Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in Saliva
Monitoring biomarkers is a great way to assess daily physical condition, and using saliva instead of blood samples is more advantageous as the process is simple and allows individuals to test themselves. In the present study, we analyzed the titers of neutralizing antibodies, IgG and secretory IgA (...
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MDPI AG
2023-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/167 |
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| author | Eiichi Tamiya Shuto Osaki Tomoko Tsuchihashi Hiromi Ushijima Keiichi Tsukinoki |
| author_facet | Eiichi Tamiya Shuto Osaki Tomoko Tsuchihashi Hiromi Ushijima Keiichi Tsukinoki |
| author_sort | Eiichi Tamiya |
| collection | DOAJ |
| description | Monitoring biomarkers is a great way to assess daily physical condition, and using saliva instead of blood samples is more advantageous as the process is simple and allows individuals to test themselves. In the present study, we analyzed the titers of neutralizing antibodies, IgG and secretory IgA (sIgA), in response to the SARS-CoV-2 vaccine, in saliva. A total of 19 saliva and serum samples were collected over a 10-month period 3 weeks after the first vaccine, 8 months after the second vaccine, and 1 month after the third vaccine. The ranges of antibody concentrations post-vaccination were: serum IgG: 81–15,000 U/mL, salivary IgG: 3.4–330 U/mL, and salivary IgA: 58–870 ng/mL. A sharp increase in salivary IgG levels was observed after the second vaccination. sIgA levels also showed an increasing trend. A correlation with trends in serum IgG levels was observed, indicating the possibility of using saliva to routinely assess vaccine efficacy. The electrochemical immunosensor assay developed in this study based on the gold-linked electrochemical immunoassay, and the antioxidant activity measurement based on luminol electrochemiluminescence (ECL), can be performed using portable devices, which would prove useful for individual-based diagnosis using saliva samples. |
| first_indexed | 2024-03-11T09:04:50Z |
| format | Article |
| id | doaj.art-435e4fd61c96448389bc20b895df02c3 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:04:50Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-435e4fd61c96448389bc20b895df02c32023-11-16T19:24:58ZengMDPI AGBiosensors2079-63742023-01-0113216710.3390/bios13020167Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in SalivaEiichi Tamiya0Shuto Osaki1Tomoko Tsuchihashi2Hiromi Ushijima3Keiichi Tsukinoki4Advanced Photonics and Biosensing Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology, Photonics Center, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Osaka, JapanAdvanced Photonics and Biosensing Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology, Photonics Center, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Osaka, JapanBioDevice Technology Ltd., 2-3 Asahidai, Nomi 923-1211, Ishikawa, JapanBioDevice Technology Ltd., 2-3 Asahidai, Nomi 923-1211, Ishikawa, JapanDepartment of Environmental Pathology, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka 238-0003, Kanagawa, JapanMonitoring biomarkers is a great way to assess daily physical condition, and using saliva instead of blood samples is more advantageous as the process is simple and allows individuals to test themselves. In the present study, we analyzed the titers of neutralizing antibodies, IgG and secretory IgA (sIgA), in response to the SARS-CoV-2 vaccine, in saliva. A total of 19 saliva and serum samples were collected over a 10-month period 3 weeks after the first vaccine, 8 months after the second vaccine, and 1 month after the third vaccine. The ranges of antibody concentrations post-vaccination were: serum IgG: 81–15,000 U/mL, salivary IgG: 3.4–330 U/mL, and salivary IgA: 58–870 ng/mL. A sharp increase in salivary IgG levels was observed after the second vaccination. sIgA levels also showed an increasing trend. A correlation with trends in serum IgG levels was observed, indicating the possibility of using saliva to routinely assess vaccine efficacy. The electrochemical immunosensor assay developed in this study based on the gold-linked electrochemical immunoassay, and the antioxidant activity measurement based on luminol electrochemiluminescence (ECL), can be performed using portable devices, which would prove useful for individual-based diagnosis using saliva samples.https://www.mdpi.com/2079-6374/13/2/167salivary biomarkersgold-linked electrochemical immunosensorelectrochemiluminescenceanti-SARS-CoV-2 neutralizing antibodiesantioxidant activitypoint-of-care diagnosis |
| spellingShingle | Eiichi Tamiya Shuto Osaki Tomoko Tsuchihashi Hiromi Ushijima Keiichi Tsukinoki Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in Saliva Biosensors salivary biomarkers gold-linked electrochemical immunosensor electrochemiluminescence anti-SARS-CoV-2 neutralizing antibodies antioxidant activity point-of-care diagnosis |
| title | Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in Saliva |
| title_full | Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in Saliva |
| title_fullStr | Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in Saliva |
| title_full_unstemmed | Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in Saliva |
| title_short | Point-of-Care Diagnostic Biosensors to Monitor Anti-SARS-CoV-2 Neutralizing IgG/sIgA Antibodies and Antioxidant Activity in Saliva |
| title_sort | point of care diagnostic biosensors to monitor anti sars cov 2 neutralizing igg siga antibodies and antioxidant activity in saliva |
| topic | salivary biomarkers gold-linked electrochemical immunosensor electrochemiluminescence anti-SARS-CoV-2 neutralizing antibodies antioxidant activity point-of-care diagnosis |
| url | https://www.mdpi.com/2079-6374/13/2/167 |
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