TRPV1 activation induces cell death of TM3 mouse Leydig cells
The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2021-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2580&vmd=Full |
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| author | Eun-Jin Kim Long Cao Dang Marie Merci Nyiramana Adrian S. Siregar Min-Seok Woo Chang-Woon Kim Dawon Kang |
| author_facet | Eun-Jin Kim Long Cao Dang Marie Merci Nyiramana Adrian S. Siregar Min-Seok Woo Chang-Woon Kim Dawon Kang |
| author_sort | Eun-Jin Kim |
| collection | DOAJ |
| description | The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicininduced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1. |
| first_indexed | 2024-12-22T20:06:48Z |
| format | Article |
| id | doaj.art-43506eabb124456b93db332d17d78047 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-22T20:06:48Z |
| publishDate | 2021-09-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-43506eabb124456b93db332d17d780472022-12-21T18:14:06ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632021-09-0136314515310.12750/JARB.36.3.145TRPV1 activation induces cell death of TM3 mouse Leydig cellsEun-Jin Kim0https://orcid.org/0000-0003-1068-9334Long Cao Dang1https://orcid.org/0000-0001-8203-4631Marie Merci Nyiramana2https://orcid.org/0000-0002-0018-8934Adrian S. Siregar3https://orcid.org/0000-0001-7069-4202Min-Seok Woo4https://orcid.org/0000-0002-0357-9666Chang-Woon Kim5https://orcid.org/0000-0002-6663-0764Dawon Kang6https://orcid.org/0000-0001-7402-7298Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 52727, KoreaDepartment of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 52727, KoreaDepartment of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 52727, KoreaDepartment of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 52727, KoreaDepartment of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 52727, KoreaDepartment of Obstetrics and Gynecology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon 51353, KoreaDepartment of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju 52727, KoreaThe role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicininduced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.https://www.e-jarb.org/journal/view.html?uid=2580&vmd=Fullapoptosisleydig cellsmicetestestrpv1 |
| spellingShingle | Eun-Jin Kim Long Cao Dang Marie Merci Nyiramana Adrian S. Siregar Min-Seok Woo Chang-Woon Kim Dawon Kang TRPV1 activation induces cell death of TM3 mouse Leydig cells Journal of Animal Reproduction and Biotechnology apoptosis leydig cells mice testes trpv1 |
| title | TRPV1 activation induces cell death of TM3 mouse Leydig cells |
| title_full | TRPV1 activation induces cell death of TM3 mouse Leydig cells |
| title_fullStr | TRPV1 activation induces cell death of TM3 mouse Leydig cells |
| title_full_unstemmed | TRPV1 activation induces cell death of TM3 mouse Leydig cells |
| title_short | TRPV1 activation induces cell death of TM3 mouse Leydig cells |
| title_sort | trpv1 activation induces cell death of tm3 mouse leydig cells |
| topic | apoptosis leydig cells mice testes trpv1 |
| url | https://www.e-jarb.org/journal/view.html?uid=2580&vmd=Full |
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