Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood Cells
Genotoxicity is the ability of specific substances to cause DNA damage, affecting development, physiology, and reproduction. This is often mediated by induction of oxidative stress. This <i>in vitro</i> study aims to test the ability of two antioxidants, ellagic acid (EA, 100 µM) and cur...
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MDPI AG
2021-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/17/8142 |
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| author | Filomena Mottola Renata Finelli Concetta Iovine Maria Carannante Marianna Santonastaso Lucia Rocco |
| author_facet | Filomena Mottola Renata Finelli Concetta Iovine Maria Carannante Marianna Santonastaso Lucia Rocco |
| author_sort | Filomena Mottola |
| collection | DOAJ |
| description | Genotoxicity is the ability of specific substances to cause DNA damage, affecting development, physiology, and reproduction. This is often mediated by induction of oxidative stress. This <i>in vitro</i> study aims to test the ability of two antioxidants, ellagic acid (EA, 100 µM) and curcumin (Cur, 40 µM) to protect zebrafish blood cells from the genotoxic action of benzene (10 µL/mL). Cells were treated for 30, 60, and 90 min with EA or Cur alone and in combination with benzene. The antigenotoxic role of antioxidants was evaluated in terms of cytotoxicity by trypan blue dye, genome stability by RAPD-PCR technique, DNA fragmentation and percentage of apoptotic cells using Comet and Diffusion assay, respectively. The results did not show statistical differences in terms of cell viability, genome stability, DNA damage and apoptosis between cells treated with antioxidants. When zebrafish blood cells were co-incubated with individual antioxidants and benzene, a significant improvement of these parameters was observed in comparison with cells incubated in benzene. Our results suggested that EA and Cur are able to protect zebrafish blood cells against DNA damage and apoptosis caused by mutagenic substance, and laid the foundation for future studies investigating their antigenotoxic potential in DNA oxidative damage therapy. |
| first_indexed | 2024-03-10T08:15:29Z |
| format | Article |
| id | doaj.art-4354c62cd12a4a7d868c960a7656b7a4 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T08:15:29Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4354c62cd12a4a7d868c960a7656b7a42023-11-22T10:22:14ZengMDPI AGApplied Sciences2076-34172021-09-011117814210.3390/app11178142Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood CellsFilomena Mottola0Renata Finelli1Concetta Iovine2Maria Carannante3Marianna Santonastaso4Lucia Rocco5Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyAmerican Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USADepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Woman, Child and General and Special Surgery, University of Campania “Luigi Vanvitelli”, 80138 Napoli, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyGenotoxicity is the ability of specific substances to cause DNA damage, affecting development, physiology, and reproduction. This is often mediated by induction of oxidative stress. This <i>in vitro</i> study aims to test the ability of two antioxidants, ellagic acid (EA, 100 µM) and curcumin (Cur, 40 µM) to protect zebrafish blood cells from the genotoxic action of benzene (10 µL/mL). Cells were treated for 30, 60, and 90 min with EA or Cur alone and in combination with benzene. The antigenotoxic role of antioxidants was evaluated in terms of cytotoxicity by trypan blue dye, genome stability by RAPD-PCR technique, DNA fragmentation and percentage of apoptotic cells using Comet and Diffusion assay, respectively. The results did not show statistical differences in terms of cell viability, genome stability, DNA damage and apoptosis between cells treated with antioxidants. When zebrafish blood cells were co-incubated with individual antioxidants and benzene, a significant improvement of these parameters was observed in comparison with cells incubated in benzene. Our results suggested that EA and Cur are able to protect zebrafish blood cells against DNA damage and apoptosis caused by mutagenic substance, and laid the foundation for future studies investigating their antigenotoxic potential in DNA oxidative damage therapy.https://www.mdpi.com/2076-3417/11/17/8142antioxidantscurcuminellagic acidgenotoxic stressoxidative stress |
| spellingShingle | Filomena Mottola Renata Finelli Concetta Iovine Maria Carannante Marianna Santonastaso Lucia Rocco Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood Cells Applied Sciences antioxidants curcumin ellagic acid genotoxic stress oxidative stress |
| title | Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood Cells |
| title_full | Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood Cells |
| title_fullStr | Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood Cells |
| title_full_unstemmed | Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood Cells |
| title_short | Anti-Genotoxicity Evaluation of Ellagic Acid and Curcumin—An <i>In Vitro</i> Study on Zebrafish Blood Cells |
| title_sort | anti genotoxicity evaluation of ellagic acid and curcumin an i in vitro i study on zebrafish blood cells |
| topic | antioxidants curcumin ellagic acid genotoxic stress oxidative stress |
| url | https://www.mdpi.com/2076-3417/11/17/8142 |
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