The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected Macrophages
Background and Objectives: Leishmaniasis is a disease produced by several species of protozoa of the Leishmania genus. As a therapeutic agent, garlic (Allium sativum) is one of the most popular plants in traditional medicinal systems and is increasingly becoming important in herbal alternative thera...
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| Format: | Article |
| Language: | fas |
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Qom University of Medical Sciences
2011-03-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
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| Online Access: | http://journal.muq.ac.ir/library/upload/article/af_254694390.17.12.pdf |
| _version_ | 1818269085826285568 |
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| author | Khadem Vatan Sh Bakhshayesh M Nobakht M Gharavi M.J Fani F |
| author_facet | Khadem Vatan Sh Bakhshayesh M Nobakht M Gharavi M.J Fani F |
| author_sort | Khadem Vatan Sh |
| collection | DOAJ |
| description | Background and Objectives: Leishmaniasis is a disease produced by several species of protozoa of the Leishmania genus. As a therapeutic agent, garlic (Allium sativum) is one of the most popular plants in traditional medicinal systems and is increasingly becoming important in herbal alternative therapy. The aim of the present study was to investigate the effects of aqueous garlic extract (AGE) on enhancing T-helper IL-10 and IL-12 cytokines in the culture via macrophages for engulfment and destruction of Leishmania. Methods: After proliferation of macrophages in culture and incubation with Leishmania during 72 hours, we added aqueaous garlic extract by several doses 9.25, 18.5, 37, 74 and 148µg/ml for 18, 24 and 48 hours. Trypan blue and MTT tests were carried out in order to test the safety of extracts and j744 cells treated with them, as well as cell viability. An enzyme-linked immunosorbant assay (ELISA) was performed on macrophages for interleukin IL-10 and IL-12. Results: The results suggested that garlic extract may provide effective therapy against Leishmania. Our results showed that dose of 37µg/ml for 48 hours of garlic extract destructed promastigots by IL-12 secreted from macrophages.Conclusion: Based on the results of this study, IL-12 is crucial for defense against parasitic pathogens. IL-12 administration differentially affected immune response to invading Leishmania parasites. |
| first_indexed | 2024-12-12T20:48:47Z |
| format | Article |
| id | doaj.art-434b9485782c410293cb14afc24307d3 |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-12T20:48:47Z |
| publishDate | 2011-03-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-434b9485782c410293cb14afc24307d32022-12-22T00:12:28ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752011-03-01518389The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected MacrophagesKhadem Vatan ShBakhshayesh MNobakht MGharavi M.JFani FBackground and Objectives: Leishmaniasis is a disease produced by several species of protozoa of the Leishmania genus. As a therapeutic agent, garlic (Allium sativum) is one of the most popular plants in traditional medicinal systems and is increasingly becoming important in herbal alternative therapy. The aim of the present study was to investigate the effects of aqueous garlic extract (AGE) on enhancing T-helper IL-10 and IL-12 cytokines in the culture via macrophages for engulfment and destruction of Leishmania. Methods: After proliferation of macrophages in culture and incubation with Leishmania during 72 hours, we added aqueaous garlic extract by several doses 9.25, 18.5, 37, 74 and 148µg/ml for 18, 24 and 48 hours. Trypan blue and MTT tests were carried out in order to test the safety of extracts and j744 cells treated with them, as well as cell viability. An enzyme-linked immunosorbant assay (ELISA) was performed on macrophages for interleukin IL-10 and IL-12. Results: The results suggested that garlic extract may provide effective therapy against Leishmania. Our results showed that dose of 37µg/ml for 48 hours of garlic extract destructed promastigots by IL-12 secreted from macrophages.Conclusion: Based on the results of this study, IL-12 is crucial for defense against parasitic pathogens. IL-12 administration differentially affected immune response to invading Leishmania parasites.http://journal.muq.ac.ir/library/upload/article/af_254694390.17.12.pdfLeishmania MajorGarlic (Allium sativum)Interleukin-12Interleukin-10Macrophages |
| spellingShingle | Khadem Vatan Sh Bakhshayesh M Nobakht M Gharavi M.J Fani F The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected Macrophages Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum Leishmania Major Garlic (Allium sativum) Interleukin-12 Interleukin-10 Macrophages |
| title | The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected Macrophages |
| title_full | The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected Macrophages |
| title_fullStr | The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected Macrophages |
| title_full_unstemmed | The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected Macrophages |
| title_short | The Role of Aqueous Garlic Extract (Allium Sativum) on Interleukin 10 and 12 in Leishmania Infected Macrophages |
| title_sort | role of aqueous garlic extract allium sativum on interleukin 10 and 12 in leishmania infected macrophages |
| topic | Leishmania Major Garlic (Allium sativum) Interleukin-12 Interleukin-10 Macrophages |
| url | http://journal.muq.ac.ir/library/upload/article/af_254694390.17.12.pdf |
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