Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality Traits
Peanut is an important oilseed and food crop worldwide; however, the development of new cultivars is limited by its remarkably low genetic variability. Therefore, in order to enhance peanut genetic variability, here, we treated two widely cultivated peanut genotypes, Huayu 22 and Yueyou 45, with dif...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/10/5/655 |
| _version_ | 1797568735514460160 |
|---|---|
| author | Tingting Chen Luping Huang Miaomiao Wang Yang Huang Ruier Zeng Xinyue Wang Leidi Wang Shubo Wan Lei Zhang |
| author_facet | Tingting Chen Luping Huang Miaomiao Wang Yang Huang Ruier Zeng Xinyue Wang Leidi Wang Shubo Wan Lei Zhang |
| author_sort | Tingting Chen |
| collection | DOAJ |
| description | Peanut is an important oilseed and food crop worldwide; however, the development of new cultivars is limited by its remarkably low genetic variability. Therefore, in order to enhance peanut genetic variability, here, we treated two widely cultivated peanut genotypes, Huayu 22 and Yueyou 45, with different concentrations of the mutagen ethyl methyl sulfonate (EMS) for different durations. Based on median lethal dose (LD50) value, optimal EMS treatment concentrations for each duration were identified for each genotype. Mutants induced by EMS differed in various phenotypic traits, including plant height, number of branches, leaf characteristics, and yield and quality in plants of the M2 generation. Moreover, we identified potentially useful mutants associated with dwarfism, leaf color and shape, high oil and/or protein content, seed size and testa color, among individuals of the M2 generation. Mutations were stably inherited in M3-generation individuals. In addition to their contribution to the study and elucidation of the mechanisms underlying the regulation of the expression of some important agronomic traits, the mutants obtained in this study provide valuable germplasm resources for use in peanut improvement programs. |
| first_indexed | 2024-03-10T20:01:10Z |
| format | Article |
| id | doaj.art-435caacb092c4d719e6a9adeec9739c7 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T20:01:10Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-435caacb092c4d719e6a9adeec9739c72023-11-19T23:32:40ZengMDPI AGAgronomy2073-43952020-05-0110565510.3390/agronomy10050655Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality TraitsTingting Chen0Luping Huang1Miaomiao Wang2Yang Huang3Ruier Zeng4Xinyue Wang5Leidi Wang6Shubo Wan7Lei Zhang8Guangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaBiotechnology Research Center, Shandong Academy of Agricultural Science, Jinan 250100, ChinaGuangdong Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaPeanut is an important oilseed and food crop worldwide; however, the development of new cultivars is limited by its remarkably low genetic variability. Therefore, in order to enhance peanut genetic variability, here, we treated two widely cultivated peanut genotypes, Huayu 22 and Yueyou 45, with different concentrations of the mutagen ethyl methyl sulfonate (EMS) for different durations. Based on median lethal dose (LD50) value, optimal EMS treatment concentrations for each duration were identified for each genotype. Mutants induced by EMS differed in various phenotypic traits, including plant height, number of branches, leaf characteristics, and yield and quality in plants of the M2 generation. Moreover, we identified potentially useful mutants associated with dwarfism, leaf color and shape, high oil and/or protein content, seed size and testa color, among individuals of the M2 generation. Mutations were stably inherited in M3-generation individuals. In addition to their contribution to the study and elucidation of the mechanisms underlying the regulation of the expression of some important agronomic traits, the mutants obtained in this study provide valuable germplasm resources for use in peanut improvement programs.https://www.mdpi.com/2073-4395/10/5/655<i>Arachis hypogaea</i> L.EMSmutantdwarfhigh proteinbreeding |
| spellingShingle | Tingting Chen Luping Huang Miaomiao Wang Yang Huang Ruier Zeng Xinyue Wang Leidi Wang Shubo Wan Lei Zhang Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality Traits Agronomy <i>Arachis hypogaea</i> L. EMS mutant dwarf high protein breeding |
| title | Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality Traits |
| title_full | Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality Traits |
| title_fullStr | Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality Traits |
| title_full_unstemmed | Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality Traits |
| title_short | Ethyl Methyl Sulfonate-Induced Mutagenesis and Its Effects on Peanut Agronomic, Yield and Quality Traits |
| title_sort | ethyl methyl sulfonate induced mutagenesis and its effects on peanut agronomic yield and quality traits |
| topic | <i>Arachis hypogaea</i> L. EMS mutant dwarf high protein breeding |
| url | https://www.mdpi.com/2073-4395/10/5/655 |
| work_keys_str_mv | AT tingtingchen ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT lupinghuang ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT miaomiaowang ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT yanghuang ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT ruierzeng ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT xinyuewang ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT leidiwang ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT shubowan ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits AT leizhang ethylmethylsulfonateinducedmutagenesisanditseffectsonpeanutagronomicyieldandqualitytraits |