| Summary: | In this study, an efficient and reproducible plant
regeneration system for lotus (Nelumbo nucifera Gaertn.)
was established using shoot apical meristems from the
buds and one-week-old aseptically germinated embryos
as explants. Multiple shoot clumps were induced on
Murashige and Skoog (MS) basal medium supplemented
with various combinations of N6-Benzylaminopurine
(6-BA) and α-Naphthalene acetic acid (NAA). The
maximum response was obtained with 2.22 μM 6-BA, and
produced 21.33 shoots per explant after four weeks. After
five subcultures, multiple shoot clumps were transferred
to MS basal medium supplemented with various
combinations of 3-Indolebutyric acid (IBA), NAA and
sucrose for root induction. After four weeks, plantlets with
well-developed roots were achieved on MS basal medium
supplemented with 0.54 μM NAA and 30 g/L sucrose with
100% rooting rate. The successfully acclimated plantlets
were transferred to pots with the addition of 2 g/L KMnO4
into the soils, and finally fertile plants with much bigger
leaves were obtained in the greenhouse. The survival rate
was 97.33%.
|
|---|