Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes Injury
Pelargonidin-<i>3</i>-<i>O</i>-glucoside (Pg) is a well-known anthocyanin derivative possessing potential biological activity. Nonetheless, the bioactivity of Pg is limited due to instability in the physiological environment. Functionalized nanoliposomes using chitosan and/or...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/4/623 |
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| author | Naymul Karim Mohammad Rezaul Islam Shishir Yuting Li Ould Yahia Zineb Jianling Mo Jitbanjong Tangpong Wei Chen |
| author_facet | Naymul Karim Mohammad Rezaul Islam Shishir Yuting Li Ould Yahia Zineb Jianling Mo Jitbanjong Tangpong Wei Chen |
| author_sort | Naymul Karim |
| collection | DOAJ |
| description | Pelargonidin-<i>3</i>-<i>O</i>-glucoside (Pg) is a well-known anthocyanin derivative possessing potential biological activity. Nonetheless, the bioactivity of Pg is limited due to instability in the physiological environment. Functionalized nanoliposomes using chitosan and/or pectin coating is an excellent carrier system for nanoencapsulation of food bioactive compounds such as Pg. Therefore, this study aimed to investigate the protective effect of Pg-loaded pectin–chitosan coated nanoliposomes against palmitic acid (PA)-induced hepatocytes injury in L02 cells. Firstly, Pg-loaded pectin–chitosan coated nanoliposomes were characterized using the DLS, HPLC, TEM, and cellular uptake study in L02 cells. Thereafter, we assayed the protective effect against PA-induced lipotoxicity, ROS and O<sub>2</sub><sup>•−</sup> generation, mitochondrial dysfunction (MMP), and GSH depletion. Results showed that Pg-loaded nanoliposomes significantly reduced the PA-induced L02 cells toxicity via suppressing ROS production, O<sub>2</sub><sup>•−</sup> generation, MMP collapse, and GSH reduction, whereas the free-Pg samples were not effective. On the contrary, the chitosan and/or pectin coated nanoliposomes showed higher results compared to coating-free nanoliposomes. Altogether, the results of our study ensured that Pg-loaded pectin–chitosan coated nanoliposomes was capable of reducing PA-induced hepatocytes injury. Thus, pectin–chitosan coated nanoliposomes can be useful for hepatocellular delivery of hydrophilic compounds with greater biological activity. |
| first_indexed | 2024-03-09T11:14:47Z |
| format | Article |
| id | doaj.art-434d70b145614ed692f6dd37e1ee9c6e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:14:47Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-434d70b145614ed692f6dd37e1ee9c6e2023-12-01T00:33:52ZengMDPI AGAntioxidants2076-39212022-03-0111462310.3390/antiox11040623Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes InjuryNaymul Karim0Mohammad Rezaul Islam Shishir1Yuting Li2Ould Yahia Zineb3Jianling Mo4Jitbanjong Tangpong5Wei Chen6Department of Traditional Chinese Medicine, School of Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, ChinaDepartment of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, ChinaDepartment of Traditional Chinese Medicine, School of Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, ChinaBiomedical Sciences, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, ThailandDepartment of Traditional Chinese Medicine, School of Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, ChinaPelargonidin-<i>3</i>-<i>O</i>-glucoside (Pg) is a well-known anthocyanin derivative possessing potential biological activity. Nonetheless, the bioactivity of Pg is limited due to instability in the physiological environment. Functionalized nanoliposomes using chitosan and/or pectin coating is an excellent carrier system for nanoencapsulation of food bioactive compounds such as Pg. Therefore, this study aimed to investigate the protective effect of Pg-loaded pectin–chitosan coated nanoliposomes against palmitic acid (PA)-induced hepatocytes injury in L02 cells. Firstly, Pg-loaded pectin–chitosan coated nanoliposomes were characterized using the DLS, HPLC, TEM, and cellular uptake study in L02 cells. Thereafter, we assayed the protective effect against PA-induced lipotoxicity, ROS and O<sub>2</sub><sup>•−</sup> generation, mitochondrial dysfunction (MMP), and GSH depletion. Results showed that Pg-loaded nanoliposomes significantly reduced the PA-induced L02 cells toxicity via suppressing ROS production, O<sub>2</sub><sup>•−</sup> generation, MMP collapse, and GSH reduction, whereas the free-Pg samples were not effective. On the contrary, the chitosan and/or pectin coated nanoliposomes showed higher results compared to coating-free nanoliposomes. Altogether, the results of our study ensured that Pg-loaded pectin–chitosan coated nanoliposomes was capable of reducing PA-induced hepatocytes injury. Thus, pectin–chitosan coated nanoliposomes can be useful for hepatocellular delivery of hydrophilic compounds with greater biological activity.https://www.mdpi.com/2076-3921/11/4/623liposomeschitosanpectinpelargonidin-<i>3</i>-<i>O</i>-glucosidepalmitic acidlipotoxicity |
| spellingShingle | Naymul Karim Mohammad Rezaul Islam Shishir Yuting Li Ould Yahia Zineb Jianling Mo Jitbanjong Tangpong Wei Chen Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes Injury Antioxidants liposomes chitosan pectin pelargonidin-<i>3</i>-<i>O</i>-glucoside palmitic acid lipotoxicity |
| title | Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes Injury |
| title_full | Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes Injury |
| title_fullStr | Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes Injury |
| title_full_unstemmed | Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes Injury |
| title_short | Pelargonidin-<i>3</i>-<i>O</i>-Glucoside Encapsulated Pectin-Chitosan-Nanoliposomes Recovers Palmitic Acid-Induced Hepatocytes Injury |
| title_sort | pelargonidin i 3 i i o i glucoside encapsulated pectin chitosan nanoliposomes recovers palmitic acid induced hepatocytes injury |
| topic | liposomes chitosan pectin pelargonidin-<i>3</i>-<i>O</i>-glucoside palmitic acid lipotoxicity |
| url | https://www.mdpi.com/2076-3921/11/4/623 |
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