Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood.
Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset of these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high content of e...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Public Library of Science (PLoS)
2014-12-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC4256170?pdf=render |
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| author | Chiaki Hori Takuya Ishida Kiyohiko Igarashi Masahiro Samejima Hitoshi Suzuki Emma Master Patricia Ferreira Francisco J Ruiz-Dueñas Benjamin Held Paulo Canessa Luis F Larrondo Monika Schmoll Irina S Druzhinina Christian P Kubicek Jill A Gaskell Phil Kersten Franz St John Jeremy Glasner Grzegorz Sabat Sandra Splinter BonDurant Khajamohiddin Syed Jagjit Yadav Anthony C Mgbeahuruike Andriy Kovalchuk Fred O Asiegbu Gerald Lackner Dirk Hoffmeister Jorge Rencoret Ana Gutiérrez Hui Sun Erika Lindquist Kerrie Barry Robert Riley Igor V Grigoriev Bernard Henrissat Ursula Kües Randy M Berka Angel T Martínez Sarah F Covert Robert A Blanchette Daniel Cullen |
| author_facet | Chiaki Hori Takuya Ishida Kiyohiko Igarashi Masahiro Samejima Hitoshi Suzuki Emma Master Patricia Ferreira Francisco J Ruiz-Dueñas Benjamin Held Paulo Canessa Luis F Larrondo Monika Schmoll Irina S Druzhinina Christian P Kubicek Jill A Gaskell Phil Kersten Franz St John Jeremy Glasner Grzegorz Sabat Sandra Splinter BonDurant Khajamohiddin Syed Jagjit Yadav Anthony C Mgbeahuruike Andriy Kovalchuk Fred O Asiegbu Gerald Lackner Dirk Hoffmeister Jorge Rencoret Ana Gutiérrez Hui Sun Erika Lindquist Kerrie Barry Robert Riley Igor V Grigoriev Bernard Henrissat Ursula Kües Randy M Berka Angel T Martínez Sarah F Covert Robert A Blanchette Daniel Cullen |
| author_sort | Chiaki Hori |
| collection | DOAJ |
| description | Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset of these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high content of extractives, which retards the establishment of other fungal species. The mechanism(s) by which P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report the annotated P. gigantea genome and compare profiles of its transcriptome and secretome when cultured on fresh-cut versus solvent-extracted loblolly pine wood. The P. gigantea genome contains a conventional repertoire of hydrolase genes involved in cellulose/hemicellulose degradation, whose patterns of expression were relatively unperturbed by the absence of extractives. The expression of genes typically ascribed to lignin degradation was also largely unaffected. In contrast, genes likely involved in the transformation and detoxification of wood extractives were highly induced in its presence. Their products included an ABC transporter, lipases, cytochrome P450s, glutathione S-transferase and aldehyde dehydrogenase. Other regulated genes of unknown function and several constitutively expressed genes are also likely involved in P. gigantea's extractives metabolism. These results contribute to our fundamental understanding of pioneer colonization of conifer wood and provide insight into the diverse chemistries employed by fungi in carbon cycling processes. |
| first_indexed | 2024-12-12T11:21:39Z |
| format | Article |
| id | doaj.art-434f069cf6ea415b92c60bff1801eac2 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-12T11:21:39Z |
| publishDate | 2014-12-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Genetics |
| spelling | doaj.art-434f069cf6ea415b92c60bff1801eac22022-12-22T00:26:00ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-12-011012e100475910.1371/journal.pgen.1004759Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood.Chiaki HoriTakuya IshidaKiyohiko IgarashiMasahiro SamejimaHitoshi SuzukiEmma MasterPatricia FerreiraFrancisco J Ruiz-DueñasBenjamin HeldPaulo CanessaLuis F LarrondoMonika SchmollIrina S DruzhininaChristian P KubicekJill A GaskellPhil KerstenFranz St JohnJeremy GlasnerGrzegorz SabatSandra Splinter BonDurantKhajamohiddin SyedJagjit YadavAnthony C MgbeahuruikeAndriy KovalchukFred O AsiegbuGerald LacknerDirk HoffmeisterJorge RencoretAna GutiérrezHui SunErika LindquistKerrie BarryRobert RileyIgor V GrigorievBernard HenrissatUrsula KüesRandy M BerkaAngel T MartínezSarah F CovertRobert A BlanchetteDaniel CullenCollectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset of these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high content of extractives, which retards the establishment of other fungal species. The mechanism(s) by which P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report the annotated P. gigantea genome and compare profiles of its transcriptome and secretome when cultured on fresh-cut versus solvent-extracted loblolly pine wood. The P. gigantea genome contains a conventional repertoire of hydrolase genes involved in cellulose/hemicellulose degradation, whose patterns of expression were relatively unperturbed by the absence of extractives. The expression of genes typically ascribed to lignin degradation was also largely unaffected. In contrast, genes likely involved in the transformation and detoxification of wood extractives were highly induced in its presence. Their products included an ABC transporter, lipases, cytochrome P450s, glutathione S-transferase and aldehyde dehydrogenase. Other regulated genes of unknown function and several constitutively expressed genes are also likely involved in P. gigantea's extractives metabolism. These results contribute to our fundamental understanding of pioneer colonization of conifer wood and provide insight into the diverse chemistries employed by fungi in carbon cycling processes.http://europepmc.org/articles/PMC4256170?pdf=render |
| spellingShingle | Chiaki Hori Takuya Ishida Kiyohiko Igarashi Masahiro Samejima Hitoshi Suzuki Emma Master Patricia Ferreira Francisco J Ruiz-Dueñas Benjamin Held Paulo Canessa Luis F Larrondo Monika Schmoll Irina S Druzhinina Christian P Kubicek Jill A Gaskell Phil Kersten Franz St John Jeremy Glasner Grzegorz Sabat Sandra Splinter BonDurant Khajamohiddin Syed Jagjit Yadav Anthony C Mgbeahuruike Andriy Kovalchuk Fred O Asiegbu Gerald Lackner Dirk Hoffmeister Jorge Rencoret Ana Gutiérrez Hui Sun Erika Lindquist Kerrie Barry Robert Riley Igor V Grigoriev Bernard Henrissat Ursula Kües Randy M Berka Angel T Martínez Sarah F Covert Robert A Blanchette Daniel Cullen Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. PLoS Genetics |
| title | Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. |
| title_full | Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. |
| title_fullStr | Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. |
| title_full_unstemmed | Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. |
| title_short | Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. |
| title_sort | analysis of the phlebiopsis gigantea genome transcriptome and secretome provides insight into its pioneer colonization strategies of wood |
| url | http://europepmc.org/articles/PMC4256170?pdf=render |
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