Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation
Lactic acid bacteria are one of the bioresources that can promote the host’s health and have potential therapeutic applications. This study aimed to evaluate the probiotic properties of novel <i>Lactiplantibacillus plantarum</i> NWAFU-BISO-BS29 isolated in vitro from traditional Chinese...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/23/3766 |
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| author | Mohamedelfatieh Ismael Yaxin Gu Yanlong Cui Tao Wang Fangfang Yue Yanting Qin Xin Lü |
| author_facet | Mohamedelfatieh Ismael Yaxin Gu Yanlong Cui Tao Wang Fangfang Yue Yanting Qin Xin Lü |
| author_sort | Mohamedelfatieh Ismael |
| collection | DOAJ |
| description | Lactic acid bacteria are one of the bioresources that can promote the host’s health and have potential therapeutic applications. This study aimed to evaluate the probiotic properties of novel <i>Lactiplantibacillus plantarum</i> NWAFU-BISO-BS29 isolated in vitro from traditional Chinese fermented milk, assess its safety, and study its interaction with the gut microbiota using a BALB/c mouse model. The findings reveal that this strain had a high tolerance to gastric acidity (64.4%) and bile salts (19.83–87.92%) with remarkable auto-aggregation and co-aggregation abilities (33.01–83.96%), respectively. Furthermore, it lowered the cholesterol levels in dead cells (44.02%) and live cells (34.95%) and produced short-chain fatty acids (SCFAs). Likewise, it showed good antioxidant properties and strong antipathogen activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> with inhibition zones at 21 and 25 mm, respectively. The safety assessment results indicate that all of the virulence factor genes were not detected in the whole DNA; additionally, no hemolysis or resistance to antibiotics commonly used in food and feed was observed. Interestingly, the 16S rRNA gene sequencing of the mouse gut microbiota showed a marked alteration in the microbial composition of the administrated group, with a noticeable increase in Firmicutes, Patescibacteria, Campylobacterota, Deferribacterota, Proteobacteria, and Cyanobacteria at the phylum level. The modulation of gut microbial diversity significantly improved the production of SCFCs due to the abundance of lactobacillus genera, which was consistent with the functional gene predictive analysis and is believed to have health-promoting properties. Based on these results, our novel strain is considered a safe and good probiotic and could hold high potential to be used as a starter culture or to safely supplement functional foods as a probiotic and may provide new insights into therapeutic interventions. |
| first_indexed | 2024-03-09T17:47:19Z |
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| language | English |
| last_indexed | 2024-03-09T17:47:19Z |
| publishDate | 2022-11-01 |
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| series | Foods |
| spelling | doaj.art-434e94761c57449b9f158dbc468ed8102023-11-24T10:58:13ZengMDPI AGFoods2304-81582022-11-011123376610.3390/foods11233766Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota RegulationMohamedelfatieh Ismael0Yaxin Gu1Yanlong Cui2Tao Wang3Fangfang Yue4Yanting Qin5Xin Lü6College of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Food Science, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, ChinaLactic acid bacteria are one of the bioresources that can promote the host’s health and have potential therapeutic applications. This study aimed to evaluate the probiotic properties of novel <i>Lactiplantibacillus plantarum</i> NWAFU-BISO-BS29 isolated in vitro from traditional Chinese fermented milk, assess its safety, and study its interaction with the gut microbiota using a BALB/c mouse model. The findings reveal that this strain had a high tolerance to gastric acidity (64.4%) and bile salts (19.83–87.92%) with remarkable auto-aggregation and co-aggregation abilities (33.01–83.96%), respectively. Furthermore, it lowered the cholesterol levels in dead cells (44.02%) and live cells (34.95%) and produced short-chain fatty acids (SCFAs). Likewise, it showed good antioxidant properties and strong antipathogen activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> with inhibition zones at 21 and 25 mm, respectively. The safety assessment results indicate that all of the virulence factor genes were not detected in the whole DNA; additionally, no hemolysis or resistance to antibiotics commonly used in food and feed was observed. Interestingly, the 16S rRNA gene sequencing of the mouse gut microbiota showed a marked alteration in the microbial composition of the administrated group, with a noticeable increase in Firmicutes, Patescibacteria, Campylobacterota, Deferribacterota, Proteobacteria, and Cyanobacteria at the phylum level. The modulation of gut microbial diversity significantly improved the production of SCFCs due to the abundance of lactobacillus genera, which was consistent with the functional gene predictive analysis and is believed to have health-promoting properties. Based on these results, our novel strain is considered a safe and good probiotic and could hold high potential to be used as a starter culture or to safely supplement functional foods as a probiotic and may provide new insights into therapeutic interventions.https://www.mdpi.com/2304-8158/11/23/3766probiotic<i>Lactiplantibacillus plantarum</i>virulence genesantioxidantshemolytic activitycholesterol-lowering |
| spellingShingle | Mohamedelfatieh Ismael Yaxin Gu Yanlong Cui Tao Wang Fangfang Yue Yanting Qin Xin Lü Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation Foods probiotic <i>Lactiplantibacillus plantarum</i> virulence genes antioxidants hemolytic activity cholesterol-lowering |
| title | Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation |
| title_full | Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation |
| title_fullStr | Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation |
| title_full_unstemmed | Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation |
| title_short | Probiotic of <i>Lactiplantibacillus plantarum</i> NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation |
| title_sort | probiotic of i lactiplantibacillus plantarum i nwafu bio bs29 isolated from chinese traditional fermented milk and its potential therapeutic applications based on gut microbiota regulation |
| topic | probiotic <i>Lactiplantibacillus plantarum</i> virulence genes antioxidants hemolytic activity cholesterol-lowering |
| url | https://www.mdpi.com/2304-8158/11/23/3766 |
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