In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb.
Diospyros melanoxylon Roxb. (D. melanoxylon) belongs to the family Ebenaceae and its leaves are very well known for making beedi throughout the World. The current study estimated the comparative extraction technique and its in-vitro antidiabetic prospective of the leaves of D. melanoxylon. Qualitati...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | Journal of Traditional and Complementary Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411017301311 |
| _version_ | 1818913277556555776 |
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| author | Md. Harun Al Rashid Sayani Majumder Vivekananda Mandal Subhash C. Mandal Rajarajan Amirthalingam Thandavarayan |
| author_facet | Md. Harun Al Rashid Sayani Majumder Vivekananda Mandal Subhash C. Mandal Rajarajan Amirthalingam Thandavarayan |
| author_sort | Md. Harun Al Rashid |
| collection | DOAJ |
| description | Diospyros melanoxylon Roxb. (D. melanoxylon) belongs to the family Ebenaceae and its leaves are very well known for making beedi throughout the World. The current study estimated the comparative extraction technique and its in-vitro antidiabetic prospective of the leaves of D. melanoxylon. Qualitative phytochemicals analysis of the samples from D. melanoxylon was carried out for the detection of secondary metabolites. Total phenolics, flavonoids, triterpenoids and tannins content of D. melanoxylon were estimated using colorimetric assay. Microwave-assisted extraction (MAE) technique with a low carbon output was observed for the speedy extraction of bioactive compounds obtained from Diospyros melanoxylon leaf extract. MAE produced a maximum yield of bioactive compounds which was found to be more efficient than ultrasound, soxhlet and maceration extraction. Qualitative HPLC analysis was performed for bioactive compounds. The in-vitro antidiabetic assay was performed using α-amylase and α-glucosidase inhibitory activity. In conclusion, the fractions exhibited the concentration-dependent inhibitory effect with significant (P < 0.0001) result. So the above performance might be accountable for the antidiabetic activity of D. Melanoxylon leaf extract due to presence of bioactive compounds. Keywords: D. melanoxylon, Extraction techniques, HPLC, Polyphenolics, Antidiabetic |
| first_indexed | 2024-12-19T23:27:56Z |
| format | Article |
| id | doaj.art-4363bec98c9d4f52a9cec8743c68a07c |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-19T23:27:56Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-4363bec98c9d4f52a9cec8743c68a07c2022-12-21T20:01:49ZengElsevierJournal of Traditional and Complementary Medicine2225-41102019-04-0192106118In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb.Md. Harun Al Rashid0Sayani Majumder1Vivekananda Mandal2Subhash C. Mandal3Rajarajan Amirthalingam Thandavarayan4Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India; Corresponding author.Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, IndiaInstitute of Pharmacy, Guru Ghasidas Central University, Bilaspur 495009, IndiaPharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, IndiaDepartment of Cardiovascular Sciences, Center for Cardiovascular Regeneration, Houston Methodist Research Institute, TX 77030, USADiospyros melanoxylon Roxb. (D. melanoxylon) belongs to the family Ebenaceae and its leaves are very well known for making beedi throughout the World. The current study estimated the comparative extraction technique and its in-vitro antidiabetic prospective of the leaves of D. melanoxylon. Qualitative phytochemicals analysis of the samples from D. melanoxylon was carried out for the detection of secondary metabolites. Total phenolics, flavonoids, triterpenoids and tannins content of D. melanoxylon were estimated using colorimetric assay. Microwave-assisted extraction (MAE) technique with a low carbon output was observed for the speedy extraction of bioactive compounds obtained from Diospyros melanoxylon leaf extract. MAE produced a maximum yield of bioactive compounds which was found to be more efficient than ultrasound, soxhlet and maceration extraction. Qualitative HPLC analysis was performed for bioactive compounds. The in-vitro antidiabetic assay was performed using α-amylase and α-glucosidase inhibitory activity. In conclusion, the fractions exhibited the concentration-dependent inhibitory effect with significant (P < 0.0001) result. So the above performance might be accountable for the antidiabetic activity of D. Melanoxylon leaf extract due to presence of bioactive compounds. Keywords: D. melanoxylon, Extraction techniques, HPLC, Polyphenolics, Antidiabetichttp://www.sciencedirect.com/science/article/pii/S2225411017301311 |
| spellingShingle | Md. Harun Al Rashid Sayani Majumder Vivekananda Mandal Subhash C. Mandal Rajarajan Amirthalingam Thandavarayan In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. Journal of Traditional and Complementary Medicine |
| title | In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. |
| title_full | In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. |
| title_fullStr | In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. |
| title_full_unstemmed | In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. |
| title_short | In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. |
| title_sort | in search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes the case diospyros melanoxylon roxb |
| url | http://www.sciencedirect.com/science/article/pii/S2225411017301311 |
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