Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells
Summary: A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in micr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221012906 |
| _version_ | 1818825421283655680 |
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| author | Nam-Kyung Yu Daniel B. McClatchy Jolene K. Diedrich Sarah Romero Jun-Hyeok Choi Salvador Martínez-Bartolomé Claire M. Delahunty Alysson R. Muotri John R. Yates, III |
| author_facet | Nam-Kyung Yu Daniel B. McClatchy Jolene K. Diedrich Sarah Romero Jun-Hyeok Choi Salvador Martínez-Bartolomé Claire M. Delahunty Alysson R. Muotri John R. Yates, III |
| author_sort | Nam-Kyung Yu |
| collection | DOAJ |
| description | Summary: A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in microRNA biogenesis, diverse molecular roles have been recognized. In the nervous system, LIN28A is known to play critical roles in proliferation and differentiation of neural progenitor cells (NPCs). We profiled the endogenous LIN28A-interacting proteins in NPCs differentiated from human induced pluripotent stem (iPS) cells using immunoprecipitation and liquid chromatography-tandem mass spectrometry. We identified over 500 LIN28A-interacting proteins, including 156 RNA-independent interactors. Functions of these proteins span a wide range of gene regulatory processes. Prompted by the interactome data, we revealed that LIN28A may impact the subcellular distribution of its interactors and stress granule formation upon oxidative stress. Overall, our analysis opens multiple avenues for elaborating molecular roles and characteristics of LIN28A. |
| first_indexed | 2024-12-19T00:11:30Z |
| format | Article |
| id | doaj.art-4350b3b7587746e6bd8c1c81b0bf89e6 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-19T00:11:30Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-4350b3b7587746e6bd8c1c81b0bf89e62022-12-21T20:46:02ZengElsevieriScience2589-00422021-11-012411103321Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cellsNam-Kyung Yu0Daniel B. McClatchy1Jolene K. Diedrich2Sarah Romero3Jun-Hyeok Choi4Salvador Martínez-Bartolomé5Claire M. Delahunty6Alysson R. Muotri7John R. Yates, III8Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Pediatrics/Rady Children’s Hospital San Diego, Department of Cellular & Molecular Medicine, School of Medicine, University of California, San Diego, San Diego, CA 92037, USANeurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USADepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Pediatrics/Rady Children’s Hospital San Diego, Department of Cellular & Molecular Medicine, School of Medicine, University of California, San Diego, San Diego, CA 92037, USA; Stem Cell Program, Center for Academic Research and Training in Anthropogeny (CARTA), Archealization Center (ArchC), Kavli Institute for Brain and Mind, La Jolla, CA 92037, USADepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA; Corresponding authorSummary: A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in microRNA biogenesis, diverse molecular roles have been recognized. In the nervous system, LIN28A is known to play critical roles in proliferation and differentiation of neural progenitor cells (NPCs). We profiled the endogenous LIN28A-interacting proteins in NPCs differentiated from human induced pluripotent stem (iPS) cells using immunoprecipitation and liquid chromatography-tandem mass spectrometry. We identified over 500 LIN28A-interacting proteins, including 156 RNA-independent interactors. Functions of these proteins span a wide range of gene regulatory processes. Prompted by the interactome data, we revealed that LIN28A may impact the subcellular distribution of its interactors and stress granule formation upon oxidative stress. Overall, our analysis opens multiple avenues for elaborating molecular roles and characteristics of LIN28A.http://www.sciencedirect.com/science/article/pii/S2589004221012906Gene networkMolecular neuroscienceOmicsStem cells research |
| spellingShingle | Nam-Kyung Yu Daniel B. McClatchy Jolene K. Diedrich Sarah Romero Jun-Hyeok Choi Salvador Martínez-Bartolomé Claire M. Delahunty Alysson R. Muotri John R. Yates, III Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells iScience Gene network Molecular neuroscience Omics Stem cells research |
| title | Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
| title_full | Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
| title_fullStr | Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
| title_full_unstemmed | Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
| title_short | Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |
| title_sort | interactome analysis illustrates diverse gene regulatory processes associated with lin28a in human ips cell derived neural progenitor cells |
| topic | Gene network Molecular neuroscience Omics Stem cells research |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221012906 |
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