Improvement of ram semen quality by luteolin enrichment during cold preservation
<p>The present experiment aimed to investigate the probable protective role of luteolin (Lut) in ram spermatozoa kinematics and the oxidative/anti-oxidative/nitrosative status of semen during cold storage. Ejaculates were collected from five Qezel rams twice a week. Ejaculates were pooled, dil...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2024-03-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/67/123/2024/aab-67-123-2024.pdf |
| _version_ | 1797236780487933952 |
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| author | S. Khozein M. Eslami F. Farrokhi-Ardabili |
| author_facet | S. Khozein M. Eslami F. Farrokhi-Ardabili |
| author_sort | S. Khozein |
| collection | DOAJ |
| description | <p>The present experiment aimed to investigate the probable protective role of luteolin (Lut) in ram spermatozoa kinematics and the oxidative/anti-oxidative/nitrosative status of semen during cold storage. Ejaculates were collected from five Qezel rams twice a week. Ejaculates were pooled, diluted with Tris–egg yolk extender (negative control) or supplemented with 0 (control; received the solvent of luteolin), 4, 8 and 16 <span class="inline-formula">µ</span>m Lut. Kinematics parameters, viability and membrane functionality of spermatozoa were assessed. Furthermore, amounts of malondialdehyde (MDA) and anti-oxidant activity (AOA), superoxide dismutase activity (SOD) and total nitrate nitrite (TNN) were evaluated in the medium (diluent) and spermatozoa, separately, at 0, 24, 48 and 72 h after storage at 4 °C. Percentages of forward progressive motility and membrane integrity were significantly higher in 8 and 16 <span class="inline-formula">µ</span>m groups compared to control groups at 72 h (<span class="inline-formula"><i>P</i><i><</i>0.05</span>). No significant differences were observed in viability among groups during the study (<span class="inline-formula"><i>P</i><i>></i>0.05</span>). Lower MDA contents were observed in medium and spermatozoa of 8 and 16 <span class="inline-formula">µ</span>m treated groups compared to controls at 72 h (<span class="inline-formula"><i>P</i><i><</i>0.05</span>). In addition, higher AOA levels were observed in the medium of Lut-treated groups compared to controls at 48 and 72 h (<span class="inline-formula"><i>P</i><i><</i>0.05</span>). The activity of SOD was improved by luteolin addition. Luteolin enrichment did not affect TNN amounts. It seems that luteolin (at 8 and 16 <span class="inline-formula">µ</span>m) as a flavonoid protects the ram semen by its anti-oxidative properties and by reduction of lipid peroxidation following 48 and 72 h storage.</p> |
| first_indexed | 2024-04-24T17:09:18Z |
| format | Article |
| id | doaj.art-4342fd507d6b4e2eb51740b0c844d910 |
| institution | Directory Open Access Journal |
| issn | 0003-9438 2363-9822 |
| language | English |
| last_indexed | 2024-04-24T17:09:18Z |
| publishDate | 2024-03-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj.art-4342fd507d6b4e2eb51740b0c844d9102024-03-28T14:43:16ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222024-03-016712313210.5194/aab-67-123-2024Improvement of ram semen quality by luteolin enrichment during cold preservationS. Khozein0M. Eslami1F. Farrokhi-Ardabili2Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran<p>The present experiment aimed to investigate the probable protective role of luteolin (Lut) in ram spermatozoa kinematics and the oxidative/anti-oxidative/nitrosative status of semen during cold storage. Ejaculates were collected from five Qezel rams twice a week. Ejaculates were pooled, diluted with Tris–egg yolk extender (negative control) or supplemented with 0 (control; received the solvent of luteolin), 4, 8 and 16 <span class="inline-formula">µ</span>m Lut. Kinematics parameters, viability and membrane functionality of spermatozoa were assessed. Furthermore, amounts of malondialdehyde (MDA) and anti-oxidant activity (AOA), superoxide dismutase activity (SOD) and total nitrate nitrite (TNN) were evaluated in the medium (diluent) and spermatozoa, separately, at 0, 24, 48 and 72 h after storage at 4 °C. Percentages of forward progressive motility and membrane integrity were significantly higher in 8 and 16 <span class="inline-formula">µ</span>m groups compared to control groups at 72 h (<span class="inline-formula"><i>P</i><i><</i>0.05</span>). No significant differences were observed in viability among groups during the study (<span class="inline-formula"><i>P</i><i>></i>0.05</span>). Lower MDA contents were observed in medium and spermatozoa of 8 and 16 <span class="inline-formula">µ</span>m treated groups compared to controls at 72 h (<span class="inline-formula"><i>P</i><i><</i>0.05</span>). In addition, higher AOA levels were observed in the medium of Lut-treated groups compared to controls at 48 and 72 h (<span class="inline-formula"><i>P</i><i><</i>0.05</span>). The activity of SOD was improved by luteolin addition. Luteolin enrichment did not affect TNN amounts. It seems that luteolin (at 8 and 16 <span class="inline-formula">µ</span>m) as a flavonoid protects the ram semen by its anti-oxidative properties and by reduction of lipid peroxidation following 48 and 72 h storage.</p>https://aab.copernicus.org/articles/67/123/2024/aab-67-123-2024.pdf |
| spellingShingle | S. Khozein M. Eslami F. Farrokhi-Ardabili Improvement of ram semen quality by luteolin enrichment during cold preservation Archives Animal Breeding |
| title | Improvement of ram semen quality by luteolin enrichment during cold preservation |
| title_full | Improvement of ram semen quality by luteolin enrichment during cold preservation |
| title_fullStr | Improvement of ram semen quality by luteolin enrichment during cold preservation |
| title_full_unstemmed | Improvement of ram semen quality by luteolin enrichment during cold preservation |
| title_short | Improvement of ram semen quality by luteolin enrichment during cold preservation |
| title_sort | improvement of ram semen quality by luteolin enrichment during cold preservation |
| url | https://aab.copernicus.org/articles/67/123/2024/aab-67-123-2024.pdf |
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