In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach.
BACKGROUND:The capacity of Aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. Until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-04-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC5393893?pdf=render |
| _version_ | 1818018765213794304 |
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| author | Frederic Faucon Thierry Gaude Isabelle Dusfour Vincent Navratil Vincent Corbel Waraporn Juntarajumnong Romain Girod Rodolphe Poupardin Frederic Boyer Stephane Reynaud Jean-Philippe David |
| author_facet | Frederic Faucon Thierry Gaude Isabelle Dusfour Vincent Navratil Vincent Corbel Waraporn Juntarajumnong Romain Girod Rodolphe Poupardin Frederic Boyer Stephane Reynaud Jean-Philippe David |
| author_sort | Frederic Faucon |
| collection | DOAJ |
| description | BACKGROUND:The capacity of Aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. Until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. Metabolic resistance to pyrethroids is common in Aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robust genomic markers. METHODOLOGY/PRINCIPAL FINDINGS:In an attempt to identify the genomic bases of metabolic resistance to deltamethrin, multiple resistant and susceptible populations originating from various continents were compared using both RNA-seq and a targeted DNA-seq approach focused on the upstream regions of detoxification genes. Multiple detoxification enzymes were over transcribed in resistant populations, frequently associated with an increase in their gene copy number. Targeted sequencing identified potential promoter variations associated with their over transcription. Non-synonymous variations affecting detoxification enzymes were also identified in resistant populations. CONCLUSION /SIGNIFICANCE:This study not only confirmed the role of gene copy number variations as a frequent cause of the over expression of detoxification enzymes associated with insecticide resistance in Aedes aegypti but also identified novel genomic resistance markers potentially associated with their cis-regulation and modifications of their protein structure conformation. As for gene transcription data, polymorphism patterns were frequently conserved within regions but differed among continents confirming the selection of different resistance factors worldwide. Overall, this study paves the way of the identification of a comprehensive set of genomic markers for monitoring the spatio-temporal dynamics of the variety of insecticide resistance mechanisms in Aedes aegypti. |
| first_indexed | 2024-04-14T07:44:05Z |
| format | Article |
| id | doaj.art-4344d7c54916420a949ad86e49a1a596 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-14T07:44:05Z |
| publishDate | 2017-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-4344d7c54916420a949ad86e49a1a5962022-12-22T02:05:24ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352017-04-01114e000552610.1371/journal.pntd.0005526In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach.Frederic FauconThierry GaudeIsabelle DusfourVincent NavratilVincent CorbelWaraporn JuntarajumnongRomain GirodRodolphe PoupardinFrederic BoyerStephane ReynaudJean-Philippe DavidBACKGROUND:The capacity of Aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. Until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. Metabolic resistance to pyrethroids is common in Aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robust genomic markers. METHODOLOGY/PRINCIPAL FINDINGS:In an attempt to identify the genomic bases of metabolic resistance to deltamethrin, multiple resistant and susceptible populations originating from various continents were compared using both RNA-seq and a targeted DNA-seq approach focused on the upstream regions of detoxification genes. Multiple detoxification enzymes were over transcribed in resistant populations, frequently associated with an increase in their gene copy number. Targeted sequencing identified potential promoter variations associated with their over transcription. Non-synonymous variations affecting detoxification enzymes were also identified in resistant populations. CONCLUSION /SIGNIFICANCE:This study not only confirmed the role of gene copy number variations as a frequent cause of the over expression of detoxification enzymes associated with insecticide resistance in Aedes aegypti but also identified novel genomic resistance markers potentially associated with their cis-regulation and modifications of their protein structure conformation. As for gene transcription data, polymorphism patterns were frequently conserved within regions but differed among continents confirming the selection of different resistance factors worldwide. Overall, this study paves the way of the identification of a comprehensive set of genomic markers for monitoring the spatio-temporal dynamics of the variety of insecticide resistance mechanisms in Aedes aegypti.http://europepmc.org/articles/PMC5393893?pdf=render |
| spellingShingle | Frederic Faucon Thierry Gaude Isabelle Dusfour Vincent Navratil Vincent Corbel Waraporn Juntarajumnong Romain Girod Rodolphe Poupardin Frederic Boyer Stephane Reynaud Jean-Philippe David In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach. PLoS Neglected Tropical Diseases |
| title | In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach. |
| title_full | In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach. |
| title_fullStr | In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach. |
| title_full_unstemmed | In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach. |
| title_short | In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach. |
| title_sort | in the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito aedes aegypti an integrated next generation sequencing approach |
| url | http://europepmc.org/articles/PMC5393893?pdf=render |
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