Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum
Vitamin D deficiency is associated with various diseases such as obesity, digestive problems, osteoporosis, depression, and infections, and has therefore emerged as a topic of great interest in public healthcare. The quantitative assessment of 25-hydroxyvitamin D (25-OH VD) in human serum may accura...
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| Format: | Article |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1326254/full |
| _version_ | 1827576558002372608 |
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| author | Jianfa Wang Guoshao Sun Fang Li Zhi Zhu Lei Sun Pengju Lv Han Yue |
| author_facet | Jianfa Wang Guoshao Sun Fang Li Zhi Zhu Lei Sun Pengju Lv Han Yue |
| author_sort | Jianfa Wang |
| collection | DOAJ |
| description | Vitamin D deficiency is associated with various diseases such as obesity, digestive problems, osteoporosis, depression, and infections, and has therefore emerged as a topic of great interest in public healthcare. The quantitative assessment of 25-hydroxyvitamin D (25-OH VD) in human serum may accurately reflect the nutritional status of vitamin D in the human body, which is significant for the prevention and treatment of vitamin D-deficient patients. In this study, we developed an assay for quantitative detection of 25-OH VD based on the 25-OH VD monoclonal antibody (mAb), and identified the optimal process parameters. The following process settings were found to be suitable for the test strips: pH of 7.6, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) ratio of 1:2000, and the anti-25-OH VD mAb ratio was 1:8. The equilibration time of the immune dynamic assay was 15 min. Under optimal conditions, the quantum dot nanoparticle-based fluorescent immunochromatographic assay (QDs-FICA) exhibited dynamic linear detection of 25-OH VD in PBS, from 5 ng/mL to 100 ng/mL, and the strip quantitative curve could be represented by the following regression equation: y = −0.02088 logx)+1.444 (R2 = 0.9050). The IC50 of the QDs-FICA was 39.6 ± 1.33 ng/mL. The specificity of the QDs-FICA was evaluated by running several structurally related analogues, including 25-OH VD2, 25-OH VD3, 1,25-OH2VD3, 1,25-OH2VD2, VD2, and VD3. The coefficients of variation were all below 10%. The shelf life of the test strips in this study was about 160 days at room temperature. Briefly, this study is the first to perform QDs-FICA for the rapid visual and quantitative detection of 25-OH VD, with great potential significance for clinical diagnosis of vitamin D-associated diseases. |
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| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T21:13:14Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-4349f14807d04382bbb51767b10ee6832023-12-22T04:29:28ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-12-011110.3389/fbioe.2023.13262541326254Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serumJianfa Wang0Guoshao Sun1Fang Li2Zhi Zhu3Lei Sun4Pengju Lv5Han Yue6Department of Orthopedics, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Orthopedics, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Orthopedics, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Orthopedics, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou, Henan, ChinaCenter of Stem Cell and Regenerative Medicine, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou, Henan, ChinaCenter of Stem Cell and Regenerative Medicine, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou, Henan, ChinaCenter of Stem Cell and Regenerative Medicine, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou, Henan, ChinaVitamin D deficiency is associated with various diseases such as obesity, digestive problems, osteoporosis, depression, and infections, and has therefore emerged as a topic of great interest in public healthcare. The quantitative assessment of 25-hydroxyvitamin D (25-OH VD) in human serum may accurately reflect the nutritional status of vitamin D in the human body, which is significant for the prevention and treatment of vitamin D-deficient patients. In this study, we developed an assay for quantitative detection of 25-OH VD based on the 25-OH VD monoclonal antibody (mAb), and identified the optimal process parameters. The following process settings were found to be suitable for the test strips: pH of 7.6, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) ratio of 1:2000, and the anti-25-OH VD mAb ratio was 1:8. The equilibration time of the immune dynamic assay was 15 min. Under optimal conditions, the quantum dot nanoparticle-based fluorescent immunochromatographic assay (QDs-FICA) exhibited dynamic linear detection of 25-OH VD in PBS, from 5 ng/mL to 100 ng/mL, and the strip quantitative curve could be represented by the following regression equation: y = −0.02088 logx)+1.444 (R2 = 0.9050). The IC50 of the QDs-FICA was 39.6 ± 1.33 ng/mL. The specificity of the QDs-FICA was evaluated by running several structurally related analogues, including 25-OH VD2, 25-OH VD3, 1,25-OH2VD3, 1,25-OH2VD2, VD2, and VD3. The coefficients of variation were all below 10%. The shelf life of the test strips in this study was about 160 days at room temperature. Briefly, this study is the first to perform QDs-FICA for the rapid visual and quantitative detection of 25-OH VD, with great potential significance for clinical diagnosis of vitamin D-associated diseases.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1326254/full25-hydroxyvitamin DZnCdSe/ZnSquantum dotsfluorescence immunochromatographic assayquantitative detection |
| spellingShingle | Jianfa Wang Guoshao Sun Fang Li Zhi Zhu Lei Sun Pengju Lv Han Yue Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum Frontiers in Bioengineering and Biotechnology 25-hydroxyvitamin D ZnCdSe/ZnS quantum dots fluorescence immunochromatographic assay quantitative detection |
| title | Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum |
| title_full | Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum |
| title_fullStr | Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum |
| title_full_unstemmed | Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum |
| title_short | Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum |
| title_sort | development of zncdse zns quantum dot based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins d in human serum |
| topic | 25-hydroxyvitamin D ZnCdSe/ZnS quantum dots fluorescence immunochromatographic assay quantitative detection |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1326254/full |
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