Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA Network
BackgroundOvarian cancer (OC), one of the most prevalent gynecological malignancies, is characterized by late detection and dismal prognosis. Recent studies show that long non-coding RNAs (lncRNAs) in competitive endogenous RNA (ceRNA) networks influence immune infiltration and cancer prognosis. How...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2020.566497/full |
| _version_ | 1819298609673273344 |
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| author | Huisheng Liang Huisheng Liang Yuquan Bai Hailong Wang Hailong Wang Xiangjun Yang |
| author_facet | Huisheng Liang Huisheng Liang Yuquan Bai Hailong Wang Hailong Wang Xiangjun Yang |
| author_sort | Huisheng Liang |
| collection | DOAJ |
| description | BackgroundOvarian cancer (OC), one of the most prevalent gynecological malignancies, is characterized by late detection and dismal prognosis. Recent studies show that long non-coding RNAs (lncRNAs) in competitive endogenous RNA (ceRNA) networks influence immune infiltration and cancer prognosis. However, the function of lncRNA in OC immune infiltration and prognosis remains unclear.MethodsTranscriptomes of 378 OC samples and clinical data were retrieved from the TCGA repository. Modules related to immune cells were identified using weighted gene co-expression network analysis (WGCNA). Functional enrichment analysis and survival analysis were then performed for the identification of immune-related lncRNAs in the brown module using Cox regression model. Finally, a ceRNA network was constructed by using the lncRNAs and mRNAs from the brown module.ResultsWe found lncRNAs and mRNAs in the brown module to be significantly associated with immune cells in OC and identified 4 lncRNAs as potential OC prognostic markers. We further established that lncRNAs in the ceRNA network influence OC immune infiltration and prognosis by regulating miRNA, ultimately modulating mRNA levels.ConclusionWe have identified 4 lncRNAs as independent immune prognostic factors for OC. Furthermore, our findings offer novel insight into lncRNAs as OC immune and prognostic biomarkers. |
| first_indexed | 2024-12-24T05:32:37Z |
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| id | doaj.art-434c70e422c847ff9c2b8ce88c524385 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-24T05:32:37Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-434c70e422c847ff9c2b8ce88c5243852022-12-21T17:13:08ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-02-011110.3389/fgene.2020.566497566497Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA NetworkHuisheng Liang0Huisheng Liang1Yuquan Bai2Hailong Wang3Hailong Wang4Xiangjun Yang5Department of Gynecology and Obstetrics, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen, ChinaOrgan Transplantation Institute, School of Medicine, Xiamen University, Xiamen, ChinaDepartment of Thoracic Surgery, West China Hospital of Sichuan University, Chengdu, ChinaOrgan Transplantation Institute, School of Medicine, Xiamen University, Xiamen, ChinaDepartment of Basic Medicine, School of Medicine, Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen, ChinaBackgroundOvarian cancer (OC), one of the most prevalent gynecological malignancies, is characterized by late detection and dismal prognosis. Recent studies show that long non-coding RNAs (lncRNAs) in competitive endogenous RNA (ceRNA) networks influence immune infiltration and cancer prognosis. However, the function of lncRNA in OC immune infiltration and prognosis remains unclear.MethodsTranscriptomes of 378 OC samples and clinical data were retrieved from the TCGA repository. Modules related to immune cells were identified using weighted gene co-expression network analysis (WGCNA). Functional enrichment analysis and survival analysis were then performed for the identification of immune-related lncRNAs in the brown module using Cox regression model. Finally, a ceRNA network was constructed by using the lncRNAs and mRNAs from the brown module.ResultsWe found lncRNAs and mRNAs in the brown module to be significantly associated with immune cells in OC and identified 4 lncRNAs as potential OC prognostic markers. We further established that lncRNAs in the ceRNA network influence OC immune infiltration and prognosis by regulating miRNA, ultimately modulating mRNA levels.ConclusionWe have identified 4 lncRNAs as independent immune prognostic factors for OC. Furthermore, our findings offer novel insight into lncRNAs as OC immune and prognostic biomarkers.https://www.frontiersin.org/articles/10.3389/fgene.2020.566497/fullimmune cell abundanceweighted gene co-expression network analyseslong noncoding RNAceRNA network analysisovarian cancer |
| spellingShingle | Huisheng Liang Huisheng Liang Yuquan Bai Hailong Wang Hailong Wang Xiangjun Yang Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA Network Frontiers in Genetics immune cell abundance weighted gene co-expression network analyses long noncoding RNA ceRNA network analysis ovarian cancer |
| title | Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA Network |
| title_full | Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA Network |
| title_fullStr | Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA Network |
| title_full_unstemmed | Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA Network |
| title_short | Identification of LncRNA Prognostic Markers for Ovarian Cancer by Integration of Co-expression and CeRNA Network |
| title_sort | identification of lncrna prognostic markers for ovarian cancer by integration of co expression and cerna network |
| topic | immune cell abundance weighted gene co-expression network analyses long noncoding RNA ceRNA network analysis ovarian cancer |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2020.566497/full |
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