacrylamide gel electrophoresis (2D PAGE), when proteins are separated by their isoelectric point followed by their mass separation as a second dimension. In this study authors used different sample preparation and protein staining methods on meat, liver and blood plasma and carried out 2D PAGE experiments. The most appropriate sample preparation methods are described in this paper. We concluded that depletion of major proteins in plasma is required but not necessary for meat and liver samples.https://spasb.ro/index.php/public_html/article/view/1263animal tissueprotein expressionproteome2d page |
| spellingShingle | Levente Czegledi Gabriella Gulyas Andrea Radacsi Szilvia Kusza Janka Bekefi Bela Beri Andras Javor Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal Tissues Scientific Papers Animal Science and Biotechnologies animal tissue protein expression proteome 2d page |
| title | Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal Tissues |
| title_full | Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal Tissues |
| title_fullStr | Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal Tissues |
| title_full_unstemmed | Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal Tissues |
| title_short | Sample Preparation and Staining Methods for Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins from Animal Tissues |
| title_sort | sample preparation and staining methods for two dimensional polyacrylamide gel electrophoresis of proteins from animal tissues |
| topic | animal tissue protein expression proteome 2d page |
| url | https://spasb.ro/index.php/public_html/article/view/1263 |
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