Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different Sources
The aim of the present study was to evaluate the differential expression of plasma proteins in broiler chickens supplemented with different sources (sulfates and hydroxychlorides) and levels of copper (15 and 150 mg kg<sup>−1</sup>) and manganese (80 and 120 mg kg<sup>−1</sup>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/24/8155 |
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| author | Renata Aparecida Martins Andrey Sávio de Almeida Assunção José Cavalcante Souza Vieira Leone Campos Rocha Priscila Michelin Groff Urayama Marília Afonso Rabelo Buzalaf José Roberto Sartori Pedro de Magalhães Padilha |
| author_facet | Renata Aparecida Martins Andrey Sávio de Almeida Assunção José Cavalcante Souza Vieira Leone Campos Rocha Priscila Michelin Groff Urayama Marília Afonso Rabelo Buzalaf José Roberto Sartori Pedro de Magalhães Padilha |
| author_sort | Renata Aparecida Martins |
| collection | DOAJ |
| description | The aim of the present study was to evaluate the differential expression of plasma proteins in broiler chickens supplemented with different sources (sulfates and hydroxychlorides) and levels of copper (15 and 150 mg kg<sup>−1</sup>) and manganese (80 and 120 mg kg<sup>−1</sup>). For this, plasma samples from 40 broiler chickens were used, divided into four experimental groups: S15-80 (15 ppm CuSO<sub>4</sub> and 80 ppm MnSO<sub>4</sub>), S150-120 (150 ppm CuSO<sub>4</sub> and 120 ppm MnSO<sub>4</sub>), H15-80 (15 ppm Cu(OH)Cl and 80 ppm Mn(OH)Cl), and H150-120 (150 ppm Cu(OH)Cl and 120 ppm Mn(OH)Cl). From plasma samples obtained from each bird from the same treatment, four pools were made considering 10 birds per group. Plasma proteome fractionation was performed by 2D-PAGE. Concentrations of the studied minerals were also evaluated in both plasma and protein pellet samples. A higher concentration of Cu and Mn was observed in the plasma and protein pellets of groups that received higher mineral supplementation levels compared to those receiving lower levels. Mn concentrations were higher in plasma and protein pellets of the hydroxychloride-supplemented groups than the sulfate-supplemented groups. Analysis of the gels revealed a total of 40 differentially expressed spots among the four treatments. Supplementation with different sources of minerals, particularly at higher levels, resulted in changes in protein regulation, suggesting a potential imbalance in homeostasis. |
| first_indexed | 2024-03-08T20:29:49Z |
| format | Article |
| id | doaj.art-434c031369074f3a928fc5e687af0499 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T20:29:49Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-434c031369074f3a928fc5e687af04992023-12-22T14:28:04ZengMDPI AGMolecules1420-30492023-12-012824815510.3390/molecules28248155Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different SourcesRenata Aparecida Martins0Andrey Sávio de Almeida Assunção1José Cavalcante Souza Vieira2Leone Campos Rocha3Priscila Michelin Groff Urayama4Marília Afonso Rabelo Buzalaf5José Roberto Sartori6Pedro de Magalhães Padilha7School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilInstitute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-693, SP, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilBauru School of Dentistry, University of São Paulo (USP), Bauru 17012-901, SP, BrazilSchool of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilInstitute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-693, SP, BrazilThe aim of the present study was to evaluate the differential expression of plasma proteins in broiler chickens supplemented with different sources (sulfates and hydroxychlorides) and levels of copper (15 and 150 mg kg<sup>−1</sup>) and manganese (80 and 120 mg kg<sup>−1</sup>). For this, plasma samples from 40 broiler chickens were used, divided into four experimental groups: S15-80 (15 ppm CuSO<sub>4</sub> and 80 ppm MnSO<sub>4</sub>), S150-120 (150 ppm CuSO<sub>4</sub> and 120 ppm MnSO<sub>4</sub>), H15-80 (15 ppm Cu(OH)Cl and 80 ppm Mn(OH)Cl), and H150-120 (150 ppm Cu(OH)Cl and 120 ppm Mn(OH)Cl). From plasma samples obtained from each bird from the same treatment, four pools were made considering 10 birds per group. Plasma proteome fractionation was performed by 2D-PAGE. Concentrations of the studied minerals were also evaluated in both plasma and protein pellet samples. A higher concentration of Cu and Mn was observed in the plasma and protein pellets of groups that received higher mineral supplementation levels compared to those receiving lower levels. Mn concentrations were higher in plasma and protein pellets of the hydroxychloride-supplemented groups than the sulfate-supplemented groups. Analysis of the gels revealed a total of 40 differentially expressed spots among the four treatments. Supplementation with different sources of minerals, particularly at higher levels, resulted in changes in protein regulation, suggesting a potential imbalance in homeostasis.https://www.mdpi.com/1420-3049/28/24/8155minerals supplementation sourcescopper and manganese bioavailabilitybroiler nutritionproteomicsmetalloproteomics |
| spellingShingle | Renata Aparecida Martins Andrey Sávio de Almeida Assunção José Cavalcante Souza Vieira Leone Campos Rocha Priscila Michelin Groff Urayama Marília Afonso Rabelo Buzalaf José Roberto Sartori Pedro de Magalhães Padilha Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different Sources Molecules minerals supplementation sources copper and manganese bioavailability broiler nutrition proteomics metalloproteomics |
| title | Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different Sources |
| title_full | Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different Sources |
| title_fullStr | Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different Sources |
| title_full_unstemmed | Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different Sources |
| title_short | Proteomic Study of Broiler Plasma Supplemented with Different Levels of Copper and Manganese from Different Sources |
| title_sort | proteomic study of broiler plasma supplemented with different levels of copper and manganese from different sources |
| topic | minerals supplementation sources copper and manganese bioavailability broiler nutrition proteomics metalloproteomics |
| url | https://www.mdpi.com/1420-3049/28/24/8155 |
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