Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)
The European grapevine moth (EGVM) <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensi...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/11/1033 |
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| author | Ignacio Vicente-Díez Rubén Blanco-Pérez Maryam Chelkha Miguel Puelles Alicia Pou Raquel Campos-Herrera |
| author_facet | Ignacio Vicente-Díez Rubén Blanco-Pérez Maryam Chelkha Miguel Puelles Alicia Pou Raquel Campos-Herrera |
| author_sort | Ignacio Vicente-Díez |
| collection | DOAJ |
| description | The European grapevine moth (EGVM) <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensive pesticide use. The entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are well-known virulent agents against arthropod pests thanks to symbiotic bacteria in the genera <i>Xenorhabdus</i> and <i>Photorhabdus</i> (respectively) that produce natural products with insecticidal potential. Novel technological advances allow field applications of EPNs and those bioactive compounds as powerful bio-tools against aerial insect pests. This study aimed to determine the viability of four EPN species (<i>Steinernema feltiae</i>, <i>S. carpocapsae</i>, <i>S. riojaense</i>, and <i>Heterorhabditis bacteriophora</i>) as biological control agents against EGVM larval instars (L1, L3, and L5) and pupae. Additionally, the bioactive compounds from their four symbiotic bacteria (<i>Xenorhabdus bovienii</i>, <i>X. nematophila</i>, <i>X. kozodoii</i>, and <i>Photorhabdus laumondii</i> subsp. <i>laumondii,</i> respectively) were tested as unfiltered ferment (UF) and cell-free supernatant (CFS) against the EGVM larval instars L1 and L3. All of the EPN species showed the capability of killing EGVM during the larval and pupal stages, particularly <i>S. carpocapsae</i> (mortalities of ~50% for L1 and >75% for L3 and L5 in only two days), followed by efficacy by <i>S. feltiae</i>. Similarly, the bacterial bioactive compounds produced higher larval mortality at three days against L1 (>90%) than L3 (~50%), making the application of UF more virulent than the application of CFS. Our findings indicate that both steinernematid species and their symbiotic bacterial bioactive compounds could be considered for a novel agro-technological approach to control <i>L. botrana</i> in vineyards. Further research into co-formulation with adjuvants is required to expand their viability when implemented for aboveground grapevine application. |
| first_indexed | 2024-03-10T05:24:33Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T05:24:33Z |
| publishDate | 2021-11-01 |
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| series | Insects |
| spelling | doaj.art-435ab6c860b44dba8078356d0a1fa7d22023-11-22T23:47:28ZengMDPI AGInsects2075-44502021-11-011211103310.3390/insects12111033Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)Ignacio Vicente-Díez0Rubén Blanco-Pérez1Maryam Chelkha2Miguel Puelles3Alicia Pou4Raquel Campos-Herrera5Instituto de Ciencias de la Vid y del Vino (ICVV), Gobierno de La Rioja, CSIC, Universidad de La Rioja, Finca La Grajera Crta. Burgos Km. 6 Salida 13 Lo-20, 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV), Gobierno de La Rioja, CSIC, Universidad de La Rioja, Finca La Grajera Crta. Burgos Km. 6 Salida 13 Lo-20, 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV), Gobierno de La Rioja, CSIC, Universidad de La Rioja, Finca La Grajera Crta. Burgos Km. 6 Salida 13 Lo-20, 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV), Gobierno de La Rioja, CSIC, Universidad de La Rioja, Finca La Grajera Crta. Burgos Km. 6 Salida 13 Lo-20, 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV), Gobierno de La Rioja, CSIC, Universidad de La Rioja, Finca La Grajera Crta. Burgos Km. 6 Salida 13 Lo-20, 26007 Logroño, SpainInstituto de Ciencias de la Vid y del Vino (ICVV), Gobierno de La Rioja, CSIC, Universidad de La Rioja, Finca La Grajera Crta. Burgos Km. 6 Salida 13 Lo-20, 26007 Logroño, SpainThe European grapevine moth (EGVM) <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensive pesticide use. The entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are well-known virulent agents against arthropod pests thanks to symbiotic bacteria in the genera <i>Xenorhabdus</i> and <i>Photorhabdus</i> (respectively) that produce natural products with insecticidal potential. Novel technological advances allow field applications of EPNs and those bioactive compounds as powerful bio-tools against aerial insect pests. This study aimed to determine the viability of four EPN species (<i>Steinernema feltiae</i>, <i>S. carpocapsae</i>, <i>S. riojaense</i>, and <i>Heterorhabditis bacteriophora</i>) as biological control agents against EGVM larval instars (L1, L3, and L5) and pupae. Additionally, the bioactive compounds from their four symbiotic bacteria (<i>Xenorhabdus bovienii</i>, <i>X. nematophila</i>, <i>X. kozodoii</i>, and <i>Photorhabdus laumondii</i> subsp. <i>laumondii,</i> respectively) were tested as unfiltered ferment (UF) and cell-free supernatant (CFS) against the EGVM larval instars L1 and L3. All of the EPN species showed the capability of killing EGVM during the larval and pupal stages, particularly <i>S. carpocapsae</i> (mortalities of ~50% for L1 and >75% for L3 and L5 in only two days), followed by efficacy by <i>S. feltiae</i>. Similarly, the bacterial bioactive compounds produced higher larval mortality at three days against L1 (>90%) than L3 (~50%), making the application of UF more virulent than the application of CFS. Our findings indicate that both steinernematid species and their symbiotic bacterial bioactive compounds could be considered for a novel agro-technological approach to control <i>L. botrana</i> in vineyards. Further research into co-formulation with adjuvants is required to expand their viability when implemented for aboveground grapevine application.https://www.mdpi.com/2075-4450/12/11/1033cell-free supernatantgrape<i>Heterorhabditis</i><i>Photorhabdus</i><i>Steinernema</i>secondary metabolites |
| spellingShingle | Ignacio Vicente-Díez Rubén Blanco-Pérez Maryam Chelkha Miguel Puelles Alicia Pou Raquel Campos-Herrera Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) Insects cell-free supernatant grape <i>Heterorhabditis</i> <i>Photorhabdus</i> <i>Steinernema</i> secondary metabolites |
| title | Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) |
| title_full | Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) |
| title_fullStr | Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) |
| title_full_unstemmed | Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) |
| title_short | Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) |
| title_sort | exploring the use of entomopathogenic nematodes and the natural products derived from their symbiotic bacteria to control the grapevine moth i lobesia botrana i lepidoptera tortricidae |
| topic | cell-free supernatant grape <i>Heterorhabditis</i> <i>Photorhabdus</i> <i>Steinernema</i> secondary metabolites |
| url | https://www.mdpi.com/2075-4450/12/11/1033 |
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