Multilocus Sequence Analysis and Detection of Copper Ion Resistance of <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> Causing Bacterial Blight in Cassava
Cassava (<i>Manihot esculenta</i> Crantz) is an important tropical tuber crop around the world. Cassava bacterial blight, caused by <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i>, is a key disease that influences cassava production worldwide. Between 2008 and 20...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/7/342 |
| Summary: | Cassava (<i>Manihot esculenta</i> Crantz) is an important tropical tuber crop around the world. Cassava bacterial blight, caused by <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i>, is a key disease that influences cassava production worldwide. Between 2008 and 2020, 50 <i>X. phaseoli</i> pv. <i>manihotis</i> strains were isolated from diseased plant samples or acquired from China, Uganda, Cambodia, Colombia, Malaysia, and Micronesia. Using multilocus sequence analysis, the genetic diversity of <i>X. phaseoli</i> pv. <i>manihotis</i> strains was evaluated. A neighbor-joining phylogenetic dendrogram was constructed based on partial sequences of five housekeeping genes (<i>atpD</i>-<i>dnaK</i>-<i>gyrB</i>-<i>efp</i>-<i>rpoD</i>). The strains clustered into three groups whose clusters were consistent with <i>atpD</i> and <i>RpoD</i> gene sequences. Group I contained 46 strains from China, Uganda, Cambodia, and Micronesia, and the other two groups were comprised of strains from Colombia and Malaysia, respectively. The resistance of all these strains to copper ion (Cu<sup>2+</sup>) was determined, the minimal inhibitory concentration was between 1.3 and 1.7 mM, and there was no significant difference between strains from different geographic region. During genome annotation of the <i>X. phaseoli</i> pv. <i>manihotis</i> strain CHN01, homologous gene clusters of <i>copLAB</i> and <i>xmeRSA</i> were identified. The predicted amino acid sequences of two gene clusters were highly homologous with the copper-resistant protein from <i>Xanthomonas</i> strains. <i>CopLAB</i> and <i>xmeRSA</i> were amplified from all these strains, suggesting that the regulation of copper resistance is associated with two distinct metabolic pathways. <i>CopLAB</i> and <i>xmeRSA</i> were highly conserved among strains from different geographic regions, possibly associated with other conserved function. |
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| ISSN: | 1467-3037 1467-3045 |